BRIEF RESEARCH REPORT article
Front. Cell. Infect. Microbiol.
Sec. Parasite and Host
This article is part of the Research TopicIn Situ Research in NTD-Endemic Regions: Science at the Frontlines of Global HealthView all articles
A Cross - sectional Investigation on the Molecular Infection Rate and Subtype Distribution of Blastocystis among Diarrhea Patients in Zhuhai, Southern Guangdong Province, China
Provisionally accepted
- 1Department of Clinical Laboratory, The Fifth Affiliated Hospital Sun Yat-sen University, Zhuhai, Zhuhai, China
- 2Wenzhou Medical University, Wenzhou, China
- 3Department of Infection Office, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China
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Introduction: Blastocystis sp. is a common zoonotic protozoan found in both humans and animals. Epidemiological investigations seldom concentrate on this parasite, frequently underestimating its medical significance. The current study determined the presence and genetic characteristics of Blastocystis sp. in diarrheal patients from Zhuhai City, China. Methods: A total of 283 fecal samples were collected from diarrheal patients for DNA extraction. Blastocystis subtypes (STs) were identified by analyzing the DNA sequences of the small ribosomal subunit of the parasite. Results: The overall infection rate of Blastocystis among the patients was 4.9% (14/283). The infection rate of Blastocystis was significantly higher in female patients (8.1%) than in male patients (2.9%); it was also higher in the elderly (6.3%) and adults (6.7%) compared to children (2.9%), although the difference was not statistically significant. Additionally, the infection rates of Blastocystis in rural (5.0%) and urban (4.9%) areas were consistent. Three known STs, including ST1 (n = 5), ST2 (n = 1) and ST3 (n = 8) were identified. A total of nine sequences were obtained across the three STs, including four known and five novel sequences. Conclusions: This study is the first to report on the distribution of Blastocystis STs among individuals with diarrhea in Zhuhai, China, revealing potential transmission of the parasite both from human-to-human and from animals to humans. Given the ongoing debate regarding the pathogenicity of Blastocystis, it is recommended that patients suffering from diarrhea be closely monitored to identify and address the underlying causes, thereby enhancing their health outcomes.
Keywords: Blastocystis, diarrheal patients, genetic, Guangdong, China, subtyping
Received: 26 Oct 2025; Accepted: 29 Nov 2025.
Copyright: © 2025 Lijie, Tan, Zhang, Guangxu, Huang and Zhao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Wei Zhao
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