AUTHOR=Djusse Marielle Ezekielle , Prinelli Federica , Camboni Tania , Ceccarani Camilla , Consolandi Clarissa , Conti Silvia , Dall’Asta Margherita , Danesi Francesca , Laghi Luca , Curatolo Francesco Matteo , Morselli Sara , Foschi Claudio , Castellano Paola , Marangoni Antonella , Severgnini Marco 

TITLE=Dietary habits and vaginal environment: can a beneficial impact be expected?

JOURNAL=Frontiers in Cellular and Infection Microbiology

VOLUME=Volume 15 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/cellular-and-infection-microbiology/articles/10.3389/fcimb.2025.1582283

DOI=10.3389/fcimb.2025.1582283

ISSN=2235-2988

ABSTRACT=IntroductionIn reproductive-aged women, a vaginal microbiota dominated by several Lactobacillus species is crucial for maintaining vaginal health. Among the various factors affecting the composition of the vaginal ecosystem, the impact of dietary habits has rarely been explored. Thus, in this cross-sectional study, we assessed the role of macronutrient intake on the vaginal microbiota in a cohort of 113 young women, independently from potential confounders.MethodsFor each subject, we characterized (i) the vaginal bacterial community-state type (CST) by 16S rRNA gene profiling, (ii) the vagina lmetabolic profile by 1H-NMR spectroscopy, and (iii) the energy, nutrient and alcohol intake through a validated food frequency questionnaire.ResultsWe found that the increase in animal protein intake, mainly derived from red and processed meat, was positively associated with the dysbiotic condition of CST IV and, similarly, alcohol consumption was significantly associated with the levels of Gardnerella spp. and Ureaplasma spp. On the other hand, we noticed a beneficial effect of a-linolenic acid, with its increase inversely associated with CST III, dominated by the ‘less-protective’ species Lactobacillus iners. Moreover, linolenic acid was related to the abundance of Lactobacillus crispatus, in turn related tovarious vaginal metabolites such as 4-hydroxyphenyllactate and several amino acids. Total carbohydrates, vegetable proteins, total fiber, and starch were negatively correlated with Gardnerella spp.DiscussionWe highlighted that specific dietary habits (i.e., reduced consumption of alcohol and animal proteins, higher intake of linolenic acid) can have a beneficial impact on the vaginal environment, through the maintenance of a microbiota mainly dominated by ‘protective’ Lactobacillus species.