AUTHOR=Xu Xianjun , Wen Jiake , Wang Shuangqi , Hao Jia , Du Kunze , Fang Shiming , He Jun , Li Jin , Chang Yanxu 

TITLE=Simultaneous extraction and determination of alkaloids and organic acids in Uncariae Ramulas Cum Unicis by vortex-assisted matrix solid phase dispersion extraction coupled with UHPLC-MS/MS

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1100150

DOI=10.3389/fchem.2023.1100150

ISSN=2296-2646

ABSTRACT=A simple and efficient vortex-assisted matrix solid phase dispersion with ultra-high-performance liquid chromatography-triple quadrupole mass spectrometer (VA-MSPD-UHPLC-MS/MS) was applied for simultaneous extraction and determination the seven alkaloids and three organic acids from Uncariae Ramulas Cum Unicis. The optimal extraction conditions of the targets components were obtained by Box-Behnken design (BBD) combined with response surface methodology (RSM) experiment. The results of the method validation showed that this analytical method displayed a good linearity with the correlation coefficients (r) no lower than 0.9990. The recoveries of ten active ingredients from Uncariae Ramulas Cum Unicis ranged from 95.9%-103% (RSD ≤ 2.77%). The RSDs of intra-day and inter-day precision were all below 2.97%. The present method possessed not only lower solvent and sample usage, but also shorter sample processing and analysis time. Consequently, the developed VA-MSPD-UHPLC-MS/MS method could be used successfully and effectively for the extraction and analysis of the ten active components from Uncariae Ramulas Cum Unicis.