AUTHOR=Chevignon Germain , Dotto-Maurel Aurélie , Serpin Delphine , Chollet Bruno , Arzul Isabelle 

TITLE=De Novo Transcriptome Assembly and Analysis of the Flat Oyster Pathogenic Protozoa Bonamia Ostreae

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 12 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.921136

DOI=10.3389/fcimb.2022.921136

ISSN=2235-2988

ABSTRACT=The flat oyster Ostrea edulis is an oyster species native to Europe.  It has declined to functional extinction in many areas of the NE Atlantic since several decades. Factors explaining this decline include over exploitation of natural populations and diseases like bonamiosis, regulated at the EU and world scales and caused by the intracellular protozoan parasite Bonamia ostreae. To date very few sequences information is available for this Haplosporidian species. We present here the first transcriptome of B. ostreae. Because this protozoa is not yet cultivable, it remains extremely challenging to obtain high quality -omic data. Thanks to a specific parasite isolation protocol and a dedicated bioinformatic pipeline we were able to obtain a high-quality transcriptome for an intracellular marine micro-eukaryote, which will be very helpful to better understand its biology and to consider the development of new relevant diagnostic tools.