Two new species of Scytinostroma (Russulales, Basidiomycota) in Southwest China

Abstract

Two new species of Scytinostroma viz. S. acystidiatum and S. macrospermum, are described from southwest China. Phylogeny based on ITS + nLSU dataset demonstrates that samples of the two species form two independent lineages and are different in morphology from the existing species of Scytinostroma. Scytinostroma acystidiatum is characterized by resupinate, coriaceous basidiomata with cream to pale yellow hymenophore, a dimitic hyphal structure with generative hyphae bearing simple septa, the absence of cystidia, and amyloid, broadly ellipsoid basidiospores measuring 4.7–7 × 3.5–4.7 μm. Scytinostroma macrospermum is characterized by resupinate, coriaceous basidiomata with cream to straw yellow hymenophore, a dimitic hyphal structure with generative hyphae bearing simple septa, numerous cystidia embedded or projecting from hymenium, and inamyloid, ellipsoid basidiospores measuring 9–11 × 4.5–5.5 μm. The differences between the new species and morphologically similar and phylogenetically related species are discussed.

1 Introduction

The genus Scytinostroma Donk (Russulales, Basidiomycota), typified by S. portentosum (Berk. & M.A. Curtis) Donk, was established by Donk (1956). It is traditionally characterized by resupinate, coriaceous basidiomata, smooth to tuberculate hymenophore and a dimitic hyphal structure with simple septa or clamps on generative hyphae, filiform and dichotomously branched skeletal hyphae which are dextrinoid and cyanophilous, and subglobose to ellipsoid, thin-walled, variably amyloid basidiospores, and a white-rotting ecology (Donk, 1956; Rattan, 1974; Bernicchia and Gorjón, 2010; Wang et al., 2020; Tabish and Daniel, 2021).

The genus accommodated seven species derived from Corticium Fr. (without gloeocystidia) and Gloeocystidium P. Karst. (with gloeocystidia) when it was established. Later, Scytinostroma was gradually recognized by taxonomists, and the number of new species and new combinations has been increasing continuously (Donk, 1956; Gilbertson, 1962; Boidin, 1967; Rattan, 1974; Boidin and Lanquetin, 1977; Lanquetin, 1984; Boidin and Lanquetin, 1987; Boidin and Gilles, 1988; Hjortstam, 1990; Stalpers, 1996). So far, 36 species have been accepted in Scytinostroma worldwide (Nakasone, 2008; Liu, 2019; Wang et al., 2020). Recently, molecular phylogenetic studies demonstrated that Scytinostroma nested in Peniophoraceae within Russulales; furthermore, Scytinostroma was polyphyletic and formed four stable clades, as well as related to Gloiothele Bres., Vararia P. Karst., and Dichostereum Pilát (Nakasone and Micales, 1988; Larsson and Larsson, 2003; Miller et al., 2006; Larsson, 2007). Morphologically, Scytinostroma species are separated from other corticioid fungi of Russulales mainly by their tough and leathery texture of the basidiomata, as well as dextrinoid and dichotomously branched skeletal hyphae (Rattan, 1974; Liu, 2019).

During investigations on the diversity of wood-rotting fungi from China, two unknown corticioid specimens were collected from southwest China, and their morphology corresponded to the concepts of Scytinostroma. To confirm their affinity, phylogenetic analyses based on the ITS+ nLSU rDNA sequences were carried out. The two newly sequenced samples from Guizhou and Chongqing formed two well-supported lineages clustered with two sequences from Korea (KJ668461, Jang et al., 2016) and Japan (LC327052, Ogura-Tsujita et al., 2018), respectively. Based on morphological and phylogenetic evidences, we hereby propose two new species of Scytinostroma.

2 Materials and methods

2.1 Morphological studies

The studied specimens are deposited in the herbarium of the Institute of Microbiology, Beijing Forestry University (BJFC). Macro-morphological descriptions are based on field notes and dried specimens. Color terms followed Petersen (1996). Microscopic structures and abbreviations used in this study followed Wu et al. (2020) and Liu et al. (2022).

