AUTHOR=Xia Dongfang , Zhang Yong , Zhang Chunyu , Yao Xiuxiu , Tang Yuhua , Wang Fuchao , Han Xu , Yin Hongzong , Xu Chao , Gao Xueyun 

TITLE=Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1145415

DOI=10.3389/fchem.2023.1145415

ISSN=2296-2646

ABSTRACT=The αvβ3 is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion and metastasis. Therefore, it is of great significance to precisely detect αvβ3 level in cells in simple way. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to its bright fluorescence, well-defined Pt atom numbers and peroxidase-like catalytic activity, this cluster can be used to evaluate αvβ3 levels in cells by fluorescence imaging, inductively coupled plasma mass spectrometry (ICP-MS) and catalytic amplification of visual dye, respectively. In this report, the expression level of αvβ3 in living cells are well be detected by naked eye under ordinary light microscope when Pt cluster bind the αvβ3 in cell and catalyze none color 3,3'-Diaminobenzidine (DAB) into brown ones in situ. Moreover, Siha, Hela and 16HBE cell lines with different αvβ3 expression levels can be visually distinguished by the peroxidase-like Pt clusters. This research will provide a reliable method for the simple detection αvβ3 level in cells.