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        <title>Frontiers in Chemical Engineering | Biochemical Engineering section | New and Recent Articles</title>
        <link>https://www.frontiersin.org/journals/chemical-engineering/sections/biochemical-engineering</link>
        <description>RSS Feed for Biochemical Engineering section in the Frontiers in Chemical Engineering journal | New and Recent Articles</description>
        <language>en-us</language>
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        <pubDate>2026-05-13T14:35:16.514+00:00</pubDate>
        <ttl>60</ttl>
        <item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2026.1792994</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2026.1792994</link>
        <title><![CDATA[Magnesium and bio-slurry enhance the properties of bio-tiles produced using an automated microbial calcium carbonate precipitation process]]></title>
        <pubdate>2026-04-30T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Emma Jane Horn</author><author>Denislav Marinov</author><author>Dyllon Garth Randall</author>
        <description><![CDATA[This study presents a novel, modular 3D printing system for the scalable production of microbially induced calcium carbonate (CaCO3) precipitation (MICP) produced bio-materials. Through modular design principles and operation automation, the technique offers a biochemical route to cementitious materials with the potential for lower energy and carbon footprints compared to traditional kiln-fired ceramics. The bio-tiles produced using this technique exceed international standards of breaking strength and modulus of rupture. By leveraging freeze-dried urease-active bio-slurry powder, the process ensures uniform bacterial distribution within the sand matrix, enhancing reproducibility and scalability. To establish a technical baseline for this automated platform, the effect of CaCO3 seed supplementation, magnesium-enriched cementation solution, and manual daily nutrient broth treatments was evaluated as a function of ureolytic activity and mineralization performance. Results revealed that magnesium addition paired with nutrient broth supplementation was a notable factor associated with the observed strength enhancement, achieving an average of 622 ± 84 N. While individual seed loadings showed variability consistent with gravity-driven infiltration, the aggregate performance of magnesium-supplemented tiles established a clear general technical trend, achieving strengths 3.3 times higher than bio-tiles treated without magnesium and only nutrient broth. However, nutrient broth treatments were found to be redundant as ureolytic requirements were likely satisfied by the bio-slurry itself. Additional CaCO3 seeds minimally influenced strength but improved edge solidification. Despite challenges in achieving uniform solidification without specific additives, the automated system successfully integrated submersion and pumping methodologies. The bio-tiles exceeded strength standards for tiles with a water absorption of greater than 10%, underscoring their potential for sustainable construction applications. This proof-of-concept highlights the mechanical automation as a key enabler for scalable MICP-based manufacturing, reducing labour intensity and optimising bio-cementation for sustainable, high-performance materials. Future research should focus on enhancing solidification consistency and advancing MICP-based bio-materials for structural applications. The integration of automation, modularity, and optimised MICP conditions marks a significant advancement toward the commercial-scale production of materials with improved environmental profiles.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2026.1791044</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2026.1791044</link>
        <title><![CDATA[Optimization of alkali-ethanol fractionation of Miscanthus using response surface methodology]]></title>
        <pubdate>2026-03-30T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Halim Choo</author><author>Niclas Conen</author><author>Moritz Doeker</author><author>Ralf Pude</author><author>Holger Klose</author><author>Andreas Jupke</author>
        <description><![CDATA[To address the demand for sustainable, sulfur-free fractionation, this study investigated the alkali-ethanol fractionation of Miscanthus × giganteus using sequential Response Surface Methodology (RSM) strategy to systematically quantify parameter interactions and optimize process performance. An initial Box-Behnken Design (BBD) was employed at low severity to screen four independent variables (temperature, time, alkali concentration and ethanol concentration), regarding their impact on pulp yield, pulp chemical composition, and xylan solubilization. Subsequently, a Central Composite Design (CCD) was used to optimize the principal process drivers (temperature and alkali concentration) at high severity. In both designs, distinct optima were identified for two specific objectives: maximizing polysaccharide yield and maximizing delignification. In the low severity regime, the predicted optimum for maximizing polysaccharide retention yielded a 73.3% pulp yield (96.9% cellulose and 92.2% xylan retention), while the delignification focused optimum achieved 83.1% lignin removal. In the high severity regime, maximizing polysaccharide yield resulted in 88.2% cellulose and 67.6% xylan retention, whereas delignification objective achieved 91.3% lignin removal. Chemical and morphological analyses confirmed that varying severity regimes and objectives produce pulps with distinct structural chemotypes. This study establishes a robust sequential RSM model that serves as a powerful tool for precisely tailoring non-wood feedstock fractionation, enabling biorefineries to switch between high yield intermediate pulps and high-purity cellulose streams based on specific market requirements.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2025.1629455</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2025.1629455</link>
