H₂PtCl₆ was purchased from the Shanghai Fine Chemical Materials Research Institute (AR, Shanghai, China). The peptide cyclo-(RGDfK)YCC was purchased from Beijing SciLight Biotechnology Co., Ltd. Phosphate buffer solution (PBS) and trypsin-EDTA were obtained from Gibco (NY, United States of America). Hydrogen peroxide (H₂O₂), sodium hydroxide (NaOH), nitric acid (HNO₃), and hydrochloric acid (HCl) were obtained from Beijing Chemical Reagent Co., Ltd. 3,3′,5,5′-Tetramethylbenzidine (TMB), DAB horseradish peroxidase color development kit, and YO-PRO-1/RNase staining solution for nucleus were obtained from Beyotime Biotech. Inc. Ultrapure water (18.2 MΩ) was used throughout the experiments.

Briefly, the H₂PtCl₆ (50 mM, 200 μL) solution was added to cyclo-(RGDfK)YCC (0.5 mM, 5 mL) solution under vigorous stirring at 42°C. Subsequently, the NaOH (1 M, 100 μL) solution was injected into the reaction system. After 12 h of reaction under dark conditions and gentle stirring, the cluster was purified and concentrated using an ultrafiltration tube (molecular weight cutoff: 3 kDa) to remove free peptides and ions.

The optical properties of Pt clusters were characterized using a spectrofluorometer (RF-5301, Shimadzu, Japan) and a UV-1800 spectrophotometer (Shimadzu, Japan). The Pt cluster sizes and zeta potential were recorded on a Zetasizer Nano (ZS90, UK) instrument. The molecular formula of Pt clusters was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS, ABI MALDI-TOF, United States) in the positive ion linear mode by using sinapinic acid as the matrix. The TEM image was obtained using an FEI Titan G2 (300 kV) transmission electron microscope.

After Pt clusters were bound to the integrin of the cell, free Pt clusters were washed out, and the integrin-bound Pt was measured using an inductively coupled plasma mass spectrometry (ICP-MS) analysis system (Thermo Elemental X7, United States). The cell was added to aqua regia (HCl: HNO₃ = 3:1) overnight, and then, the mixture was heated to 160°C. After the solution was concentrated to 10 mL, it was diluted with an aqueous solution containing 2% HNO₃ and 1% HCl to a final volume of 10 mL. All samples were injected into the ICP-MS system in turn, and each sample was measured three times to obtain an average value. Bismuth (20 ppb, containing 2% HNO₃ and 1% HCl) solution was used as the internal standard solution. By testing a series of Pt standard aqueous solutions (0.1, 0.5, 1, 5, 10, and 50 ng mL⁻¹), a standard plot of Pt standard concentration was obtained.

After Pt clusters catalyzed TMB in solution, the non-color TMB produced a soluble blue-colored TMB with absorbance at 652 nm. To verify the peroxidase-like activity of Pt clusters, Pt cluster solution (0, 20, 40, 80, 120, and 160 μM Pt) with 100 mM H₂O₂ and 800 µM TMB was incubated under neutral conditions for 5 min. Then, the color of the reaction solutions was monitored, and the absorbance at 652 nm was measured using a SpectraMax M2 microplate reader.

Human cervical carcinoma cell lines (SiHa and HeLa) and human bronchial epithelial cell lines (16HBE) were purchased from the Cancer Institute and Hospital, Chinese Academy of Medical Sciences. Cells were cultured in RPMI 1640 (HyClone, United States) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin (Gibco, United States). Three kinds of cells (2 × 10⁴ cells) were seeded and plated in a glass-bottom dish and incubated at 37°C for 24 h. After removing the medium, the cells were washed with PBS and fixed with 4% paraformaldehyde for 30 min. Then, 3% BSA was added and incubated for 1 h to block the non-specific recognition sites. The cells were then incubated with Pt clusters for 1 h. Untreated cells were used as control. Then, the nucleus was stained with YO-PRO-1/RNase Staining Solution (1 mL) for 10 min. Finally, the fluorescence images of cells were captured using a Nikon Ti-e microscope with a 60 × 1.4NA plan apochromatic oil immersion lens.

To further confirm the specificity of Pt clusters binding to α _v β ₃, we designed a competitive and blocking binding site study. In the competitive binding site study, the cells were incubated with PBS containing 5 mM RGD peptide and 120 μM Pt clusters for 1 h. The nucleus was then labeled with YO-PRO-1/RNase Staining Solution for 5 min. After washing with PBS three times, the cells were used for cell fluorescence imaging. In the blocking binding site study, the cells were incubated with 5 mM RGD (1 mL) solution for 1 h, washed with PBS, and then incubated with Pt cluster (120 μM, 1 mL) solution for another 1 h. The cells were then washed with PBS, and the nucleus was stained with YO-PRO-1/RNase Staining Solution. Finally, the cells were used for cell fluorescence imaging.

