AUTHOR=Bowen Hou , Durrani Rabia , Delavault André , Durand Erwann , Chenyu Jiang , Yiyang Long , Lili Song , Jian Song , Weiwei Huan , Fei Gao 

TITLE=Application of deep eutectic solvents in protein extraction and purification

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 10 - 2022

YEAR=2022

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2022.912411

DOI=10.3389/fchem.2022.912411

ISSN=2296-2646

ABSTRACT=Deep Eutectic Solvents (DESs) are a mixture of Hydrogen Bond Donor (HBD) and Hydrogen Bond Acceptor (HBA) species that can consist respectively of natural plant metabolites such as sugars, carboxylic acids, amino acids and ionic molecules which are for the vast majority ammonium salts. Media such as DESs are modular tools of sustainability that can be pointed towards the extraction of bioactive molecules due to their excellent physicochemical properties, their relatively low price and accessibility. The present review focuses on the application of DESs for protein extraction and purification. The in-depth effects and principles that apply to DES-mediated extraction using various renewable biomasses will be discussed as well. One of the most important observations made being that DESs have a clear ability to maintain the biological and/or functional activity of the extracted proteins, as well as increasing their stability compared to traditional solvents. They demonstrate true potential for a reproducible but more importantly, scalable protein extraction and purification compared to traditional methods while enabling waste valorization in some particular cases. 

Keywords: Deep eutectic solvent, Protein, Extraction, Green solvent