AUTHOR=Zhang Nan , Wei Shuxiang , Cao Shijie , Zhang Qiang , Kang Ning , Ding Liqin , Qiu Feng 

TITLE=Bioactive Triterpenoid Saponins From the Seeds of Aesculus chinensis Bge. var. chekiangensis

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 7 - 2019

YEAR=2020

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2019.00908

DOI=10.3389/fchem.2019.00908

ISSN=2296-2646

ABSTRACT=Phytochemical investigation of Aesculus chinensis Bge. var. chekiangensis (Hu et Fang) Fang obtained 33 triterpenoid saponins, including fourteen new ones, Aesculiside C -P (1-14). The structure elucidations were performed through comprehensive MS, 1D and 2D-NMR analysis, and their absolute configuration were unambiguously determined by X-ray diffraction analysis as well as Mo 2 (OAc) 4 -induced ECD method for the first time. All the substances were examined for their cytotoxic activities against three tumor cell lines, Hep G2, HCT-116 and MGC-803. Of these, compounds 8, 9, 14-16, 18, 22 exhibited potent cytotoxicities against all cell lines with IC 50 of 1.77 to 20.51 μM, while compounds 3, 6, 7, 17-19, 20, 24, 28 dipicted moderate activity (IC 50 12.92 to > 40 μM). On these basis, the preliminary structure-activity correlations were also discussed. Meanwhile the neuroprotective properties of triterpenoid saponins from Aesculus genus were evaluated for the first time. Among them, compounds 1, 4, 12, 20, 22, 25, 29, 31 exhibited moderate activities against C O Cl 2 -induced PC12 cell injury.