AUTHOR=Didaskalou Stylianos , Efstathiou Christos , Galtsidis Sotirios , Kesisova Ilοna , Halavatyi Aliaksandr , Elmali Tountzai , Tsolou Avgi , Girod Andreas , Koffa Maria 

TITLE=HURP localization in metaphase is the result of a multi-step process requiring its phosphorylation at Ser627 residue

JOURNAL=Frontiers in Cell and Developmental Biology

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2023.981425

DOI=10.3389/fcell.2023.981425

ISSN=2296-634X

ABSTRACT=Interactions among spindle assembly factors play a crucial role for a faultless mitotic spindle formation.Here, by exploring the spatiotemporal dynamics of HURP, a Ran-GTP regulated microtubule associated protein, we show that HURP bound on spindle microtubules can move in both directions: poleward and equatorward. We suggest that the proper chromosome-proximal localization of HURP on kinetochore fibers is the result of a multi-step process that involves binding to different interacting partners. Moreover, our results indicate that this process is regulated by the phosphorylation of HURP at Ser627 residue.