AUTHOR=Kang Ni , Zhang Yong , Xue Fei , Duan Jinyu , Chen Fan , Cai Yu , Luan Qingxian 

TITLE=
Periodontitis induced by Porphyromonas gingivalis drives impaired glucose metabolism in mice


JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 12 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2022.998600

DOI=10.3389/fcimb.2022.998600

ISSN=2235-2988

ABSTRACT=Periodontitis has been demonstrated to bidirectionally associated with diabetes and has been recognized as a complication of diabetes. As a periodontal pathogen, Porphyromonas gingivalis (Pg) is a possible pathogen linking periodontal disease and systemic diseases. It has also been found to be participated in the occurrence and development of diabetes. In this study, six-week-old male C57BL/6 mice were orally administered P. gingivalis strain ATCC381 for 22 weeks. Histological analysis of the gingival tissue and quantified alveolar bone loss analysis were performed to evaluate periodontal destruction. Body weight, fasting glucose, glucose tolerance test (GTT) and insulin tolerance test (ITT) were used to evaluate glucose metabolism disorder. We then analyzed the expression profile of inflammatory cytokines and chemokines in gingival tissue, the liver, and adipose tissue, as well as in serum. The results showed that mice in the Pg administration group developed apparent gingival inflammation and more alveolar bone loss compared to the control group. After 22 weeks of Pg infection, significant differences were observed at 30 and 60 min for the GTT and at 15 min for the ITT. Pg administered mice showed an increase in mRNA expression levels of pro-inflammatory cytokines (TNF-α, IL-6, IL-17 and IL-23) and chemokines (CCL2, CCL8 and CXCL10) in gingiva and serum. Glucose metabolism related genes’ expression levels were also changed in the liver and adipose tissue. Our results indicate that oral administration of Pg can induce changes in inflammatory cytokines and chemokines in gingiva and blood, can lead to alveolar bone loss, can lead to inflammatory changes in liver and adipose tissues, and can promote glucose metabolism disorder in mice.