AUTHOR=de Melo Camylla Carvalho , de Sousa Bruna Rodrigues , da Costa Gisela Lara , Oliveira Manoel Marques Evangelista , de Lima-Neto Reginaldo Gonçalves
TITLE=Colonized patients by Candida auris: Third and largest outbreak in Brazil and impact of biofilm formation
JOURNAL=Frontiers in Cellular and Infection Microbiology
VOLUME=Volume 13 - 2023
YEAR=2023
URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1033707
DOI=10.3389/fcimb.2023.1033707
ISSN=2235-2988
ABSTRACT=ObjectiveTo describe the clinical–epidemiological features of patients colonized by Candida auris in the largest outbreak in Brazil and to show the biofilm formation capacity of yeast strains.
MethodsClinical yeasts suspected of C. auris isolated from urine and surveillance samples were seeded on chromogenic media at 30°C and Sabouraud agar at 42°C. matrix-assisted laser desorption/ionization–time of flight mass spectometry was used for reliable identification. After proteomic confirmation, the genomic approach and culture on Chromagar Candida Plus media were carried out. Biofilm formation was investigated based on metabolic activity, and the clinical–epidemiological profile of patients was described.
ResultsA total of 11 C. auris clinical yeasts from nine patients were identified between the end of December 2021 and March 2022. Two clinical yeasts were isolates from urine and nine clinical yeasts were isolates from axillary and inguinal surveillance swabs. No case is related to previous Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, all the yeasts showed a high ability of biofilm formation.
ConclusionC. auris requires great vigilance as its high capacity to colonize and form biofilms contributes to its dissemination. The rapid and precise identification of this species is essential for the management, control, and prevention of infections.