AUTHOR=Yi Mingliang , Wang Mingyue , Xu Yongjie , Cao Zhikun , Ling Yinghui , Zhang Zijun , Cao Hongguo TITLE=CRISPR-based m6A modification and its potential applications in telomerase regulation JOURNAL=Frontiers in Cell and Developmental Biology VOLUME=Volume 11 - 2023 YEAR=2023 URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1200734 DOI=10.3389/fcell.2023.1200734 ISSN=2296-634X ABSTRACT=Telomerase determines cell lifespan by controlling chromosome stability and cell viability, m 6 A epigenetic modification plays an important role in the regulation of telomerase activity. Using CRISPR epigenome editing to analyze specific m 6 A modification sites in telomerase will provide an important tool for analyzing the molecular mechanism of m 6 A modification regulating telomerase activity. In this review, we clarified the relevant applications of CRISPR system, paid special attention to the regulation of m 6 A modification in stem cells and cancer cells based on CRISPR system, emphasized the regulation of m 6 A modification on telomerase activity, pointed out that m 6 A modification sites regulate telomerase activity, and discussed strategies based on telomerase activity and disease treatment, which are helpful to promote the research of anti-aging and tumor related diseases.