AUTHOR=Song Wei-Jie , Gao Jun , Huang Ji-Wei , Liu Yuan , Long Zhi , He Le-Ye 

TITLE=Is type III prostatitis also associated with bacterial infection?

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 13 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1189081

DOI=10.3389/fcimb.2023.1189081

ISSN=2235-2988

ABSTRACT=To explore whether type Ⅲ prostatitis is related to bacterial infection by detecting the composition and function of microorganisms in expressed prostatic secretion (EPS) of patients with chronic prostatitis (CP) and healthy people. Methods: According to the inclusion and exclusion criteria, 57 subjects were included in our study, divided into the healthy group, type Ⅱ prostatitis group, and type Ⅲ prostatitis group. 16s rRNA sequencing technique was used to detect and analyze the microbial composition of EPS in each group. Additionally, the metagenomics sequencing technique was used to further explore the function of different bacteria in the type Ⅲ prostatitis group. Data analysis was performed by bioinformatics software, and the results were statistically significant when P<0.05. Results: Many microorganisms exist in EPS in both CP patients and healthy populations. However, the relative abundance of Pseudomonas, Haemophilus, Sneathia, Allobaculum, and Enterococcus in CP patients (including type Ⅱ and Ⅲ) were significantly different. Still, the relative abundance of different bacteria in type Ⅱ prostatitis patients was much higher than in type Ⅲ. The metagenomics sequencing results for the type Ⅲ prostatitis group showed that the different bacteria had certain biological functions. Conclusions: Based on our sequencing results and previous studies, we suggest that type Ⅲprostatitis may also be caused by bacterial infection.