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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Earth Sci.</journal-id>
<journal-title>Frontiers in Earth Science</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Earth Sci.</abbrev-journal-title>
<issn pub-type="epub">2296-6463</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">785396</article-id>
<article-id pub-id-type="doi">10.3389/feart.2021.785396</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Earth Science</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Acoustic Predictors of Active Fluid Expulsion From a Hydrothermal Vent Field, Offshore Taup&#x14d; Volcanic Zone, New&#x20;Zealand</article-title>
<alt-title alt-title-type="left-running-head">Spain et&#x20;al.</alt-title>
<alt-title alt-title-type="right-running-head">Acoustic Predictors Seafloor Fluid Expulsion</alt-title>
</title-group>
<contrib-group>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Spain</surname>
<given-names>Erica</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
<uri xlink:href="https://loop.frontiersin.org/people/819571/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Lamarche</surname>
<given-names>Geoffroy</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/637350/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Lucieer</surname>
<given-names>Vanessa</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/764662/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Watson</surname>
<given-names>Sally J.</given-names>
</name>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/848044/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Ladroit</surname>
<given-names>Yoann</given-names>
</name>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff4">
<sup>4</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/772406/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Heffron</surname>
<given-names>Erin</given-names>
</name>
<xref ref-type="aff" rid="aff5">
<sup>5</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1630491/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Pallentin</surname>
<given-names>Arne</given-names>
</name>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1625887/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Whittaker</surname>
<given-names>Joanne M.</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/243237/overview"/>
</contrib>
</contrib-group>
<aff id="aff1">
<sup>1</sup>
<institution>Institute for Marine and Antarctic Studies</institution>, <institution>University of Tasmania</institution>, <addr-line>Hobart</addr-line>, <addr-line>TAS</addr-line>, <country>Australia</country>
</aff>
<aff id="aff2">
<sup>2</sup>
<institution>National Institute of Water and Atmospheric Research</institution>, <addr-line>Wellington</addr-line>, <country>New&#x20;Zealand</country>
</aff>
<aff id="aff3">
<sup>3</sup>
<institution>School of Environment</institution>, <institution>University of Auckland</institution>, <addr-line>Auckland</addr-line>, <country>New&#x20;Zealand</country>
</aff>
<aff id="aff4">
<sup>4</sup>
<institution>ENSTA Bretagne</institution>, <addr-line>Brest</addr-line>, <country>France</country>
</aff>
<aff id="aff5">
<sup>5</sup>
<institution>Center for Coastal and Ocean Mapping</institution>, <institution>Joint Hydrographic Center</institution>, <institution>University of New Hampshire</institution>, <addr-line>Durham</addr-line>, <addr-line>NH</addr-line>, <country>United&#x20;States</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>
<bold>Edited by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/476389/overview">Alessandra Savini</ext-link>, University of Milano-Bicocca, Italy</p>
</fn>
<fn fn-type="edited-by">
<p>
<bold>Reviewed by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1254503/overview">Adam Skarke</ext-link>, Mississippi State University, United&#x20;States</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/612765/overview">Craig John Brown</ext-link>, Dalhousie University, Canada</p>
</fn>
<corresp id="c001">&#x2a;Correspondence: Erica Spain, <email>erica.spain@niwa.co.nz</email>
</corresp>
<fn fn-type="other">
<p>This article was submitted to Marine Geoscience, a section of the journal Frontiers in Earth Science</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>24</day>
<month>01</month>
<year>2022</year>
</pub-date>
<pub-date pub-type="collection">
<year>2021</year>
</pub-date>
<volume>9</volume>
<elocation-id>785396</elocation-id>
<history>
<date date-type="received">
<day>29</day>
<month>09</month>
<year>2021</year>
</date>
<date date-type="accepted">
<day>22</day>
<month>12</month>
<year>2021</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2022 Spain, Lamarche, Lucieer, Watson, Ladroit, Heffron, Pallentin and Whittaker.</copyright-statement>
