AUTHOR=Zhao Hongyu , Chen Rufeng , Zheng Dongxue , Xiong Feng , Jia Fan , Liu Jinyuan , Zhang Lili , Zhang Nana , Zhu Shiqin , Liu Yongmei , Zhao Linhua , Liu Xinmin 

TITLE=Modified Banxia Xiexin Decoction Ameliorates Polycystic Ovarian Syndrome With Insulin Resistance by Regulating Intestinal Microbiota

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 12 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.854796

DOI=10.3389/fcimb.2022.854796

ISSN=2235-2988

ABSTRACT=ABSTRACT
Objective: To analyze the characteristics of intestinal flora of polycystic ovarian syndrome with insulin resistance (PCOS-IR), and explore the possible mechanism of supplemented Banxia Xiexin Decoction in the treatment of PCOS-IR. 
Methods: A total of 17 SPF female SD rats, aged 21 days were selected and randomly divided into control group (group Z, n=6), model group (group M, n=6) and treatment group (group A, n=5). PCOS-IR model rats were induced by letrozole combined with high-fat diet. PCOS-IR rats were treated with supplemented Banxia Xiexin Decoction for 2 weeks, then the reproductive, metabolic, inflammatory indices and intestinal microflora of rats in different groups were compared.
Results:The PCOS-IR model rats induced by letrozole combined with high-fat diet had the imbalance of intestinal flora, and the enriched flora were mainly Class Coriobacteria, Order Clostridiales and Genus Clostridium_ sensu_ stricto_ 1. Supplemented Banxia Xiexin Decoction can improve intestinal flora imbalance, and can significantly increase the abundance of Phylum Verrucomicrobiota, Phylum Proteobacteria, Genus Akkermansia, and Genus Blautia. 
Conclusion: Clostridium_sensu_stricto_1 may be the key pathogenic bacteria of PCOS-IR; Supplemented Banxia Xiexin Decoction may treat PCOS-IR by improving intestinal flora imbalance and regulating metabolic disorders.