AUTHOR=Lan Shihai , Zhou Lin , Wang Yimei , Fang Linchun , Yang Le , Zheng Suyue , Zhou XinHui , Tang Bin , Duan Jian , Wu Xiao , Yang Chengxing , Hong Tao 

TITLE=miRNA Profiling of Circulating Small Extracellular Vesicles From Subarachnoid Hemorrhage Rats Using Next-Generation Sequencing

JOURNAL=Frontiers in Cellular Neuroscience

VOLUME=Volume 14 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/cellular-neuroscience/articles/10.3389/fncel.2020.00242

DOI=10.3389/fncel.2020.00242

ISSN=1662-5102

ABSTRACT=Background: Extracellular vesicles (EVs) are produced during abnormal and normal physiological conditions. Understanding the expression profile of microRNA in plasma-derived small extracellular vesicles (sEVs) and their roles subarachnoid hemorrhage (SAH) that cause cerebral vasospasm (CVS) is imperative.
Methods: Sprague Dawley rats (250–300 g) were allocated to sham or SAH groups established using endovascular perforation method. miRNA expression profiles of plasma sEVs in both groups (each n = 4) were evaluated using next-generation sequencing (NGS).
Results: There were 142 miRNAs significantly expressed differently between the two groups, of which 73 were up-regulated while 69 were down-regulated in SAH sEVs compared with those of sham (P<0.05; fold change ≥2). Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Gene Ontology (GO) analyses of differently expressed (DE) miRNAs revealed signaling pathways and target genes (TGs) in SHA group. rno-miR-185-5p, rno-miR-103-3p, rno-miR-15b-3p, rno-miR-93-5p and rno-miR-98-5p were the top five most up regulated sEVs miRNAs.
Conclusion: Our results suggest that miRNA can be selectively packaged into sEVs under SAH, and this could help develop potential targets for prevention, diagnosis and treatment of CVS after this condition.