AUTHOR=Tippannanavar Madhu , Banerjee Tirthankar , Shekhar Sumit , Sahu Sudama Ram , Singh Bijendra , Narayanan Neethu , Rudra Shalini Gaur , Chakrabarti Bidisha , Gupta Suman , Singh Anupama 

TITLE=Development, validation and a GAPI greenness assessment for the determination of 103 pesticides in mango fruit drink using LC-MS/MS

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 11 - 2023

YEAR=2023

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2023.1283895

DOI=10.3389/fchem.2023.1283895

ISSN=2296-2646

ABSTRACT=A robust method was developed using LC-ESI-MS/MS based identification and quantification of 103 fortified pesticides in mango fruit drink. Variations in QuEChERS extraction (without buffer, citrate and acetate buffered) coupled with dispersive clean-up combinations were evaluated. Results showed, 5 mL dilution and citrate buffered QuEChERS extraction with anhy. MgSO4 clean-up gave acceptable recovery for 100 pesticides @ 1 µg mL-1 fortification. The method was validated as per SANTE guidelines (SANTE/11813/2021). 95, 91 and 77 pesticides could bewere satisfactorily recovered at @ 0.1, 0.05, and 0.01 µg mL-1 fortification with HorRat values ranging formed 0.2 - 0.8 for majority. The method showed mild matrix enhancement effect for 66%77 pesticides with global uncertainty of 4.72 to 239.8926%. The rReliability of the method was confirmed by real sample analysis of different brands of mango drinks available in market. The greenness assessment by GAPI (Green Analytical Procedure Index) indicated the method was much greener than other contemporary methods.