        <title><![CDATA[-Omics approaches to study and model cell-cell interactions in engineered tissues]]></title>
        <pubdate>2025-08-28T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Christine Stark</author><author>Sarah E. Jenison</author><author>Mai T. Ngo</author>
        <description><![CDATA[-Omics technologies have emerged as powerful tools to uncover cellular diversity within tissues, and the role of cell-cell communication in tissue development, function, and disease. In this review, we will discuss recent advancements in -omics technologies that are used to interrogate the biomolecular mechanisms that underly tissue form and function. We will specifically discuss the application of -omics technologies, along with bioinformatic tools, towards identifying new cell types and cell-cell interactions within native tissues. We will then examine how insights from -omics technologies can inform the design of engineered tissues, particularly through the lens of recapitulating native cell-cell interactions. Finally, we will discuss how -omics can be employed to benchmark and analyze engineered tissues for applications that span fundamental science and translation. Overall, the integration of -omics and tissue engineering will improve our understanding of the roles of cellular diversity and cell-cell communication in regulating tissue health and disease and subsequently inform how cell-cell interactions can be leveraged to design therapies for human health applications.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2025.1637075</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2025.1637075</link>
        <title><![CDATA[Cell manufacturing for cell-based tissue engineering: a focus on vascularized, skeletal muscle regeneration]]></title>
        <pubdate>2025-08-07T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Bruno de Medeiros Cartaxo Esmeraldo</author><author>Keely A. Laurence</author><author>Brian J. Kwee</author>
        <description><![CDATA[Cell manufacturing processes play a crucial role in cell-based tissue engineering by isolating, purifying, culturing, expanding, modifying, cryopreserving, and formulating patient-derived cells in vitro before utilizing them for tissue regeneration. Currently, researchers apply various methods for cell manufacturing, including bioreactors, defined chemical cues, and substrate modifications. However, factors such as loss of cell potency and heterogeneity are critical challenges when engineering tissues for regenerative medicine. In particular, neglecting cellular heterogeneity during cell expansion prevents the formation of tissues that recapitulate the structural and cellular heterogeneity of our native tissues. This review discusses current and emerging approaches for cell manufacturing, with a focus on biomanufacturing for vascularized, skeletal muscle tissue engineering. Specifically, this review highlights 1) the U.S. Food and Drug Administration’s regulation of manufacturing for cell therapies, 2) state-of-the-art approaches for manufacturing endothelial cells and muscle stem cells that maintain cellular identity and potency, and 3) emerging tools and methods for measuring and manipulating cellular heterogeneities. Ultimately, these approaches can be leveraged to manufacture and formulate tissue-engineered products that mimic the heterogeneous form and function of our native tissues.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2025.1609277</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2025.1609277</link>
        <title><![CDATA[Aqueous two-phase separation enables selective purification of mono-PEGylated human serum albumin: influence of process parameters and reagent size]]></title>
        <pubdate>2025-08-06T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Salem Alkanaimsh</author><author>Osama A. Al-Rashed</author><author>Mohamed Shaaban</author>
        <description><![CDATA[PEGylation is widely used in biopharmaceuticals to enhance protein stability and half-life, but the resulting mixtures typically contain multiple PEGylated derivatives alongside unmodified proteins, complicating purification. In this study, we developed a novel aqueous two-phase separation (ATPS) strategy for selectively purifying mono-PEGylated human serum albumin (HSA). HSA was PEGylated using polyethylene glycol (PEG) reagents of different molecular weights (20 kDa and 40 kDa) and subsequently purified using ATPS. Our results demonstrated that ATPS effectively isolated PEGylated HSA with purity >99% and extremely high selectivity in the top phase. Tie-line length (TLL) significantly influenced yield and purity, whereas the volume ratio (Vr) had a minimal effect. Optimal conditions for the separation of 20 kDa PEGylated HSA were identified at a TLL of 29% (w/w) and a Vr of 2.5, achieving a yield of 50% and an equilibrium constant of 1.6. Under identical conditions, the yield and equilibrium constants for 40 kDa PEGylated HSA increased to 58% and 18, respectively, attributed to enhanced hydrophobic interactions from the larger PEG reagent. Furthermore, ATPS reached equilibrium rapidly within 30 min, resulting in high productivity levels of 1.3 and 1.5 g/L/h for 20 and 40 kDa PEGylated HSA, respectively. These findings illustrate the high efficiency and industrial potential of ATPS as an effective purification strategy for PEGylated therapeutic proteins.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2025.1593014</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2025.1593014</link>
