AUTHOR=Chen Ziwei , Wang Jiajia , Zhou Jianyu , Yu Haifeng , Zheng Lu , Chen Yuan , Wan Xiaoqing , Zhang Wei 

TITLE=Tripterygium drug-loaded liposome alleviates renal function by promoting vascularization and inhibiting fibrosis

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 12 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2024.1427670

DOI=10.3389/fchem.2024.1427670

ISSN=2296-2646

ABSTRACT=Objective: To construct a drug-modified renal infarction targeting liposome (rTor-LIP) containing the tripterygium in order to improve the therapeutic effect of renal injury. Methods: rTor-LIP was prepared by extruder method containing tripterygium solution; The preparation was characterized by transmission electron microscopy, marvin laser particle size analyzer and western blotting; In vitro experiments verify the biocompatibility of rTor-LIP, and in vivo experiments verify the therapeutic effect of rTor-LIP on renal injury. Results: The surface of rTor-LIP was regular and oval. In vitro results showed that after co incubation with rTor-LIP, endothelial cells did not show significant apoptosis, and there were no significant abnormalities in mitochondrial metabolism (P<0.05). The results in vivo showed that the morphology of endothelial cells in rTor-LIP group was uniform, the cytoplasmic striations were clear, but the local striations disappeared. Conclusion: rTor-LIP nano-targeted liposomes can effectively target hypoxic kidney tissue, providing a new idea for the treatment of renal infarction.