AUTHOR=Merlino Antonello 

TITLE=X-ray structures of metal-protein adducts at body temperature: concepts, examples and perspectives

JOURNAL=Frontiers in Chemical Biology

VOLUME=Volume 4 - 2025

YEAR=2025

URL=https://www.frontiersin.org/journals/chemical-biology/articles/10.3389/fchbi.2025.1670177

DOI=10.3389/fchbi.2025.1670177

ISSN=2813-530X

ABSTRACT=Most protein structures deposited in the Protein Data Bank have been determined at 100 K by collecting X-ray diffraction data while holding crystals under a liquid nitrogen flux. Recently, the growing awareness that low-temperature diffraction data collection is accompanied by artifacts and by a loss of physiologically relevant information has driven crystallographers to collect X-ray diffraction data at room temperature or at body temperature (37 °C). Here, the results obtained from recent structural determinations of metal-protein adducts at body temperature are briefly discussed.