AUTHOR=Murata Takatsugu , Komukai Kyoka , Semba Yuta , Murata Eri , Sato Fumi , Takano Tomohiro , Tsuchiya Kaho , Matsuda Chihiro , Sakai Anju , Yoneoka Amane , Takahashi Shunsuke , Nagahara Yukitoshi , Shiina Isamu 

TITLE=Synthesis of BODIPY FL-tethered ridaifen-B, RID-B-BODIPY, and its localization in cancer cells

JOURNAL=Frontiers in Chemistry

VOLUME=Volume 12 - 2024

YEAR=2024

URL=https://www.frontiersin.org/journals/chemistry/articles/10.3389/fchem.2024.1451468

DOI=10.3389/fchem.2024.1451468

ISSN=2296-2646

ABSTRACT=We synthesized Ridaifen-B Boron dipyrromethene (RID-B-BODIPY) using 2-methyl-6-nitro benzoic anhydride (MNBA)-mediated dehydration condensation reaction between amino alkyltethered RID and BODIPY FL. Comparative experiments between dicyclohexylcarbodiimide (DCC) and MNBA for their coupling reactions demonstrated that MNBA is an effective condensation reagent for amines and BODIPY FL. A cell staining study with RID-B-BODIPY showed intracellular localization of BODIPY FL fluorescence, attributed to the RID-B structure, indicating the successful development of a tool for analyzing intracellular molecular behavior efficiently.