        <title><![CDATA[Erratum: Effect of nitrogen sources on the yield and quality attributes of capsular polysaccharides in Streptococcus pneumoniae]]></title>
        <pubdate>2025-03-25T00:00:00Z</pubdate>
        <category>Erratum</category>
        <author>Frontiers Production Office </author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2025.1518165</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2025.1518165</link>
        <title><![CDATA[Effect of nitrogen sources on the yield and quality attributes of capsular polysaccharides in Streptococcus pneumoniae]]></title>
        <pubdate>2025-03-12T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yuelong Li</author><author>Yanli Liu</author><author>Hantian Yao</author><author>Yanyan Wang</author><author>Yechi Zhou</author><author>Hao Zheng</author><author>Yanbin Liu</author><author>Xinyan You</author><author>Xin Cao</author><author>Jiankai Liu</author>
        <description><![CDATA[Streptococcus pneumoniae, a pathogenic bacterium, is responsible for a range of infections. With the rise in antibiotic resistance, vaccination against pneumococcal disease has become increasingly critical. Pneumococcal capsular polysaccharides (CPSs) serve as potent vaccine antigens, triggering the host’s production of protective antibodies. The immunogenicity of CPS antigens in pneumococcal vaccines is significantly influenced by the chain length, the content of functional chemical groups and additional chemical modifications. S. pneumoniae has stringent nutritional requirements for culture medium. One crucial aspect of fermentation medium development is the selection of nitrogen sources. These sources supply the essential nutrients for the synthesis of vital biomolecules and secondary metabolites, including the CPSs. Therefore, comprehending the impact of organic nitrogen sources on the yield and quality of CPSs is crucial for optimizing manufacturing processes for pneumococcal vaccines. In our study, we evaluated the effects of peptones from various sources on the growth profiles and CPS yields, as well as quality attributes related to CPS immunogenicity. We found that while CPS productivity was slightly impacted by peptone selection, the chain length and functional group content of CPSs were markedly influenced by the peptone source. Notably, using the non-animal HY-SOY 4D soy peptone as a nitrogen source in the fermentation medium led to CPSs with long chains and a high content of functional chemical groups. The structural identity and correctness of pure CPSs were verified by 1H nuclear magnetic resonance (NMR) spectroscopy. The findings offer insights into how the composition of the fermentation medium affects both the yield and quality of pneumococcal CPSs, aiming at improving vaccine production against pneumococcal infections.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1514962</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1514962</link>
        <title><![CDATA[The role of Latin America medicinal plants in wound healing]]></title>
        <pubdate>2025-01-13T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Mariana Sánchez-Ramos</author><author>Andrea Ruiz-Betancourt</author><author>Stephany Abigail Tadeo-Cuenca</author><author>Angélica Román-Guerrero</author><author>María Crystal Columba-Palomares</author><author>Araceli Guerrero-Alonso</author><author>Antonio Bernabé-Antonio</author><author>Deyanira Ojeda-Ramírez</author><author>Francisco Cruz-Sosa</author>
        <description><![CDATA[Wound healing represents a global public health problem when it is not treated correctly, which can cause complications for the patient, such as functional loss of an organ, amputation, and even death. At a biological level, wound healing involves a complex mechanism in which the immune system and cellular biochemical cascades intervene in a coordinated manner, whose development occurs in stages such as inflammation, proliferation, and remodeling. Therefore, therapies have been developed to accelerate wound healing and have proven effective. However, factors such as diabetes mellitus limit the healing process because it causes alterations in microvascular dysfunction, as well as in the inflammatory response and greater oxidative stress. This is reflected in an abnormal healing process; therefore, the search for healing compounds has become an area of interest. In this regard, medicinal plants have been used for centuries to treat wounds in different cultures in the world. Hence, this review documents the main plant species used in Latin America due to its great biodiversity and numerous species that are potentially important for the