AUTHOR=Soshnikova Nataliya V. , Azieva Asya M. , Klimenko Nataliya S. , Khamidullina Alvina I. , Feoktistov Alexey V. , Sheynov Andrey A. , Brechalov Alexander V. , Tatarskiy Victor V. , Georgieva Sofia G. 

TITLE=A novel chromatin-remodeling complex variant, dcPBAF, is involved in maintaining transcription in differentiated neurons

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.1271598

DOI=10.3389/fcell.2023.1271598

ISSN=2296-634X

ABSTRACT=The PBAF chromatin-remodeling complex is essential for transcription in mammalian cells.Here we describe a novel variant of the PBAF complex from differentiated neuronal cells, called dcPBAF that differs from the canonical PBAF existing in proliferating neuroblasts. We describe that in differentiated adult neurons a specific subunit of PBAF, PHF10, is replaced by a PHF10 isoform that is lacking N-terminal and C-terminal domains (called PHF10D). In addition, dcPBAF does not contain the canonical BRD7 subunit. DcPBAF binds promoters of the actively transcribed neuron-specific and housekeeping genes in terminally differentiated neurons of adult mice. Furthermore, in differentiated human neuronal cells the PHF10D-containing dcPBAF maintains a high transcriptional level at several neuron-specific genes.