AUTHOR=Pascarella Giovanni , Lazarevic Dejan , Plessy Charles , Bertin Nicolas , Akalin Altuna , Vlachouli Christina , Simone Roberto , Faulkner Geoff , Zucchelli Silvia , Kawai Jun , Daub Carsten O., Hayashizaki Yoshihide , Lenhard Boris , Carninci Piero , Gustincich Stefano 

TITLE=NanoCAGE analysis of the mouse olfactory epithelium identifies the expression of vomeronasal receptors and of proximal LINE elements

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=Volume 8 - 2014

YEAR=2014

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2014.00041

DOI=10.3389/fncel.2014.00041

ISSN=1662-5102

ABSTRACT=By coupling laser capture microdissection to nanoCAGE technology and next-generation sequencing we have identified the genome-wide collection of active promoters in the mouse Main Olfactory Epithelium (MOE). Transcription start sites (TSSs) for the large majority of Olfactory Receptors (ORs) have been previously mapped increasing our understanding of their promoter architecture.<br/>Here we show that in our nanoCAGE libraries of the mouse MOE we detect a large number of tags mapped in loci hosting Type-1 and Type-2 Vomeronasal Receptors genes (V1Rs and V2Rs). These loci also show a massive expression of Long Interspersed Nuclear Elements (LINEs). We have validated the expression of selected receptors detected by nanoCAGE with in situ hybridization, RT-PCR and qRT-PCR. <br/>This work extends the repertory of receptors capable of sensing chemical signals in the MOE, suggesting intriguing interplays between MOE and VNO for pheromone processing. It positions transcribed LINEs as candidate regulatory RNAs for VRs expression.