AUTHOR=Wang Danni , Sun Hongzheng , Zhang Jiaqi , Huang Zhenyue , Li Congyang , Han Longsen , Xin Yongan , Tang Shoubin , Ge Juan , Wang Qiang TITLE=FKBP25 Regulates Meiotic Apparatus During Mouse Oocyte Maturation JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=Volume 9 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.625805 DOI=10.3389/fcell.2021.625805 ISSN=2296-634X ABSTRACT=FK506 binding proteins 25 (FKBP25) has been shown to function in ribosome biogenesis, chromatin organization, and microtubule stability in mitosis. However, its role in meiosis has not been addressed. Here, we report that oocytes with FKBP25 depletion display abnormal spindles assembly and chromosomes arrangement, with defective kinetochore-microtubule attachment. Consistent with this finding, aneuploidy incidence is also elevated in oocytes depleted of FKBP25. Importantly, FKBP25 protein level is significantly reduced in oocytes from old mice, and ectopic expression of FKBP25 could partly rescue the aging-associated meiotic defects. In addition, by employing site-specific mutagenesis, we identify that serine 163 is a major, if not unique, phosphorylation site modulating the action of FKBP25 on meiotic maturation. In summary, our data indicate that FKBP25 is a key factor for determining oocyte quality, and may mediate the effects of maternal aging on female reproduction.