AUTHOR=Cai Yuanqing , Ding Haiqi , Chen Xiaoqing , Chen Yang , Huang Changyu , Zhang Chaofan , Huang Zida , Huang Ying , Li Wenbo , Zhang Wenming , Fang Xinyu 

TITLE=Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 13 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1089919

DOI=10.3389/fcimb.2023.1089919

ISSN=2235-2988

ABSTRACT=The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized and optimized procedure for Mycoplasma PJI presently. In the present study, we have reported the largest number of Mycoplasma PJI that were precisely diagnosed by metagenomic next-generation sequencing (mNGS) and verified by optimized microbial culture methods and 16S PCR polymerase chain reaction (PCR). The positive rate of optimized microbial culture methods and 16S PCR in the detection of Mycoplasma PJI was 57.14% and 71.43%, respectively. The infections were well controlled by targeted treatment in all cases. Finally, we concluded a standardized and optimized procedure based on mNGS for the diagnosis of Mycoplasma PJI, which might be provided as a novel diagnostic strategy for rare bacterial PJI.