<copyright-year>2022</copyright-year>
<copyright-holder>Spain, Lamarche, Lucieer, Watson, Ladroit, Heffron, Pallentin and Whittaker</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these&#x20;terms.</p>
</license>
</permissions>
<abstract>
<p>Understanding fluid expulsion is key to estimating gas exchange volumes between the seafloor, ocean, and atmosphere; for locating key ecosystems; and geohazard modelling. Locating active seafloor fluid expulsion typically requires acoustic backscatter data. Areas of very-high seafloor backscatter, or &#x201c;hardgrounds,&#x201d; are often used as first-pass indicators of potential fluid expulsion. However, varying and inconsistent spatial relationships between active fluid expulsion and hardgrounds means a direct link remains unclear. Here, we investigate the links between water-column acoustic flares to seafloor backscatter and bathymetric metrics generated from two calibrated multibeam echosounders. Our site, the Calypso hydrothermal vent field (HVF) in the Bay of Plenty, Aotearoa/New&#x20;Zealand, has an extensive catalogue of vents and seeps in &#x3c;250&#xa0;m water depth. We demonstrate a method to quantitatively link active fluid expulsion (flares) with seafloor characteristics. This allows us to develop predictive spatial models of active fluid expulsion. We explore whether data from a low (30&#xa0;kHz), high (200&#xa0;kHz), or combined frequency model increases predictive accuracy of expulsion locations. This research investigates the role of hardgrounds or surrounding sediment cover on the accuracy of predictive models. Our models link active fluid expulsion to specific seafloor characteristics. A combined model using both the 30 and 200&#xa0;kHz mosaics produced the best results (predictive accuracy: 0.75; Kappa: 0.65). This model performed better than the same model using individual frequency mosaics as input. Model results reveal active fluid expulsion is not typically associated with the extensive, embedded hardgrounds of the Calypso HVF, with minimal fluid expulsion. Unconsolidated sediment around the perimeter of and between hardgrounds were more active fluid expulsion sites. Fluids exploit permeable pathways up to the seafloor, modifying and refashioning the seafloor. Once a conduit self-seals, fluid will migrate to a more permeable pathway, thus reducing a one-to-one link between activity and hardgrounds. Being able to remotely predict active and inactive regions of fluid expulsion will prove a useful tool in rapidly identifying seeps in legacy datasets, as well as textural metrics that will aid in locating nascent, senescent, or extinct seeps when a survey is underway.</p>
</abstract>
<kwd-group>
<kwd>hydrothermal vent areas</kwd>
<kwd>New Zealand</kwd>
<kwd>seeps</kwd>
<kwd>random forest</kwd>
<kwd>seafloor</kwd>
<kwd>calypso vents</kwd>
<kwd>acoustic seafloor backscatter</kwd>
</kwd-group>
<contract-sponsor id="cn001">Australian Research Council<named-content content-type="fundref-id">10.13039/501100000923</named-content>
</contract-sponsor>
<contract-sponsor id="cn002">Royal Society Te Ap&#x101;rangi<named-content content-type="fundref-id">10.13039/501100001509</named-content>
</contract-sponsor>
<contract-sponsor id="cn003">Ministry for Business Innovation and Employment<named-content content-type="fundref-id">10.13039/501100004629</named-content>
</contract-sponsor>
</article-meta>
</front>
<body>
<sec id="s1">
<title>Introduction</title>
<p>Seafloor fluid expulsion is a key mechanism for large-scale gas exchange between the seafloor, ocean (<xref ref-type="bibr" rid="B53">Mcginnis et&#x20;al., 2006</xref>; <xref ref-type="bibr" rid="B68">Reeburgh, 2007</xref>), and potentially the atmosphere (<xref ref-type="bibr" rid="B22">Gentz et&#x20;al., 2014</xref>; <xref ref-type="bibr" rid="B70">Sahling et&#x20;al., 2014</xref>; <xref ref-type="bibr" rid="B74">Shakhova et&#x20;al., 2014</xref>; <xref ref-type="bibr" rid="B69">R&#xf6;mer et&#x20;al., 2017</xref>). Anomalous areas of very-high seafloor acoustic backscatter, or &#x201c;hardgrounds,&#x201d; are often used as first-pass indicators of fluid expulsion sites, in addition to water-column acoustic anomalies, or &#x201c;flares.