development of new active healing compounds. In this review, 62 plant families with wound healing studies were found, highlighting Fabaceae, Asteraceae and Euphorbiaceae family. Additionally, 32 natural compounds with diverse structural nature were found, whose effects have been evaluated in in vivo and in vitro models, which are essential for studying the pathogenesis of the tissue repair mechanism, detecting new biomarkers, and evaluate new treatments. Currently, several models are used to study the wound healing process, including in silico, in vitro, and in vivo models. On the other hand, there is no appropriate model to determine the wound healing effect, and, in many cases, they are combined to provide sufficient scientific evidence. Therefore, this review demonstrates that Latin America is a potential region for research into sources of healing molecules. Nevertheless, other species are still being studied whose scientific findings allow generating viable alternatives for the solution of health problems associated with wound healing.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1470991</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1470991</link>
        <title><![CDATA[Novel sparging strategies to enhance dissolved carbon dioxide stripping in industrial scale stirred tank reactors]]></title>
        <pubdate>2024-11-15T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Nicolas Nickel</author><author>Jürgen Fitschen</author><author>Ingrid Haase</author><author>Maike Kuschel</author><author>Torsten W. Schulz</author><author>Thomas Wucherpfennig</author><author>Michael Schlüter</author>
        <description><![CDATA[Aerated stirred tank reactors are widely used in bio-process engineering and pharmaceutical industries. To supply the organisms with oxygen and control the pH value, oxygen is transferred from air bubbles into the liquid phase, and, at the same time, carbon dioxide is stripped from the liquid phase with the same gas bubbles. The volumetric mass transfer coefficients for oxygen and carbon dioxide are, therefore, of crucial importance for the design and scale-up of aerated stirred tank reactors. In this experimental work, the volumetric mass transfer coefficients for oxygen and carbon dioxide are investigated simultaneously to study their mutual influence. The mass transfer performance for oxygen and carbon dioxide is conducted in stirred tank reactors on the 3 L laboratory scale, 30 L pilot scale, and 15,000 L production scale. First, the influence of dissolved carbon dioxide on the oxygen mass transfer performance is investigated in a 30 L pilot scale stirred tank reactor. The results show that the volumetric mass transfer coefficient of oxygen is not affected by the concentration of dissolved carbon dioxide, but the total mass flux of oxygen decreases with increasing carbon dioxide concentration due to the decreasing partial pressure difference. With rising gassing rate and volumetric power input, both mass transfer coefficients for oxygen and carbon dioxide show the same increasing trend. Although this trend can also be observed when scaling down to the 3 L laboratory scale reactor, a significantly different effect must be considered for the scale-up to the 15,000 L industrial scale reactor. The limited absorption capacity for carbon dioxide of the gas bubbles during the long residence time in the industrial scale reactor is noticeable here, which is why the specific interfacial area is of negligible importance. This effect is used to develop a method for independent control of oxygen and carbon dioxide mass transfer performance on an industrial scale and to increase the mass transfer performance for carbon dioxide by up to 25%.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1481257</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1481257</link>
        <title><![CDATA[Optimization of manufacturing process for serotype 14 pneumococcal capsular polysaccharide]]></title>
        <pubdate>2024-10-23T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Yuelong Li</author><author>Yongxue Xu</author><author>Xin Cao</author><author>Yanyan Wang</author><author>Jianlong Wang</author><author>Yi Zhao</author><author>Hui Wang</author><author>Hantian Yao</author><author>Jiankai Liu</author>
        <description><![CDATA[Streptococcus pneumoniae is a pathogenic bacterium that causes infections such as pneumonia, meningitis, otitis media, and bacteremia. The prevention of pneumococcal disease by vaccination has become more urgent due to increased antibiotic resistance. Pneumococcal capsular polysaccharides (CPS) are effective vaccine antigens that stimulate the host to produce protective antibodies. S. pneumoniae serotype 14 is one of most prevalent types in Latin America and across the world. However, the yield of S. pneumoniae serotype 14 CPS from existing fermentation processes remains low and requires improvement. In this study, various aspects of the fermentation process were optimized to improve pneumococcal growth and polysaccharide productivity, including feed medium, cultivation gas environment, fermentation pH, and temperature. A simplified purification method was also developed to obtain pure CPS, including ultrafiltration, acid and ethanol precipitation, diafiltration, and lyophilization. These fermentation optimizations significantly enhanced the optical density of pneumococcal bacterial cultures and increased fermentation yields to 2.4–2.6 g/L—significantly higher than previously achieved. Furthermore, the test results of pure CPS could meet the requirements in the European Pharmacopoeia (11th edition). These optimizations provide valuable insights into the nutritional requirements and impact of varying fermentation process parameters on pneumococcal growth and CPS productivity, thus contributing to the development of a more efficient and cost-effective method for the production of pneumococcal CPS—essential for manufacturing vaccines against pneumococcal infections.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1479798</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1479798</link>
