AUTHOR=Ding Hongmei , Jiang Fei , Deng Lifeng , Wang Juan , Wang Ping , Ji Mintao , Li Jie , Shi Weiqiang , Pei Yufang , Li Jiafu , Zhang Yue , Zhang Zengli , Chen Youguo , Li Bingyan 

TITLE=Prediction of Clinical Outcome in Endometrial Carcinoma Based on a 3-lncRNA Signature

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 9 - 2021

YEAR=2022

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.814456

DOI=10.3389/fcell.2021.814456

ISSN=2296-634X

ABSTRACT=In present study, we constructed a new lncRNA model to predict the prognosis of patients with Endometrial Carcinoma (EC). Univariate COX regression and LASSO model were selected to find the 8-independent prognostic lncRNAs for EC patient from TCGA. Furthermore, the risk score of the 3-lncRNAs signature for overall survival was identified as: CTD-2377D24.6 expression × 0.206+ RP4-616B8.5 × 0.341 + RP11-389G6.3 × 0.343 by multivariate Cox regression analysis. According to the median cut off value of this prognostic signature, patients were divided into low-risk and high-risk sets, and demonstrated that the low-risk set had a higher survival rate than that of the high-risk set. In addition, the 3-lncRNAs signature was closely linked with histological subtype and advanced clinical stage in EC patients. Moreover, our clinical samples also confirmed that RP4-616B8.5, RP11-389G6.3 and CTD-2377D24.6 levels were increased in tumor tissues by qRT-PCR and in situ hybridization. Intriguingly, the p-value of combined 3-lncRNAs was lower than that of each lncRNA, indicating that the 3-lncRNAs signature also showed higher performance in EC tissue than paracancerous. Functional analysis revealed that cortactin might be involved in the mechanism of 3-lncRNA signatures. These findings provide the first hint that a panel of lncRNAs, may play a critical role in the initiation and metastasis of EC, indicating a new signature for early diagnosis and therapeutic strategy of EC.