&#x201d; Hardgrounds form via several seafloor fluid expulsion processes: persistent mineral precipitation of oxides, sulfides, and minerals from hydrothermal fluids (<xref ref-type="bibr" rid="B75">Stoffers et&#x20;al., 1999</xref>; <xref ref-type="bibr" rid="B12">Canet, 2003</xref>; <xref ref-type="bibr" rid="B67">Prol-Ledesma et&#x20;al., 2004</xref>; <xref ref-type="bibr" rid="B63">No&#xe9; et&#x20;al., 2006</xref>); hydrothermal alteration of the seafloor (<xref ref-type="bibr" rid="B28">Hocking et&#x20;al., 2010</xref>; <xref ref-type="bibr" rid="B59">Monecke et&#x20;al., 2014</xref>); or microbial mediation, whereby archaeal microbes oxidize dissolved gasses in escaping bubbles, forming authigenic carbonate platforms (<xref ref-type="bibr" rid="B43">Levin, 2005</xref>; <xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>; <xref ref-type="bibr" rid="B18">Finkl and Makowski, 2016</xref>). Anomalous very-high seafloor backscatter areas have higher backscatter intensities than the surrounding seafloor are thus interpreted as &#x2018;hardgrounds,&#x2019; as lithified seafloor is a stronger scatterer than the surrounding soft sediment. The relationship between acoustic hardgrounds and observable areas of indurated, lithified seafloor indicative of these processes has been validated in numerous settings (<xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>; <xref ref-type="bibr" rid="B13">Conti et&#x20;al., 2016</xref>; <xref ref-type="bibr" rid="B56">Mitchell et&#x20;al., 2018</xref>; <xref ref-type="bibr" rid="B76">Thorsnes et&#x20;al., 2019</xref>; <xref ref-type="bibr" rid="B65">Phrampus et&#x20;al., 2020</xref>; <xref ref-type="bibr" rid="B79">Watson et&#x20;al., 2020</xref>).</p>
<p>A direct link between active fluid expulsion and acoustic hardgrounds however remains unclear. Varying and inconsistent spatial relationships between observable fluid expulsion, hardgrounds, and the surrounding seafloor have been noted previously (<xref ref-type="bibr" rid="B61">Naudts et&#x20;al., 2008</xref>; <xref ref-type="bibr" rid="B62">Naudts et&#x20;al., 2010</xref>; <xref ref-type="bibr" rid="B76">Thorsnes et&#x20;al., 2019</xref>). This unclear relationship reflects the complexity of hardground genesis, gas migration, fluid expulsion processes, and spatio-temporal differences in expulsion and embedment. Active fluid expulsion can be both short- (decades) or long-lived (thousands of years) (<xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>; <xref ref-type="bibr" rid="B42">Levin et&#x20;al., 2016</xref>). Fluids exploit permeable pathways upward to the seafloor, with preliminary seafloor expression (either liquid or gas bubble expulsion) often occurring in areas of soft unconsolidated sediment due to an unrestricted pathway (<xref ref-type="bibr" rid="B43">Levin, 2005</xref>; <xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>). Hardground formation begins within the subsurface and, given enough time, can become embedded on the seafloor. Once the conduit closes or ceases activity, hardgrounds will remain at the surface or in the subsurface, with surface hardgrounds thenceforth observable in seafloor acoustic backscatter (<xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>; <xref ref-type="bibr" rid="B42">Levin et&#x20;al., 2016</xref>). Formalizing the link between gas flares and hardgrounds has thus remained elusive due to spatiotemporal differences between active expulsion and hardground formation, which can post-date expulsion activity.</p>
<p>Here, we present a study from the active Calypso hydrothermal vent field (HVF), in the offshore Taup&#x14d; Volcanic Zone, Aotearoa/New&#x20;Zealand to address whether there are measurable links between gas flares, as a proxy for active fluid expulsion, and seafloor characteristics, in particular hardgrounds. Calypso HVF represents a rich laboratory of active fluid expulsion, in gas and liquid form, with a mix of vents, seeps, and hardground features, with a formal catalogue of active fluid expulsion spanning over five decades (<xref ref-type="bibr" rid="B16">Duncan and Pantin, 1969</xref>; <xref ref-type="bibr" rid="B23">Glasby, 1971</xref>; <xref ref-type="bibr" rid="B71">Sarano et&#x20;al., 1989</xref>; <xref ref-type="bibr" rid="B75">Stoffers et&#x20;al., 1999</xref>; <xref ref-type="bibr" rid="B6">Botz et&#x20;al., 2002</xref>; <xref ref-type="bibr" rid="B57">Mitchell et&#x20;al., 2004</xref>; <xref ref-type="bibr" rid="B39">Lamarche and Barnes, 2005</xref>; <xref ref-type="bibr" rid="B73">Schwarz-Schampera et&#x20;al., 2007</xref>; <xref ref-type="bibr" rid="B28">Hocking et&#x20;al., 2010</xref>; <xref ref-type="bibr" rid="B82">Wysoczanski et&#x20;al., 2015</xref>).</p>
