AUTHOR=Chen Jieqing , Liao Xinhui , Cheng Jianli , Su Ganglin , Yuan Fen , Zhang Zhongfu , Wu Jianting , Mei Hongbing , Tan Wanlong 

TITLE=Targeted Methylation of the LncRNA NEAT1 Suppresses Malignancy of Renal Cell Carcinoma

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.777349

DOI=10.3389/fcell.2021.777349

ISSN=2296-634X

ABSTRACT=Long-chain non-coding RNA (LncRNA) has been found to play an important role in the 
regulation of the occurrence and progression of renal cell carcinoma (RCC). In this study, 
we demonstrated that LncRNA NEAT1 expression and m6A methylation level was 
decreased in RCC tissues. Furthermore, we found that the downregulated expression level 
of LncRNA NEAT1 was a prognostic indicator for patients with RCC. Then we used 
CRIPSR/dCas13b-METTL3 to methylate LncRNA NEAT1 in 786-O and OSRC RCC cells. 
We found the expression levels of LncRNA NEAT1 were upregulated after methylated by 
dCas13b-METTL3 in 786-O and OSRC RCC cell. And the proliferation and migration ability 
of RCC cells was decreased after methylated LncRNA NEAT1. Finally, we examined the 
effect of LncRNA NEAT1 hypermethylation on the transcriptome. We found differentially 
expressed genes in RCC cells were associated with “cGMP-PKG signaling pathway”, “Cell 
adhesion molecules” and “Pathways in cancer”. In conclusion, CRISPR/Cas13b-METTL3 
targeting LncRNA NEAT1 m6A methylation activates LncRNA NEAT1 expression and
might provide a new light on the treatment for RCC