AUTHOR=Yang Jiani , Huang Shan , Cheng Shanshan , Jin Yue , Zhang Nan , Wang Yu 

TITLE=Application of Ovarian Cancer Organoids in Precision Medicine: Key Challenges and Current Opportunities

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.701429

DOI=10.3389/fcell.2021.701429

ISSN=2296-634X

ABSTRACT=Ovarian cancer (OC) is the leading cause of death among gynecologic malignances. Over past decades, human-derived models have advanced from monolayer cell cultures to three-dimensional (3D) organoids that could faithfully recapitulate biological characteristics and tumor heterogeneity of primary tissues. As a complement of previous studies based on cell lines or xenografts, organoids provide a 3D platform for mutation-carcinogenesis modeling, high-throughput drug screening, genetic engineering, and bio-banking, which might fulfill the gap between basic research and clinical practice. Stepwise, cutting-edge bioengineering techniques of organoid-on-a-chip and 3D-bioprinting might converge current challenges and contribute to personalized therapy. We comprehensively reviewed advantages, challenges, and translational potential of OC organoids. Undeniably, organoids represent an excellent near-physiological platform for OC, paving the way for precision medicine implementation. Future efforts will doubtlessly bring this innovative technique from bench to bedside.