AUTHOR=Jiang Piao , Liang Bin , Zhang Zhen , Fan Bing , Zeng Lin , Zhou Zhiyong , Mao Zhifang , Lin Qing , Yao Weirong , Shen Qinglin 

TITLE=MicroRNA-146a-5p induces cell cycle arrest and enhances apoptosis in gastric cancer via targeting CDC14A

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1181628

DOI=10.3389/fcell.2023.1181628

ISSN=2296-634X

ABSTRACT=Objective：The study was to investigate the expression of miRNA-146a-5p in gastric cancer (GC) tissues and the paired nonmalignant tissues, to explore the influences of miRNA-146a-5p in the cell biological behavior of MKN-28 cells (high metastatic human gastric cancer cells), and to identify the function of abnormal expression of its target gene Cell Division Cycle 14 Homolog A (CDC14A) in GC. 
Methods: We analyzed the expression of miRNA-146a-5p in GC tissues with formalin fixed and paraffin embedded (FFPE) using microarray and quantitative real-time Polymerase Chain Reaction (qRT-PCR). Then we employed Cell Counting Kit-8 (CCK-8) assays, cell cycle assays, as well as apoptosis analysis to uncover latent function of miRNA-146a-5p toward MKN-28 human GC cells. The luciferase reporter system validated straight-forward targets. 
Results: MiRNA-146a-5p levels were examined among the majority of primary GC and several GC cells lines. Abnormal levels of miRNA-146a-5p stimulated proliferation and inhibited apoptosis in vivo. miRNA-146a-5p was demonstrated a straight act on its 3'-untranslated region (3'-UTR) of CDC14A mRNA, accordingly decreasing the corresponding mRNA along with protein contents. An inverse correlation between CDC14A and miRNA-146a-5p was observed. 
Conclusions: These data suggest miRNA-146a-5p may contribute to inducing cell cycle arrest together with prompting GC cell apoptosis via straightly targeting CDC14A. miRNA-146a-5p may be implicated in the occurrence and development of GC.