        <title><![CDATA[The development and evaluation of chitosan-coated enzyme magnetic nanoparticles for cellulose hydrolysis]]></title>
        <pubdate>2024-10-03T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Heejoon Park</author><author>Patrick A. Johnson</author>
        <description><![CDATA[The recycling capability, colloidal and thermal stability of exo-cellulase, endo-cellulase, and β-glucosidases with magnetic particles (MNPs) were evaluated. Co-precipitation and oxidation of Fe(OH)2 methods were used to fabricate magnetic nanoparticles. Three different enzymes were covalently bound to the surface of MNPs using 3-(aminopropyl) triethoxysilane (APTES) and a common protein crosslinking agent, glutaraldehyde. To evaluate the increase in colloidal dispersion stability, chitosan-coating was applied on MNPs and evaluated through particle settlement tests. The results showed that the chitosan-coated MNPs had 3.7 times higher colloidal dispersion stability than the bare MNPs. X-ray photoelectron spectroscopy (XPS) confirmed each magnetic nanoparticle surface modification step and successful enzyme binding. The optimum bioconjugate ratio in exo-cellulase, endo-cellulase, and β-glucosidases was evaluated, and having a high endo-cellulase bioconjugate in the reaction produced the highest glucose. The bioconjugates showed superior glucose productivity 39.4% at 65°C and 22.2% at 88°C in which the native enzyme is inactivated completely after 5 h of exposure. Recycling stability studies showed approximately 78% of activity was retained after 10 cycles and 32% of activity was retained after 20 cycles. The bioconjugates demonstrated equivalent total product conversions as a single reaction of an equivalent amount of the native enzyme after the 10th cycle this work introduces a novel method for covalently binding individual exo-cellulase, endo-cellulase, and β-glucosidases. These bioconjugates showed superior thermal stability and recyclability. It was also demonstrated that chitosan coating significantly improves the colloidal dispersion stability of bioconjugates. Thus this work validates the use of enzyme-MNP bioconjugates to effectively glucose production and promising technique for eventual continuous biological processes.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1446454</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1446454</link>
        <title><![CDATA[Prospects for cordycepin biosynthesis in microbial cell factories]]></title>
        <pubdate>2024-07-30T00:00:00Z</pubdate>
        <category>Mini Review</category>
        <author>Xiang Li</author><author>Rongshuai Jiang</author><author>Shenghou Wang</author><author>Chenyang Li</author><author>Yongping Xu</author><author>Shuying Li</author><author>Qian Li</author><author>Liang Wang</author>
        <description><![CDATA[Cordycepin, an adenosine analog, exhibits diverse bioactivities and holds significant potential for applications in healthcare and agriculture. Fungi of the genus Cordyceps, such as Cordyceps militaris, can naturally produce cordycepin. Current sources of cordycepin primarily involve extraction from fruiting bodies or isolation from liquid fermentation using C. militaris, presenting challenges such as low production intensity, complex separation and purification systems, and high production costs, limiting industrial feasibility. Recent advancements have witnessed the utilization of various fungal chassis cells to successfully engineer heterologous biosynthetic platforms for cordycepin, such as Saccharomyces cerevisiae and unconventional yeasts, offering advantages of high yield, short fermentation cycles, and a broad substrate spectrum. This mini review summarizes the biosynthetic pathways of cordycepin and focused on the comparison of the characteristics, advantages, current performance and prospects for the microbial cell factories, analyzing potential targets for metabolic pathway modification and giving strategies in both genetic engineering and process engineering to enhance production intensity. The mini review particularly emphasizes the crucial role of chassis cell stress tolerance to the toxic product in determining cordycepin yield and highlights the urgent need for high-throughput screening methods for high-yield strains.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2024.1376921</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2024.1376921</link>
        <title><![CDATA[Editorial: 3D-engineered organoids for modelling tissue development and precision medicine]]></title>
        <pubdate>2024-02-09T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Federica Michielin</author><author>Luca Urbani</author><author>Camilla Luni</author><author>Onelia Gagliano</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1255783</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1255783</link>