2.2 DNA extraction and sequencing

A CTAB rapid plant genome extraction kit (Aidlab Biotechnologies, Co., Ltd., Beijing, China) was used to obtain DNA products from voucher specimens, according to the manufacturer’s instructions with some modifications (Yuan et al., 2021; Yuan et al., 2022). The following primer pairs were used to amplify the DNA: ITS5 (5′-GGA AGT AAA AGT CGT AAC AAG G-3′) and ITS4 (5′-TCC TCC GCT TAT TGATAT GC-3′) for the internal transcribed spacer (ITS) regions (White et al., 1990); LR0R (5′-ACC CGC TGA ACT 6 TAA GC-3′) and LR7 (5′-TAC TAC CAC CAA GAT CT-3′) for nuclear large subunit (nLSU) rDNA (Vilgalys and Hester, 1990).

The procedures for DNA extraction and polymerase chain reaction (PCR) used in this study were the same as described by Wu et al. (2022b). The PCR products were purified and sequenced by Beijing Genomics Institute (BGI), China. All newly generated sequences in this study were deposited in GenBank (http://www.ncbi.nlm.nih.gov/genbank/) and listed in Table 1.

2.3 Phylogenetic analyses

Phylogenetic analyses were performed with the Maximum Parsimony (MP), Maximum Likelihood (ML), and Bayesian Inference (BI) methods. New sequences generated in this study, along with reference sequences retrieved from GenBank (Table 1), were aligned by MAFFT 7 (Katoh et al., 2019; http://mafft.cbrc.jp/alignment/server/) using the “G-INS-i” strategy and manually adjusted in BioEdit (Hall, 1999). Unreliably aligned sections were removed before the analyses, and efforts were made to manually inspect and improve the alignment. The data matrix was edited in Mesquite v3.70. Confertobasidium olivaceoalbum (Bourdot & Galzin) Jülich and Metulodontia nivea (P. Karst.) Parmasto were selected as outgroups (Larsson and Larsson, 2003).

MP topology and bootstrap (BT) values obtained from 1,000 replicates were computed in PAUP* version 4.0b10 (Swofford, 2002). All characters were equally weighted, and the gaps were treated as missing data. Trees were inferred using the heuristic search option with tree-bisection reconnection (TBR) branch swapping and 1,000 random sequence additions. Max-trees were set to 5,000, branches of zero length were collapsed, and all parsimonious trees were saved. Clade robustness was assessed by a BT analysis with 1,000 replicates (Felsenstein, 1985). Descriptive tree statistics, such as tree length (TL), consistency index (CI), retention index (RI), rescaled consistency index (RC), and homoplasy index (HI) were calculated for each Maximum Parsimonious Tree (MPT) generated.

RAxML 7.2.8 was used to construct ML trees for the combined dataset with the GTR+I+G model of site substitution, including estimation of Gamma-distributed rate heterogeneity and a proportion of invariant sites (Stamatakis, 2006). The branch support was evaluated with a bootstrapping method of 1000 replicates (Hillis and Bull, 1993).

The BI was conducted with MrBayes 3.2.6 in two independent runs, each of which had four chains for 5 million generations and started from random trees (Ronquist and Huelsenbeck, 2003). Trees were sampled every 1,000 generations. The first 25% of the sampled trees were discarded as burn-in, and the remaining ones were used to reconstruct a majority rule consensus and calculate Bayesian Posterior Probabilities (BPP) of the clades.

Branches that received BT supports for Maximum Parsimony (BP) and Maximum Likelihood (BS) greater than or equal to 75%, and BPP greater than or equal to 0.95 were considered as significantly supported. FigTree v1.4.4 and Treeview (Page, 1996) were used to visualize the resulting tree.

3 Results

3.1 Phylogenetic results

Two ITS and two nLSU sequences were generated in this study and were deposited in GenBank. Their accession numbers are specified in the phylogenetic tree (Figure 1). The final ITS + nLSU dataset included 60 sequences representing 28 species and resulted in an alignment of 1,826 characters. Maximum parsimony analysis yielded one equally parsimonious tree (TL = 2833, CI = 0.502, HI = 0.846, RI = 0.424, and RC = 0.498). BI analysis and ML analysis resulted in a similar topology to the MP analysis, with an average standard deviation of split frequencies of 0.002601 (BI).

Figure 1

The phylogeny (Figure 1) inferred from the ITS + nLSU dataset demonstrated that two new species, Scytinostroma acystidiatum and S. macrospermum, clustered in the Scytinostroma clade. Moreover, Scytinostroma acystidiatum clustered with one sample from Korea (KUC20121019-32) formed an independent lineage with a robust support (BP = 100%, BS = 100%, and BPP = 1.00) and then closely related to S. renisporum Boidin, Lanq. & Gilles. S. macrospermum clustered with one sample from Japan (M2138), forming an independent lineage with a strong support (BP = 100%, BS = 100%, and BPP = 1.00).

