AUTHOR=Chang Stephen S. , Huang Hung-Tse , Wei Wen-Chi , Lo I-Wen , Lin Yu-Chi , Chao Chih-Hua , Liao Geng-You , Shen Yuh-Chiang , Chen Jih-Jung , Li Tsung-Lin , Lin Liang-Tzung , Tai Chen-Jei , Kuo Yao-Haur , Liaw Chia-Ching 

TITLE=Anti-inflammatory effect of euphane- and tirucallane-type triterpenes isolated from the traditional herb Euphorbia neriifolia L

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1223335

DOI=10.3389/fchem.2023.1223335

ISSN=2296-2646

ABSTRACT=The Euphorbiaceae plant Euphorbia neriifolia L. is distributed widely in India, Thailand, Southeastern China, and Taiwan and has been used to treat such inflammation-related diseases such as carminative, expectorant, gonorrhoea, asthma, and cancer. In the course of our search for potential antiinflammatory agents from the title plant, eleven triterpenes from the stem of E. neriifolia were isolated Anti-inflammatory compounds from E. neriifolia 2 and reported in our previous endeavor. Given its rich abundance in triterpenoids, the ethanolic extract in this follw-up exploration has led to isolation of additional eight triterpenes, including six new euphanes, neritriterpenols H and J-N (1 and 3-7), one new tirucallane, neritriterpenol I (2), and a known compound, 11-oxo-kansenonol (8). Their chemical structures were elucidated on the basis of spectroscopic data, including 1D-and 2D NMR, and HRESIMS spectra. The absolute stereochemistry of neritriterpenols was determined by single crystal X-ray diffraction analysis, ICD spectra, and DP4+ NMR data calculations. Compounds 1-8 were also evaluated for their anti-inflammatory activity by using lipopolysaccharide (LPS)-stimulated IL-6 and TNF-α on RAW264.7 macrophage cells. Intriguingly, the euphane-type triterpenes (1 and 3-8) showed an inhibitory effect on LPS-induced IL-6 but not on TNF-α, while tirucallane-type triterpene 2 showed strong inhibition on both IL-6 and TNFα.