AUTHOR=Su Renchun , Yuan Jinfeng , Gao Tianhui , Liu Yuhong , Shu Wei , Wang Yufeng , Pang Yu , Li Qi 

TITLE=Selection and validation of genes related to oxidative stress production and clearance in macrophages infected with Mycobacterium tuberculosis

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 13 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2023.1324611

DOI=10.3389/fcimb.2023.1324611

ISSN=2235-2988

ABSTRACT=Background: In the fight against tuberculosis, besides chemotherapy, the regulation of oxidative stress (OS) has also aroused people's interest in host-oriented therapy. However, there is limited research on the genes involved in reactive oxygen species (ROS) production and clearance in macrophages infected with Mycobacterium tuberculosis (MTB). This study analyzes and explores it to provide a basis for exploring new targets for anti-tuberculosis treatment. Methods: We established a macrophage model infected with MTB, counted intracellular bacteria, and determined the ROS using flow cytometry. We conducted ribonucleic acid sequencing, screened differential genes through transcriptomics methods, and validated them through reverse transcriptionquantitative polymerase chain reaction.The ROS of macrophages increased with intracellular bacteria at 4 hours after infection with MTB and reached its peak at 48 hours, surpassing the uninfected macrophages (p < 0.05). 1613 differentially expressed genes were identified after infection with MTB, of which 458 were associated with ROS, with over 50% involved in the response of organelles and biological processes to stimuli. We analyzed and identified six genes. After macrophage infection with MTB, the This is a provisional file, not the final typeset article expression of CAMK2B increased, while the expression of CYBB decreased (p < 0.05); The expression of GPX3 and SOD2 increased, while the expression of CAT decreased (p < 0.05).The ROS-related differentially expressed genes between MTB infected and uninfected macrophages may be related to some organelles and involved in various biological processes, molecular functions, and signaling pathways. Among them, CAMK2B, GPX3, and SOD2 may be related to ROS.