AUTHOR=Zhang Tan , Zhang Sina , Jin Chen , Lin Zixia , Deng Tuo , Xie Xiaozai , Deng Liming , Li Xueyan , Ma Jun , Ding Xiwei , Liu Yaming , Shan Yunfeng , Yu Zhengping , Wang Yi , Chen Gang , Li Jialiang 

TITLE=A Predictive Model Based on the Gut Microbiota Improves the Diagnostic Effect in Patients With Cholangiocarcinoma

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 11 - 2021

YEAR=2021

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2021.751795

DOI=10.3389/fcimb.2021.751795

ISSN=2235-2988

ABSTRACT=Cholangiocarcinoma (CCA) is a hepatic malignant tumor with poor prognosis, which needs early diagnosis urgently. The gut microbiota has been shown a crucial role in the progression of liver cancer. Here, we explored a B-F-R genera model of gut microbiota for CCA early diagnosis. A case control study collecting the feces samples of 40 healthy controls, 53 CCA patients and 47 cholelithiasis (cancer-free, CF) patients was carried out in the same period. The involved gut microbiota and the its diversity were accessed with 16S rDNA sequencing, and the gut microbiota characteristics were evaluated according to alpha diversity and beta diversity. 4 enriched genera in CCA cohort, 8 enriched genera in the CF cohort constitute an overall different microbial community composition (p=0.001), in which, CCA and control participants have higher Chao1, observed number and Shannon diversity than those of CF individuals (p<0.01 for each index). The B-F-R genera model with better diagnostic value than CA19-9, covers genra Burkholderia-Caballeronia-Paraburkholderia, Faecalibacteriumwere and Ruminococcus_1, was identified by Random Forest and STAMP to distinguish CCA patients from healthy control (AUC=0.973, 95%CI=0.932-1.0). Further, Correlative analysis found genus Burkholderia-Caballeronia-Paraburkholderia and BMI were positively correlated. The significantly different microbiomes between cholelithiasis and CCA were found via PCoA and LEfSe, and venn diagram and LEfSe were utilized to identify 4 genera by comparing microbial compositions among patients with malignant obstructive jaundice (MOJ-Y) or not (MOJ-N). In brief, our findings suggest that gut microbiota vary from benign and malignant hepatobiliary diseases to normal people, and provide evidence supporting gut microbiota to be a non-invasive biomarker for early diagnosis of CCA.