        <title><![CDATA[Leveraging mechanobiology and biophysical cues in lung organoids for studying lung development and disease]]></title>
        <pubdate>2023-10-24T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Ziming Shao</author><author>Paolo De Coppi</author><author>Federica Michielin</author>
        <description><![CDATA[Lung organoids have emerged as powerful tools for studying lung distal diseases by recapitulating the cellular diversity and microenvironment of the lung tissue. This review article highlights the advancements in leveraging mechanobiology and biophysical cues in lung organoid engineering to improve their physiological relevance and disease modelling capabilities. We discuss the role of mechanobiology in lung development and homeostasis, as well as the integration of biophysical cues in the design and culture of lung organoids. Furthermore, we explore how these advancements have contributed to the understanding of lung distal diseases pathogenesis. We also discuss the challenges and future directions in harnessing mechanobiology and biophysical cues in lung organoid research. This review showcases the potential of lung organoids as a platform to investigate the underappreciated impacts of biophysical and biomechanical properties in enhancing lung organoids complexity and functionality, and ultimately provide new insight into embryonic lung development and pulmonary distal diseases pathogenesis.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1256334</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1256334</link>
        <title><![CDATA[Human gastro-intestinal organoid engineering: a state of the art]]></title>
        <pubdate>2023-10-16T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Giada Benedetti</author><author>Beatriz Fournon Berodia</author><author>Paolo De Coppi</author><author>Giovanni Giuseppe Giobbe</author>
        <description><![CDATA[Gastrointestinal organ failure, from congenital or postnatally acquired pathologies, is a major cause of death across countries of all income levels. Organoids and engineered tissues have been widely investigated as tools to model organ functions and treat pathologies. In this review we aim to describe the progress in human organoid engineering applied to the gastrointestinal tract (namely esophagus, stomach, and intestine). Starting from the onset of the organoid culture technique, we illustrate genetic engineering, stem cell niche engineering, bioprinting, and microfluidics approaches used to integrate mechano-physiological parameters with human organoids. Thanks to these improvements, organoid technology allows disease modelling of patient-specific pathologies, and personalized treatment screening, also offering a cell source for autologous transplantation. We further present an overview of the advances of tissue engineering in animal systems, concerning novel materials and scaffolds to be combined with a variety of cell types to reconstitute a viable surrogate for implantation. The effort in this field sets organoids as an important tool in personalized and regenerative medicine. Their application combined with the advances in tissue engineering holds great potential for translational application.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1267210</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1267210</link>
        <title><![CDATA[Editorial: Saccharomyces cerevisiae as a model organism for biochemical engineering and bioprocesses]]></title>
        <pubdate>2023-08-17T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Vasudeo Zambare</author><author>Mohd Fadhil Md Din</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1266904</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1266904</link>
        <title><![CDATA[Editorial: Biochemical/biomaterial production from lignocellulosic biomass]]></title>
        <pubdate>2023-08-02T00:00:00Z</pubdate>
        <category>Editorial</category>
        <author>Jie Dong</author><author>Daehwan Kim</author><author>Chang Geun Yoo</author>
        <description></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1182817</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1182817</link>
        <title><![CDATA[Deep neural network for prediction and control of permeability decline in single pass tangential flow ultrafiltration in continuous processing of monoclonal antibodies]]></title>
        <pubdate>2023-07-20T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Naveen G. Jesubalan</author><author>Garima Thakur</author><author>Anurag S. Rathore</author>