3.2 Taxonomy

Scytinostroma acystidiatum Q.Y. Zhang, L.S. Bian & Q. Chen, sp. nov., Figures 2, 3

Figure 2

Figure 3

MycoBank no.: 848524

Type — China, Guizhou Province, Tongren, Fanjingshan, on fallen angiosperm branch, 13 July 2022, Dai 24608 (BJFC039842).

Etymology—Acystidiatum (Lat.): refers to the species lacking cystidia.

Basidiomata —Annual, resupinate, coriaceous, not separable from substrate, up to 7 cm long, 2 cm wide, and less than 0.1 mm thick at center. Hymenial surface smooth to locally tuberculate, cream to pale yellow; margin concolorous with hymenial surface, thinning out, and adnate.

Hyphal structure —Hyphal system dimitic; generative hyphae infrequent, simple septate, hyaline, thin-walled, rarely branched, 2–3 μm in diameter, IKI–, CB–; skeletal hyphae dominant, frequently dichotomously branched, tortuous, interwoven, thick-walled, dextrinoid, cyanophilous, 1–2.5 μm in diameter; tissues unchanged in KOH.

Hymenium —Cystidia absent; cystidioles present, clavate, some gradually tapering to the apex, thin-walled, hyaline, smooth, 12–18 × 2–4 μm; basidia clavate, with a basal simple septum and four sterigmata, thin-walled, smooth, 13–21 × 3.5–5 μm; basidioles similar to basidia in shape, but slightly smaller.

Spores —Basidiospores broadly ellipsoid with an apiculus, hyaline, thin-walled, smooth, occasionally with one or two guttules, amyloid, acyanophilous, (4.5–)4.7–7 × (3–)3.5–4.7(–5) μm, L = 5.68 μm, W = 4.02 μm, Q = 1.41 (n = 30/1).

Scytinostroma macrospermum Q.Y. Zhang, L.S. Bian & Q. Chen, sp. nov., Figures 4, 5

Figure 4

Figure 5

MycoBank no.: 848525

Type —China, Chongqing, Jiangjin District, Simianshan National Scenic Spot, on rotten angiosperm wood, 10 July 2022, Dai 24606 (BJFC039838).

Etymology — Macrospermum (Lat.): refers to the species having large basidiospores.

Basidiomata —Annual, resupinate, coriaceous, not separable from substrate, up to 13.5 cm long, 3 cm wide, and less than 0.2 mm thick at center. Hymenial surface smooth to locally tuberculate, cream to straw yellow; margin concolorous with hymenial surface, slightly fimbricate.

Hyphal structure —Hyphal system dimitic; generative hyphae infrequent, simple septate, thin-walled, hyaline, rarely branched, 1.5–3 μm in diameter, IKI–, CB–; skeletal hyphae dominant, frequently dichotomously branched, interwoven, thick-walled, dextrinoid, cyanophilous, 1–3 μm in diameter; tissues unchanged in KOH.

Hymenium —Cystidia numerous, narrowly fusoid to cylindrical, thin-walled, smooth, 25–107 × 2.5–10 μm, embedded or projecting from hymenium up to 25 µm; basidia clavate, with a basal simple septum and four sterigmata, thin-walled, smooth, with some guttules, 30–45 × 6–8 μm; basidioles dominant, similar to basidia in shape, but slightly smaller.

Spores —Basidiospores ellipsoid with an apiculus, hyaline, thin-walled, smooth, occasionally with one or two guttules, inamyloid, acyanophilous, 9–11(–12) × (4–)4.5–5.5(–6) μm, L = 9.89 μm, W = 4.94 μm, Q = 2.00 (n = 30/1).

4 Discussion

Two new species, Scytinostroma acystidiatum and S. macrospermum, are described in this study based on morphological characteristics and phylogenetic analyses. The ITS + nLSU-based phylogeny (Figure 1) shows the phylogenetic positions of the two new species in the genus Scytinostroma. In detail, the sequence of KUC20121019-32 from Korea, clustered together with Scytinostroma acystidiatum, and shares less than 1.5% sequence (ITS) dissimilarity (Jang et al., 2016). The sample KUC20121019-32 was collected in Odaesan National Park, South Korea, which has geographical proximity (eastern Asia) and a similar climate (subtropical climate) to Guizhou, China. So, we treat KUC20121019-32 as Scytinostroma acystidiatum. In addition, Scytinostroma acystidiatum grouped with S. renisporum with strong support (100% BP, 100% BS, 1.00 BPP, Figure 1). Scytinostroma renisporum is morphologically distinguished from S. acystidiatum by its membranaceous to paper-like basidiomata and larger gloeocystidia measuring 20–35 × 6–10 µm (Boidin and Lanquetin, 1987).

