AUTHOR=Feng Beidou , Wang Kui , Wang Zhe , Niu Huiyu , Wang Ge , Chen Yuehua , Zhang Hua 

TITLE=Mitochondrial-Targeted Ratiometric Fluorescent Probe to Monitor ClO− Induced by Ferroptosis in Living Cells

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.909670

DOI=10.3389/fchem.2022.909670

ISSN=2296-2646

ABSTRACT=Ferroptosis is a type of iron-dependent programmed cell death. Once such kind of death occurs, an individual cell would appear a series of changes related to reactive oxygen species (ROS) in mitochondria. Herein, a mitochondrial-targeted ratiometric fluorescent probe (MBI-OMe) was developed to specifically detect ferroptosis-induced ClO-, whose recognition group is p-methoxyphenol and mitochondrial-targeted group is benzimidazole. The fluorescence of MBI-OMe was firstly quenched by 30 μM of Fe3+. And then, MBI-OMe appeared a ratiometric signal at 477 nm and 392 nm to ferroptosis-induced ClO- in living cells. MBI-OMe was successfully used to evaluate changes in ClO- induced by ferroptosis.