AUTHOR=Xie Jinghe , Guo Tingting , Zhong Zhiyong , Wang Ning , Liang Yan , Zeng Weiping , Liu Shoupei , Chen Qicong , Tang Xianglian , Wu Haibin , Zhang Shuai , Ma Keqiang , Wang Bailin , Ou Yimeng , Gu Weili , Chen Honglin , Qiu Yaqi , Duan Yuyou 

TITLE=ITGB1 Drives Hepatocellular Carcinoma Progression by Modulating Cell Cycle Process Through PXN/YWHAZ/AKT Pathways

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.711149

DOI=10.3389/fcell.2021.711149

ISSN=2296-634X

ABSTRACT=Integrin β1 (ITGB1), which acts as an extracellular matrix (ECM) receptor, has gained increasing attention as a therapeutic target for the treatment of hepatocellular carcinoma (HCC). However, the underpinning mechanism of how ITGB1 drives HCC progression remains elusive. In this study, we first found that ITGB1 expression was significantly higher in HCC tissues than in normal controls by bioinformatic analysis. Furthermore, bioinformatic analysis revealed that Paxillin (PXN) and 14-3-3 protein zeta (YWHAZ) are the molecules participated in ITGB1-regulated HCC tumor cell cycle progression. Indeed, immunohistochemistry (IHC) revealed that ITGB1, Paxillin and YWHAZ were strongly upregulated in paired HCC tissue compared with adjacent normal tissues. Notably, the inhibition of ITGB1 expression by small interfering RNA (siRNA) resulted in the downregulated expression of PXN and YWHAZ in primary HCC cells, as assessed by qRT-PCR, Western blot and immunostaining. In addition, ITGB1 knockdown markedly impaired the aggressive behavior of HCC tumor cells and retarded cell cycle progression as determined by cell migration assay, drug-resistance analysis, colony formation assay, qRT-PCR and cell cycle analysis as well as cell viability measurements. More importantly, we proved that xenograft ITGB1high tumors grew more rapidly than ITGB1low tumors. Altogether, our study showed that ITGB1/PXN/YWHAZ/AKT axis enhances HCC progression by accelerating cell cycle process, which offers a promising approach to halt HCC tumor growth.