AUTHOR=Tawre Madhumita S. , Shiledar Aishwarya , Satpute Surekha K. , Ahire Kedar , Ghosh Sougata , Pardesi Karishma TITLE=Synergistic and antibiofilm potential of Curcuma aromatica derived silver nanoparticles in combination with antibiotics against multidrug-resistant pathogens JOURNAL=Frontiers in Chemistry VOLUME=Volume 10 - 2022 YEAR=2022 URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1029056 DOI=10.3389/fchem.2022.1029056 ISSN=2296-2646 ABSTRACT=Abstract: Hospital acquired infections caused due to ESKAPE pathogens pose a challenge for treatment due to their growing antimicrobial resistance. Curcuma aromatica (CA) is traditionally known for its antibacterial and anti-inflammatory properties. The present study highlights the biogenic synthesis of silver nanoparticles (CAAgNPs) capped and stabilized by the compounds from CA extract, also further demonstrating their antibacterial, antibiofilm and synergistic effects against MDR pathogens. CAAgNPs were synthesized using rhizome extract of CA (0.5 mg/mL) and AgNO3 (0.8 mM) incubated at 60°C up to 144 h. UV-vis spectroscopy, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDS) and X-ray diffraction (XRD) revealed CAAgNPs with characteristic peak at 430 nm, 13±5 nm spherical shape, showing presence of silver and crystalline nature. Dynamic light scattering and zeta potential confirmed their monodispersed (10-200 nm) nature and stability. Fourier transform infrared spectroscopic (FTIR) analysis demonstrated the presence of phenolic -OH and carbonyl groups possibly involved in the reduction and stabilization of CAAgNPs. The minimum inhibitory (MIC) and biofilm inhibitory (MBIC) concentrations of CAAgNPs against Pseudomonas aeruginosa, NCIM 5029 and PAW1, and, Staphylococcus aureus, NCIM 5021 and S8 were 8-64 µg/mL. Almost 50% disruption of pre-formed biofilms at concentrations 8-1024 µg/mL was observed. FESEM analysis confirmed disruption of preformed biofilms of P. aeruginosa PAW1 and S. aureus S8. Checkerboard assay demonstrated the synergistic effect of CAAgNPs (0.125-4 µg/mL) in combination with various antibiotics (0.063-1024 µg/mL) against planktonic and biofilm forms of P. aeruginosa PAW1. The study confirms the antibacterial and antibiofilm activity of CAAgNPs alone and in combination with antibiotics against MDR pathogens, thus, reducing the dose as well as toxicity of both. CAAgNPs have the potential to be used in wound dressings and ointments, and to improve the performances of medical devices and surgical implants. In vivo toxicity of CAAgNPs however needs to be tested further using mice models.