AUTHOR=Young Stephanie Z., Platel Jean-Claude , Nielsen Jakob V., Jensen Niels A., Bordey Angelique 

TITLE=GABA<sub>A</sub> increases calcium in subventricular zone astrocyte-like cells through L- and T-type voltage-gated calcium channels

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=Volume 4 - 2010

YEAR=2010

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2010.00008

DOI=10.3389/fncel.2010.00008

ISSN=1662-5102

ABSTRACT=In the adult neurogenic subventricular zone (SVZ), the behavior of astrocyte-like cells and some of their functions depend on changes in intracellular Ca2+ levels and tonic GABA<sub>A</sub> receptor activation. However, it is unknown whether, and if so how, GABA<sub>A</sub> receptor activity regulates intracellular Ca2+ dynamics in SVZ astrocytes. To monitor Ca2+ activity selectively in astrocyte-like cells, we used two lines of transgenic mice expressing either GFP fused to a Gq-coupled receptor or DsRed under the human glial fibrillary acidic protein (hGFAP) promoter. GABA<sub>A</sub> receptor activation induced Ca2+ increases in 40-50% of SVZ astrocytes. GABA<sub>A</sub>-induced Ca2+ increases were prevented with nifedipine and mibefradil, blockers of L- and T-type voltage-gated calcium channels (VGCC). The L-type Ca2+ channel activator BayK 8644 increased the percentage of GABAA-responding astrocyte-like cells to 75%, suggesting that the majority of SVZ astrocytes express functional VGCCs. SVZ astrocytes also displayed spontaneous Ca2+ activity, the frequency of which was regulated by tonic GABA<sub>A</sub> receptor activation. These data support a role for ambient GABA in tonically regulating intracellular Ca2+ dynamics through GABA<sub>A </sub>receptors and VGCC in a subpopulation of astrocyte-like cells in the postnatal SVZ.