AUTHOR=Jing Pengwei , Wang Ying , Sun Weixue , Li Guishi , Zhang Zuofu , Xu Qiang , Li Hao 

TITLE=A biocatalytic peptidobiosensing molecular bridge for detecting osteosarcoma marker protein

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 10 - 2022

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.1112111

DOI=10.3389/fchem.2022.1112111

ISSN=2296-2646

ABSTRACT=A biosensing scheme requiring only one-step sample incubation before signal collection, and using a compact “three-in-one” probe of target-binding, signal conversion, and amplification, may greatly simplify the design of biosensors. Therefore, sparing the multi-step addition of enzymes, protein, and nanomaterial, as well as the associated complexity and non-specific interactions. In this work, a peptide probe aimed at such compact features has been designed, based on protein-triggered, conformation-driven, and Cu (II) facilitated side-chain di-tyrosine cyclization. This design can use target-probe recognition to induce discriminated cross-linking and self-cleavage of the probe in the presence/absence of the target protein, resulting in retention or dissociation of a signal amplification motif from the search and consequently quantitative detection performance. The method has also been tested preliminarily in fractioned osteosarcoma clinical samples, showing an acceptable coherence between signal readout and clinical development of cancer. Based on these preliminary results, it may be expected that the proposed probe may be helpful for the future development of tumor screening and prognosis sensors.