AUTHOR=Baek Seung Hee , Song Hyun Woo , Lee Sunwoong , Kim Jung-Eun , Kim Yeo Hyang , Wi Jung-Sub , Ok Jong G. , Park Jun Seok , Hong Seonki , Kwak Moon Kyu , Lee Hye Jin , Nam Sung-Wook 

TITLE=Gold Nanoparticle-Enhanced and Roll-to-Roll Nanoimprinted LSPR Platform for Detecting Interleukin-10

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 8 - 2020

YEAR=2020

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2020.00285

DOI=10.3389/fchem.2020.00285

ISSN=2296-2646

ABSTRACT=Localized surface plasmon resonance (LSPR) is a powerful platform for detecting biomolecules including protein, nucleotide, and vesicle. Here, we report a colloidal Au nanoparticle-based assay to enhance the LSPR signal of a nanoimprinted Au strip structure. The induced colloidal Au nanoparticle leads a red-shift of the LSPR extinction peak, enabling us to detect IL-10 cytokines on the Au strip platform. For LSPR sensor fabrication, we employed a roll-to-roll nanoimprint process to create nanograting structures on polyethylene (PET) film. By angled deposition of Au upon the PET film, we demonstrated double-bent Au structure which has a strong LSPR extinction peak at ~760 nm. Upon the Au LSPR sensor, we performed an enzyme-linked immunosorbent assay (ELISA) protocol by forming a sandwich structure such as IL-10 capture antibody/IL-10/IL-10 detection antibody. To enhance the LSPR signal, we introduced a colloidal Au nanocube (AuNC) which is cross-linked to the IL-10 detection antibody of immunogold assay.  We detected IL-10 cytokine as a model protein at nanomolar range by demonstrating up to 450 % signal enhancement of the extinction peak shift. We expect that the AuNC-assisted LSPR sensor platform can be utilized as one of the diagnostic tools by offering convenient and fast detection of LSPR signal.