AUTHOR=Mujeeb Anzar Abdul , Khan Nuha Abeer , Jamal Fauzia , Badre Alam Khan Farheen , Saeed Haris , Kazmi Shadab , Alshameri Ansam Wadia Faid , Kashif Mohammad , Ghazi Irfan , Owais Mohammad 

TITLE=Olax scandens Mediated Biogenic Synthesis of Ag-Cu Nanocomposites: Potential Against Inhibition of Drug-Resistant Microbes

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2020.00103

DOI=10.3389/fchem.2020.00103

ISSN=2296-2646

ABSTRACT=In the present study, we have synthesized Silver-Copper nanocomposites (Ag-Cu NCs) using Olax scandens leaf extract (green synthesis method) and evaluated their antimicrobial potential against less susceptible pathogens. The kinetics of  Ag-Cu NCs synthesis was followed by UV-VIS and fluorescence spectroscopy. The physicochemical characterization of as-synthesized  Ag-Cu NCs was executed using electron microscopy, Energy Dispersive X-Ray, Fourier Transform Infrared Spectroscopy and Differential Light Scattering method. As-synthesized  Ag-Cu NCs induce formation of Reactive Oxygen Species (ROS), thereby causing alteration and decrementation of cellular proteins, DNA, lipids etc., and eventually leading to cell death as determined Live/Dead assay. Next, we assessed anti-biofilm potential of as-synthesized  Ag-Cu NCs against biofilm forming bacteria. The as-synthesized  Ag-Cu NCs, when compared to monometallic silver nanoparticles, exhibit significantly higher anti-microbial activity against both sensitive as well as drug resistant microbial isolates.