Front. Cell. Neurosci. Frontiers in Cellular Neuroscience Front. Cell. Neurosci. 1662-5102 Frontiers Media S.A. 10.3389/fncel.2016.00107 Neuroscience Erratum Erratum: An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis Frontiers Production Office * Frontiers Production Office, Frontiers Switzerland

Approved by: Cellular Neuroscience Editorial Office, Switzerland

*Correspondence: Frontiers Production Office production.office@frontiersin.org

 25 04 2016 2016 10 107 11 04 2016 11 04 2016 Copyright © 2016 Frontiers Production Office. 2016 Frontiers Production Office

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

 An erratum on An Optimized Approach to Recover Secreted Proteins from Fibroblast Conditioned-Media for Secretomic Analysis by Paré, B., Deschênes, L. T., Pouliot, R., Dupré, N., and Gros-Louis, F. (2016). Front. Cell. Neurosci. 10:70. doi: 10.3389/fncel.2016.00070 secretomic 2D-DIGE secreted proteins secretome fibrolast-conditioned media

Reason for Erratum:

Due to a typesetting error, a misalignment in Table 2 lead to the publication of incorrect information. In the “Resolving gel” column, line “1.5M TRIS-HCl, pH 8.8,” the volume should be 4.5 mL, and not 4.5 L as published.

Detailed protocol for gel and electrophoresis experiments.

Stacking gel Resolving gel 10X Running Buffer Light gel Heavy gel Moving solution Conservation buffer DTT solution Iodoacetamine solution Equilibration buffer 5X running buffer Overlays solution
40% acrylamide/bisac rylamide (37.5:1) (w/v) 1 mL 6.3 mL
1.5M TRIS-HCl, pH 8.8 4.5 mL 52.5 mL 52.5 mL 50 mL 100 mL 50 mL
0.5M TRIS-HCl, pH 6.8 2.5 mL
10% SDS (w/v) 0.1 mL 0.18 mL 2.1 mL 2.1 mL 4 mL
10% APS 0.05 mL 0.09 mL 2.1 mL 1.05 mL
TEMED 0.015 mL 0.009 mL 360 μL 60 μL
Glycine 120 g
SDS 40 g 20 g 40 g
TRIS 576 g 60.5 g
30% acrylamide/bisac rylamide (37.5:1) (w/v) 68 mL 128 mL
Glycerol 18 mL 74 g 378 g
1% bromophenol blue 210 μL 20 μL
1M DTT 0.1 g
Iodoacetamine 1.12 g
Equilibration buffer 10 mL 25 mL
Urea 355 g
Agarose type I-A 0.4 g
Agarose type VII 0.1 g
5X running buffer 20 mL
Apyrogenic water 6.35 mL 6.93 mL to 4L 85 mL 14 mL 90 mL 296 mL To 1L to 4L 80 mL

Acrylamide/bisacrylamide (37.5 :1) solution (w/v) (BioBasic Inc, Markham, Ontario, Canada), agarose type I-A (Sigma, Oakville, Québec, Canada), ammonium persulfate (BioBasic Inc, Markham, Ontario, Canada), bromophenol blue (Sigma, Oakville, QC, Canada), dithiothreitol (DTT) (BioBasic Inc, Markham, Ontario, Canada), glycerol (BioBasic Inc, Markham, Ontario, Canada), glycine (BioBasic Inc, Markham, Ontario, Canada), iodoacetamide (BioBasic Inc, Markham, Ontario, Canada), sodium dodecyl sulfate (SDS) (BioBasic Inc, Markham, Ontario, Canada), TRIS (BioBasic Inc, Markham, Ontario, Canada), TRIS-HCl (BioBasic Inc, Markham, Ontario, Canada), tetramethylethylenediamine (TEMED) (BioBasic Inc, Markham, Ontario, Canada), urea (BioBasic Inc, Markham, Ontario, Canada), 2- hydroxythylagarose type VII (Sigma, Oakville, Québec, Canada).

The publisher apologizes for this error and the correct version of Table 2 appears below.

This error does not change the scientific conclusions of the article in any way.