AUTHOR=Singh Rajnish Prakash , Halaka Diana Abu , Hayouka Zvi , Tirosh Oren 

TITLE=High-Fat Diet Induced Alteration of Mice Microbiota and the Functional Ability to Utilize Fructooligosaccharide for Ethanol Production

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 10 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2020.00376

DOI=10.3389/fcimb.2020.00376

ISSN=2235-2988

ABSTRACT=High fat diet (HFD) leads to enhancement in various parameters of mice like weight, fasting glucose levels, adipose tissue and also the liver weight in male C57 BL/6 J mice. Additionally, high fat diet causes the severe liver damages with significant increase in the level of aspartate amino transferase (AST) and alanine transaminase (ALT). The variations of microbiota induced by different diet were analyzed by Illumina MiSeq plateform with sequencing of 16S rRNA gene and QIIME pipeline was used. Microbiota analysis suggests that phylum Proteobacteria and Firmicutes were found to be higher in high fat diet groups as compared to mice fed with normal diet (ND). At the genus level, Bacteriodes showed higher population in HFD diet. Bacterial strains belonging to Enterobacteriaceae like Escherichia, Klebsiella and Shigella sp. were also dominant in HFD treatments. Furthermore, we explored the effects of ethanol production in-vitro with supplementation of dietary fibers following inoculation of ND and HFD microbiotas. HFD microbiota of caecum and feces showed high level (P < 0.05) of ethanol production with 2% fructooligosaccharide (FOS) as compared to 2% galactomannan. Microbial fermentation also generated the short-chain fatty acids (SCFAs), such as acetate, propionate, and butyrate. High levels (P < 0.05) of propionate were found after fermentation of FOS with HFD caecum and fecal microbiota. The present study highlights the HFD induced population of phylum Proteobacteria and genus Bacteriodes for ethanol production using FOS as a dietary supplement and these findings may imply on the harmful effect of HFD even at the microbiota level.