SiHa cells were fixed with 4% paraformaldehyde for 30 min, sealed with 3% BSA, washed with PBS, and incubated with Pt clusters of different Pt concentrations (0, 40, 80, 120, 160, and 200 μM) for 1 h. After imaging was completed, the DAB working solution was added and incubated at 37°C for 30 min. The cells were thoroughly washed with PBS, and cells dyed with brown DAB were observed using an ordinary optical microscope.

SiHa, HeLa, and 16HBE cells with different α _v β ₃ expressions were fixed with 4% paraformaldehyde, and the non-specific binding sites of fixed cells, with 3% BSA. The cells were incubated with 120 μM Pt clusters for 1 h and then washed with PBS, and fluorescence imaging was performed with the CLSM system. For all three cell lines, the DAB working solution was added and incubated at 37°C for 30 min. After cleaning with PBS, they were observed under an ordinary light microscope.

The non-specific binding sites of fixed cells were first blocked with 3% BSA. These fixed cells were incubated with 120 μM Pt clusters for 1 h to specifically label. The sample was transferred to a beaker and pre-digested with 3 mL nitric acid and 1 mL hydrogen peroxide for 12 h. After being concentrated to 0.1 mL at 160°C, the mixture was digested with aqua regia for another 12 h and then was concentrated again to 0.1 mL. Finally, an aqueous solution containing 2% HNO₃ and 1% HCl was used to dilute the concentrated solution to an appropriate volume. The Pt content in the diluted solution was determined by ICP-MS.

Fluorescence intensity analysis: in brief, a blue fluorescence image was opened, converted to 8-bit, inverted, uncalibrated OD, scale was set, measurements and threshold were set, and measured. DAB intensity analysis: by measuring the gray value, DAB intensity was reflected, a DAB image was opened, converted to 8-bit, uncalibrated OD, measurement and threshold were set, and measured. All data are represented as the mean ± SD. Statistical comparisons were conducted using one-way analysis of variance (ANOVA). *p < 0.1, **p < 0.05, and ***p < 0.001 were defined as statistically significant difference.

Figure 2A illustrates the synthesis of Pt clusters, the cyclo-(RGDfK)YCC peptide that contains two functional domains, domain cyclo-(RGDfK) is a specific sequence of targeted αvβ3 (Nieberler et al., 2017), and CCY can reduce Pt⁴⁺ to Pt atoms and bind Pt atoms via sulfhydryl groups of cysteine (Zhang et al., 2020). The peptide-coated Pt clusters were further purified and used for a follow-up study. The fluorescence characteristics of the Pt clusters were measured by fluorescence spectrophotometry. The maximum excitation peak of the Pt cluster was 306 nm (Figure 2B, black curve), and the maximum emission peak was 418 nm (Figure 2B, blue curve). The Pt cluster fluorescence was highly stable at 4°C in the system of H₂O with no significant changes for over 18 days (Supplementary Figure S1). The transparent Pt cluster solution appears pale yellow under natural light and fluorescently blue under UV light (Figure 2B). In addition, the formation of Pt clusters was determined by ultraviolet visible spectrophotometry. Pt⁴⁺ in aqueous solution had a strong absorption at 260 nm (Figure 2C, black line), and the absorption peak of the peptide was at 275 nm (Figure 2C, red line), which was attributed to the π–π conjugate structure of the phenol side group in tyrosine (Zhang et al., 2021; Zhang et al., 2022). There was no obvious absorption peak after the formation of Pt clusters. Dynamic light scattering (DLS) measurements show that the Pt cluster has a hydrodynamic diameter of about 2.01 nm (Figure 2D). A typical transmission electron microscopic (TEM) image is presented in Supplementary Figure S2. Furthermore, the precise composition of the Pt cluster was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) (Luo et al., 2012; Du et al., 2022). Figure 2E illustrates a series of strong peaks between 2,500 and 10,000 m/z in the mass spectrum. The mass spectra are composed of serial main peaks with a space of about 1168 m/z between adjacent peaks, which matches the molecular weight of one Pt atom and a peptide. The strongest main peak was located at around 7,207 m/z, which matches the peak of Pt₁₇ (RGD)₄.

