AUTHOR=Meetani Mohammed A. , Alhalabi Ahmad , Al-Tabaji Mohammed K. , Al-Hemyari Abdulla , Saadeh Haythem A. , Saleh Na’il 

TITLE=Cucurbituril—assisted sensitive fluorescence detection and quantitation of naproxen drug in wastewater samples: Guest-host characterization and HPLC investigation

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1093231

DOI=10.3389/fchem.2022.1093231

ISSN=2296-2646

ABSTRACT=Fluorescence spectroscopy, mass spectrometry, 1H-NMR, and liquid chromatography with fluorescence detection were used to investigate the guest-host interaction of naproxen drug and cucurbiturils.  Naproxen was found to form a supramolecular complex with cucurbit [7] uril (CB7) with a high formation constant. Specrofluorometry was used to investigate the optimal conditions for the interaction, which were found to be 0.2 mg/mL of CB7, 2.4 µg/mL of naproxen drug, and pH10. Proton NMR and tandem mass spectrometry reveal a 1:1 complex between naproxen and CB7. The HPLC with fluorescence detector was used to detect naproxen in influent and effluent wastewater samples using the standard addition calibration method.  Finally, it was found that after sample enrichment of 1000 mL into 1 mL, the determined levels of naproxen in the influent and the effluent wastewater were 1.87 x 10-4 ppb and 2.1 x 10-5 ppb, respectively.