AUTHOR=Xu Ye , Xu Jiabao , Liu Tong , Liu Peiwen , Chen Xiao-Guang 

TITLE=Metagenomic analysis reveals the virome profiles of Aedes albopictus in Guangzhou, China

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 13 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1133120

DOI=10.3389/fcimb.2023.1133120

ISSN=2235-2988

ABSTRACT=<sec><title>Introduction</title><p><italic>Aedes albopictus</italic> is an aggressive invasive mosquito species widely distributed around the world, and it is also a known vector of arboviruses. Virus metagenomics and RNA interference (RNAi) are important in studying the biology and antiviral defense of <italic>Ae. albopictus</italic>. However, the virome and potential transmission of plant viruses by <italic>Ae. albopictus</italic> remain understudied.</p></sec><sec><title>Methods</title><p>Mosquito samples of <italic>Ae. albopictus</italic> were collected from Guangzhou, China, and small RNA sequencing was performed. Raw data were filtered, and virus-associated contigs were generated using VirusDetect. The small RNA profiles were analyzed, and maximum-likelihood phylogenetic trees were constructed.</p></sec><sec><title>Results</title><p>The small RNA sequencing of pooled <italic>Ae. albopictus</italic> revealed the presence of five known viruses, including Wenzhou sobemo-like virus 4, mosquito nodavirus, Aedes flavivirus, Hubei chryso-like virus 1, and Tobacco rattle virus RNA1. Additionally, 21 new viruses that had not been previously reported were identified. The mapping of reads and contig assembly provided insights into the viral diversity and genomic characteristics of these viruses. Field survey confirmed the detection of the identified viruses in <italic>Ae. albopictus</italic> collected from Guangzhou.</p></sec><sec><title>Discussion</title><p>The comprehensive analysis of the virus metagenomics of <italic>Ae. albopictus</italic> in this study sheds light on the diversity and prevalence of viruses in mosquito populations. The presence of known and novel viruses highlights the need for continued surveillance and investigation into their potential impact on public health. The findings also emphasize the importance of understanding the virome and potential transmission of plant viruses by <italic>Ae. albopictus</italic>.</p></sec><sec><title>Conclusion</title><p>This study provides valuable insights into the virome of <italic>Ae. albopictus</italic> and its potential role as a vector for both known and novel viruses. Further research is needed to expand the sample size, explore additional viruses, and investigate the implications for public health.</p></sec>