We first evaluated the enzyme-like catalytic activity of Pt clusters by employing TMB and H₂O₂ as the substrates. In the presence of H₂O₂ and Pt clusters, the amino group of colorless TMB loses an electron to become a cationic radical and exists in the system in the form of a dimer charge-transfer complex. The dimer has the maximum absorption at 652 nm and appears blue. In an acidic environment, the complex loses another electron and is further oxidized into a yellow quinone conjugate monomer structure (Figure 3A). After the mixture of TMB and hydrogen peroxide was injected into Pt clusters of different concentrations, the colorless solution gradually turned blue. With the increase of Pt cluster concentration, the intensity of blue gradually increased (Figure 3B), and the maximum absorbance at 652 nm also gradually increased (Figure 3C). Without the introduction of Pt clusters, the color of TMB and the maximum absorbance did not change.

Due to the targeting of RGD peptides, peptide-coated Pt clusters can bind with αvβ3 expression in cells (Guo et al., 2014; Yuan et al., 2014; Ludwig et al., 2021; Pretze et al., 2021; Pang et al., 2023; Yin et al., 2023). Confocal laser scanning microscopy (CLSM) was used to verify the specificity target of Pt in SiHa cells. As expected, there was obvious blue fluorescence, as shown in Figure 4A, but no fluorescence in the control group (Figure 4D). This suggests Pt clusters are located on the cell membrane of SiHa cells. In addition, peptide competition and blocking experiments were performed to confirm the specific targeting of Pt clusters to αvβ3. When Pt clusters and free RGD peptides were added to cells, there was almost no blue fluorescence in the cells (Figure 4B), indicating that Pt clusters competed with free RGD peptides for the same binding site. When Pt clusters were introduced into cells after previously blocked by a 5 mM free RGD peptide, no blue fluorescence was observed in the cells (Figure 4C), suggesting that the integrin-binding site had been occupied by the free RGD peptide. The corresponding fluorescence intensity was analyzed by ImageJ software (Supplementary Figure S3). These results suggest that Pt clusters can specifically target αvβ3 on SiHa cells.

To detect αvβ3 in the cell membrane more accurately, the optimal concentration of Pt cluster incubation with SiHa cells was studied. The fluorescence intensity of SiHa cells increased as the Pt cluster concentration increased from 0 to 120 μM (Figure 5A). The fluorescence intensity of SiHa cells stopped changing when the Pt cluster concentration reached 120 μM. In cell-cultured medium, when the Pt cluster concentration continued to increase over 120 μM, the fluorescence intensity did not increase significantly. This is consistent with the fluorescence intensity analyzed by ImageJ software (Figure 5B). After the cells were detected using the CLSM system, the DAB working solution was introduced into the cells previously incubated by the Pt cluster. Under the Pt cluster catalytic oxidation, DAB produced a brown sediment in the cells. The brown intensity of DAB increased as the Pt cluster concentration increased from 0 to 120 μM (Figure 5C). When the Pt cluster concentration reached 120 μM, the brown intensity of SiHa cells did not increase. The brown intensity on the cells was analyzed using ImageJ software. The result is consistent with naked eye observation under a normal light microscope. These results indicate that the saturation concentration of labeled αvβ3 can be achieved when the concentration of Pt clusters is 120 μM in the cell medium.

To verify whether Pt clusters can easily and rapidly detect integrin expression on different cell lines, we selected SiHa, HeLa, and 16HBE cell lines with different integrin expressions as models. These cells were incubated with Pt clusters and observed using a fluorescence microscope. After this, the DAB working solution was added to the Pt cluster-labeled cells and observed using an optical microscope. As shown in Figures 6A,B, the brown sediment in SiHa cells was deeper than that in HeLa cells, indicating that SiHa cells expressed more integrins than HeLa cells. There was weak brown color on the surface of the 16HBE cell membrane, indicating that 16HBE cells expressed integrin αvβ3 proteins at a low level. Brown intensity statistics also showed that SiHa cells had the highest expression of integrin αvβ3 among the three cell lines (Figure 6C). The results were confirmed by changes in Pt cluster fluorescence intensity in the CLSM system (Figures 6A,B). The intensity of blue fluorescence in SiHa cells was stronger than that in HeLa cells, while it was almost undetectable in 16HBE cells. In addition, Pt clusters in SiHa and HeLa cells were quantified by ICP-MS, and the Pt mass in SiHa and HeLa cells was 83.16 fg/cell and 25.78 fg/cell, respectively. The near-absence of Pt in 16HBE cells further demonstrates that Pt clusters can differentiate integrin protein expression between different cell lines.