Morphologically, Scytinostroma alutum Lanq., S. arachnoideum (Peck) Gilb., S. cystidiatum Boidin, S. hemidichophyticum Pouzar, S. portentosum (Berk. & M.A. Curtis) Donk, and S. yunnanense C.L. Zhao are similar to S. acystidiatum by sharing amyloid basidiospores. However, S. alutum differs from S. acystidiatum by its resupinate to effuse-reflexed basidiomata with cracked hymenophore, larger basidia (40–65 × 5–6 µm vs. 13–21 × 3.5–5 μm), and bigger basidiospores (5.3–7.2 × 5.7–7.3 μm vs. 4.7–7 × 3.5–4.7 μm; Lanquetin, 1984). Scytinostroma arachnoideum is separated from S. acystidiatum by its cottony basidiomata with white rhizomorphs and smaller basidiospores (3.5–4.5 × 3–3.5 μm vs. 4.7–7 × 3.5–4.7 μm; Gilbertson, 1962). Scytinostroma cystidiatum, S. hemidichophyticum, and S. portentosum are separated from S. acystidiatum by the presence of cystidia (Donk, 1956; Boidin, 1960; Pouzar, 1966). S. yunnanense differs from S. acystidiatum by its white to cream basidiomata and shorter basidiospores (4.5–5.5 μm vs. 4.7–7 μm in length; Wang et al., 2020).

Phylogenetically, the sequence of M2138 from Japan, clustered together with Scytinostroma macrospermum and formed an independent lineage with less than 1.5% sequence (ITS) dissimilarity (Ogura-Tsujita et al., 2018). The sample M2138 was collected in Kagoshima, Japan, which has geographical proximity (eastern Asia) and a similar climate (subtropical climate) to Chongqing, China. So, we treat M2138 as Scytinostroma macrospermum (Figure 1). Morphologically, Scytinostroma ochroleucum (Bres. & Torrend) Donk resembles S. macrospermum by resupinate, cream-colored to pale ochraceous basidiomata, but the former is different from the latter by its larger basidia (35–85 × 6.5–9 µm vs. 30–45 × 6–8 μm), and larger basidiospores (9–14 × 5–7 µm vs. 9–11 × 4.5–5.5 μm; Donk, 1956). Scytinostroma phaeosarcum Boidin & Lanq. resembles S. macrospermum by the approximately same size of basidiospores (8–10 × 4.5–5.5 μm), while S. phaeosarcum differs from S. macrospermum by its basidiomata becoming brown when bruised and thinner basidia (3–5 μm vs. 6–8 μm in width; Boidin and Lanquetin, 1977). In addition, Scytinostroma macrospermum is similar to S. decidens Boidin, Gilles & Lanq., S. jacksonii Boidin and S. mediterraneense Boidin & Lanq. by sharing large cystidia (> 100 μm in length) and inamyloid basidiospores. However, the latter three species distinctly differ from S. macrospermum by their obviously narrower basidiospores (2.5–3.5 μm in width vs. 4.5–5.5 μm in width, Boidin, 1981; Boidin and Lanquetin, 1987; Nakasone and Micales, 1988).

Wood-rotting fungi as an important group within the Basidiomycota are known for their ecological role in the forest ecosystem in terms of decaying living and dead trees and recycling nutrients in forest ecosystems (Dai et al., 2007; Yuan et al., 2021; Yuan et al., 2022). However, the diversity and taxonomy of these fungi remain not well known, and many new species have been described recently because of the application of molecular phylogeny (Dai et al., 2021; Mao et al., 2023; Wang et al., 2021; Wang et al., 2022; Wu et al., 2022a; Wu et al., 2022b; Zhou et al., 2021). Similarly, despite numerous species of Scytinostroma have been described, many unknown species or unnamed sequences still exist (Scytinostroma sp., Figure 1). Consequently, with the application of molecular phylogeny, the diversity and systematics will be outlined by further studies based on more samples worldwide.

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