AUTHOR=Zhang Xingyu , Gao Yunqian , Zhang Xiaoping , Zhang Xiaoqing , Xiang Ying , Fu Qihua , Wang Bo , Xu Zhuoming 

TITLE=FGD5-AS1 Is a Hub lncRNA ceRNA in Hearts With Tetralogy of Fallot Which Regulates Congenital Heart Disease Genes Transcriptionally and Epigenetically

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.630634

DOI=10.3389/fcell.2021.630634

ISSN=2296-634X

ABSTRACT=Heart development requires robust gene regulation, and the related disruption could lead to congenital heart disease (CHD). To gain insights into regulation of gene expression in CHD, we obtained expression profiles of lncRNAs and mRNAs in 22 heart tissue samples with tetralogy of Fallot (TOF) through strand-specific transcriptomic analysis. Using a causal inference framework based on the expression correlations and validated miRNA-lncRNA/mRNA evidences, we constructed the ceRNA (competing endogenous RNA) mediated network driven by lncRNAs. Four lncRNAs (FGD5-AS1, lnc-GNB4-1, lnc-PDK3-1 and lnc-SAMD5-1) were identified as hub lncRNAs in the network. FGD5-AS1 was selected for further study since all its targets were CHD related genes (NRAS, PTEN and SMAD4). Both FGD5-AS1 and SMAD4 could bind with hsa-miR-421, which has been validated using dual-luciferase reporter assay. Knockdown of FGD5-AS1 not only significantly reduced PTEN and SMAD4 expression in HEK 293 and the fetal heart cell line (CCC-HEH-2), but also increased the transcription of its interacted miRNAs in a cell specific way. Besides ceRNA mechanism, RNAseq and ATACseq results showed that FGD5-AS1 might play repression roles in heart development by transcriptionally regulating CHD related genes.
In conclusion, we identified a ceRNA network driven by lncRNAs in heart tissues of TOF patients. Furthermore, we proved that FGD5-AS1, one candidate hub lncRNA in the TOF heart ceRNA network, regulates multiple genes transcriptionally and epigenetically.