AUTHOR=Shaheen Asma , Akram Sumia , Sharif Saima , Rashid Ayoub , Adnan Ahmad , Mushtaq Muhammad 

TITLE=Fractionation of Xanthium strumarium L. foliage phenolics, in-vitro antioxidant activities, and in-vivo anti-diabetic potential

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1279729

DOI=10.3389/fchem.2023.1279729

ISSN=2296-2646

ABSTRACT=The present research aimed to fractionate Xanthium strumarium L. (XSL) foliage phenolics into a set of solvents and evaluate their antioxidant potential and in-vivo anti-diabetic activity against Alloxan monohydrate-induced diabetic mice. For this purpose, XSL foliage was fractionated into petroleum ether, ethyl acetate, ethanol, and water via orbital type shaking and tested for the presence of phenolics, their antioxidant and  antidiabetic potential.. The results revealed that the ethyl acetate fraction of XSL foliage contained the highest amount of total phenolics 95.25 mg GAE/g of extract, followed by ethanol (65.14 mg GAE/g), petroleum ether (25.12 mg GAE/g), water (12.20 mg GAE/g), and XSL powder (69.13 mg GAE/g). At the end of treatment time (18th day of oral administration of 400 mg/kg body weight of mice),  the  ethyl acetate fraction significantly (p≤0.05) lowered blood glucose level ( 353±10.6 to 220±25.5 mg/dl), . . Overall, it can be speculated that ethyl acetate and ethanol may work efficiently for the enrichment of XSL phenolic without compromising their antidiabetic potential.