        <description><![CDATA[Single-pass tangential flow filtration (SPTFF) is a crucial technology enabling the continuous manufacturing of monoclonal antibodies (mAbs). By significantly increasing the membrane area utilized in the process, SPTFF allows the mAb process stream to be concentrated up to the desired final target in a single pass across the membrane surface without the need for recirculation. However, a key challenge in SPTFF is compensating for flux decline across the membrane due to concentration polarization and surface fouling phenomena. In continuous downstream processing, flux decline immediately impacts the continuous process flowrates. It reduces the concentration factor achievable in a single pass, thereby reducing the final concentration attained at the outlet of the SPTFF module. In this work, we develop a deep neural network model to predict the NWP in real-time without the need to conduct actual NWP measurements. The developed model incorporates process parameters such as pressure and feed concentrations through inline sensors and a spectroscopy-coupled data model (NIR-PLS model). The model determines the optimal timing for membrane cleaning steps when the normalized water permeability (NWP) falls below 60%. Using SCADA and PLC, a distributed control system was developed to integrate the monitoring sensors and control elements, such as the NIRS sensor for concentration monitoring, the DNN model for NWP prediction, weighing balances, pressure sensors, pumps, and valves. The model was tested in real-time, and the NWP was predicted within <5% error in three independent test cases, successfully enabling control of the SPTFF step in line with the Quality by Design paradigm.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1193230</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1193230</link>
        <title><![CDATA[Improvement of Saccharomyces propagation performance through oxygen-enriched air and aeration parameter variation]]></title>
        <pubdate>2023-05-18T00:00:00Z</pubdate>
        <category>Original Research</category>
        <author>Alexander Beugholt</author><author>Dominik Ulrich Geier</author><author>Thomas Becker</author>
        <description><![CDATA[A variety of yeast applications in the food and beverage industry require individual and reproducible yeast propagation at high yields and consistent quality. One quality-determining parameter for yeast propagation is effective aeration to avoid oxygen depletion. Therefore, this work investigated three important aeration parameters: airflow, pulse time, and oxygen concentration, for their influence on yeast propagation. The aeration of a propagator involves phase transitions which are gradient-driven processes and can be accelerated with higher gradients between the liquid medium and the gas bubbles. In this study, oxygen-enriched air generated with membrane filters was used to aerate the system in an easy and cost-efficient way without the need for expensive technical gas usage. Propagation experiments were carried out in a pilot-scale reactor equipped with a membrane filter system for enhanced oxygen concentrations in ingas and online sensors for representative monitoring of the process. The membrane filter system is based on the separation of nitrogen in compressed air, leading to oxygen enrichment. Using oxygen-enriched air for propagation aeration showed higher oxygen transfer into the medium and the anaerobic process time caused by oxygen depletion due to high cell numbers was reduced by an average of 7.4% for pulsed aeration. Additionally, we conducted experiments with controlled measures of dissolved oxygen using different oxygen concentrations for aeration. The main objective of this study is to present a new and affordable optimization of propagation aeration using membrane filtration to enrich process air. The results showed increased cell counts for higher ingas oxygen concentrations and no negative impact on cell vitality was observed. Hence, our investigations showed that using oxygen-enriched air reduced the frequency of pulsed aeration, thus hindering foam formation, a limiting factor of the yeast propagation process.]]></description>
      </item><item>
        <guid isPermaLink="true">https://www.frontiersin.org/articles/10.3389/fceng.2023.1120348</guid>
        <link>https://www.frontiersin.org/articles/10.3389/fceng.2023.1120348</link>
        <title><![CDATA[Organoids in high-throughput and high-content screenings]]></title>
        <pubdate>2023-03-21T00:00:00Z</pubdate>
        <category>Review</category>
        <author>Franziska L. Lampart</author><author>Dagmar Iber</author><author>Nikolaos Doumpas</author>
        <description><![CDATA[Organoids are self-organized three-dimensional (3D) multicellular tissue cultures which derive from cancerous and healthy stem cells, sharing a highly similarity to the corresponding in vivo organs. Since their introduction in 2009, they have emerged as a valuable model for studying early embryogenesis, organ and tissue development, as well as tools in drug screening, disease modeling and personalized therapy. Organoids can now be established for various tissues, including brain, retina, thyroid, gastrointestinal, lung, liver, pancreas, and kidney. These micro-tissues resemble the native organ in terms of gene expression, protein expression, tissue architecture and cell-cell interactions. Despite the success of organoid-based research and the advances in patient-derived organoid culture, important challenges remain. In this review, we briefly showcase the evolution from the primary 3D systems to complex, multilayered 3D structures such as assembloids, gastruloids and ETiX embryoids. We discuss current developments in organoid research and highlight developments in organoid culturing systems and analysis tools which make organoids accessible for high-throughput and high-content screening. Finally, we summarize the potential of machine learning and computational modeling in conjunction with organoid systems.]]></description>
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