AUTHOR=Zhang Eric , Liu Yanwen , Han Chaoshan , Fan Chengming , Wang Lu , Chen Wangping , Du Yipeng , Han Dunzheng , Arnone Baron , Xu Shiyue , Wei Yuhua , Mobley James , Qin Gangjian TITLE=Visualization and Identification of Bioorthogonally Labeled Exosome Proteins Following Systemic Administration in Mice JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=Volume 9 - 2021 YEAR=2021 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.657456 DOI=10.3389/fcell.2021.657456 ISSN=2296-634X ABSTRACT=
Exosomes transport biologically active cargo (e.g., proteins and microRNA) between cells, including many of the paracrine factors that mediate the beneficial effects associated with stem-cell therapy. Stem cell derived exosomes, in particular mesenchymal stem cells (MSCs), have been shown previously to largely replicate the therapeutic activity associated with the cells themselves, which suggests that exosomes may be a useful cell-free alternative for the treatment of cardiovascular disorders. However, the mechanisms that govern how exosomes home to damaged cells and tissues or the uptake and distribution of exosomal cargo are poorly characterized, because techniques for distinguishing between exosomal proteins and proteins in the targeted tissues are lacking. Here, we report the development of an