AUTHOR=Gu Huijie , Huang Zhongyue , Zhou Kaifeng , Chen Guangnan , Bian Chong , Xu Jun , Yin Xiaofan 

TITLE=Expression Profile Analysis of Long Non-coding RNA in OVX Models-Derived BMSCs for Postmenopausal Osteoporosis by RNA Sequencing and Bioinformatics

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.719851

DOI=10.3389/fcell.2021.719851

ISSN=2296-634X

ABSTRACT=Osteoporosis (OP) has the characteristics of a systematically impaired bone mass, strength and microstructure. Long non-coding RNAs (lncRNAs) are longer than 200 nt, and their functions in osteoporosis is yet not completely understood. We firstly harvested the bone marrow mesenchymal stem cells (BMSCs) from ovariectomy (OVX) and sham mice. Then we systematically analyzed the differential expressions of lncRNAs and mRNAs, and constructed lncRNA-mRNA co-expression network, in order to identify the function of lncRNA in osteoporosis. Totally, we screened 743 lncRNAs (461 upregulated lncRNAs and 282 downregulated lncRNAs) and 240 mRNAs (128 upregulated and 112 downregulated) with significantly differential expressions in OP compared to normal. We conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional analyses to investigate the functions and pathways of the differential expression of mRNAs, a co-expressed network of lncRNA/mRNA. qPCR validated that the expressions of ONMMUT096150.1, NONMMUT083450.1, NONMMUT029743.2 were all downregulated, whereas NONMMUT026970.2, NONMMUT051734.2, NONMMUT003617.2 and NONMMUT034049.2 were all upregulated in the OVX group. NONMMUT096150.1, as a key lncRNA in OP, was identified to modulate the adipogenesis of BMSCs. Further analysis suggested that NONMMUT096150.1 might modulate the adipogenesis of BMSCs via the PPAR signaling pathway, AMPK signaling pathway, the lipolysis regulation in adipocyte and adipocytokine signaling pathway. Our study expands the understanding of lncRNA in the pathogenesis of OP.