<p>Our dataset encompasses bathymetry and backscatter from two calibrated multibeam echosounders (MBES) operating simultaneously at 200 and 30&#xa0;kHz (nominal) frequencies. Seafloor backscatter reflectivity and penetration depends, in part, on the signal frequency or aperture of the chosen MBES, as well as the acoustic impedance characteristics of the seafloor (a function of the medium&#x2019;s density and consequent sound speed) (<xref ref-type="bibr" rid="B31">Jackson et&#x20;al., 1986</xref>; <xref ref-type="bibr" rid="B24">Guillon and Lurton, 2001</xref>; <xref ref-type="bibr" rid="B29">Huff, 2008</xref>). The acoustic response, or backscatter intensity returned, is dependent on interactions between wavelength, seafloor roughness, seafloor scattering regime (a function of substrate particle size), and volume scattering effects (a function of signal penetration), once radiometric and geometric compensations are applied (<xref ref-type="bibr" rid="B77">Urick, 1956</xref>; <xref ref-type="bibr" rid="B54">Medwin and Clay, 1997</xref>; <xref ref-type="bibr" rid="B30">Hughes Clarke, 2015</xref>). The seafloor backscatter mosaic produced may therefore not directly correlate to the feature of interest if the MBES penetration potential is too high or low. Specific to hardgrounds, the MBES frequency may be unable to reveal hardgrounds embedded beneath the surface unless it can penetrate to the appropriate depths or may not accurately capture surface expressions if the frequency is too&#x20;low.</p>
<p>Here, we use a classification approach, involving image segmentation and random forest modelling, to:<list list-type="simple">
<list-item>
<p>1 Quantitatively link water-column acoustic flares (here considered as a proxy for active fluid expulsion) with specific seafloor characteristics to determine potential links to hardgrounds in this region;</p>
</list-item>
<list-item>
<p>2 Assess whether these specific seafloor characteristics can be used to develop predictive spatial models of active fluid expulsion;</p>
</list-item>
<list-item>
<p>3 Evaluate if a low (nominally 30&#xa0;kHz), high (200&#xa0;kHz), or a combined frequency model increases predictive accuracy of seafloor fluid expulsion;&#x20;and</p>
</list-item>
<list-item>
<p>4 Investigate the role hardgrounds or surrounding sediment cover may have on the accuracy of a predictive&#x20;model.</p>
</list-item>
</list>
</p>
</sec>
<sec sec-type="materials|methods" id="s2">
<title>Materials and Methods</title>
<sec id="s2-1">
<title>The Calypso Hydrothermal Vent Field</title>
<p>The Calypso HVF is a well-known gas-hydrothermal region of the offshore Taup&#x14d; Volcanic Zone (TVZ), in the center of the Bay of Plenty, Aotearoa/New&#x20;Zealand. It lies &#x223c;40&#xa0;km offshore Te Ika-a-M&#x101;ui/North Island and 7&#xa0;km SW of the Whakaari/White Island active volcano (<xref ref-type="fig" rid="F1">Figure&#x20;1A</xref>) (<xref ref-type="bibr" rid="B71">Sarano et&#x20;al., 1989</xref>; <xref ref-type="bibr" rid="B75">Stoffers et&#x20;al., 1999</xref>; <xref ref-type="bibr" rid="B6">Botz et&#x20;al., 2002</xref>). The Calypso HVF is part of the Central Volcanic Region, that represents the volcanically active back-arc region of the Hikurangi subduction system located ca. 300&#xa0;km to the east. The extensional tectonic setting associated with the back-arc has resulted in a dense network of NE-SW trending normal faults (<xref ref-type="bibr" rid="B81">Wright, 1992</xref>), expressed at the seafloor by a pervasive system of scarps (<xref ref-type="bibr" rid="B39">Lamarche and Barnes, 2005</xref>). The TVZ extends offshore from the Bay of Plenty coast into the NE-trending depression of the Whakat&#x101;ne Graben.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption>
<p>
<bold>(A)</bold> Bay of Plenty, Aotearoa/New&#x20;Zealand (inset map) bathymetry, the offshore Taup&#x14d; Volcanic Zone (TVZ). The Calypso HVF (black box) sits between Whakaari/White Island and Moutohor&#x101;/Whale Island (black dashed lines: TVZ approximate boundaries; white lines: 100&#xa0;m contours;). <bold>(B)</bold> Calypso HVF 5&#xa0;m bathymetry, collected during TAN1806, draped over regional bathymetry (white lines: 50&#xa0;m contours; pink lines: vessel track; white dashed boxes: clipped North and South Calypso HVF regions; white boxes: towed camera locations; black crosses: sediment grab locations).</p>
</caption>
<graphic xlink:href="feart-09-785396-g001.tif"/>
</fig>
<p>Active fluid expulsion was formally catalogued at Calypso HVF from water-column acoustic scattering and visible sea surface bubbles (<xref ref-type="bibr" rid="B16">Duncan and Pantin, 1969</xref>; <xref ref-type="bibr" rid="B23">Glasby, 1971</xref>) and then from video observations (<xref ref-type="bibr" rid="B71">Sarano et&#x20;al., 1989</xref>; <xref ref-type="bibr" rid="B28">Hocking et&#x20;al., 2010</xref>). These fluid expressions are evidence of both diffuse and discrete gas emission, predominantly CH<sub>4</sub> and CO<sub>2</sub>, from submarine geothermal activity (<xref ref-type="bibr" rid="B75">Stoffers et&#x20;al., 1999</xref>; <xref ref-type="bibr" rid="B6">Botz et&#x20;al., 2002</xref>). Calypso HVF fluid expulsion is suggested to be structurally controlled by the pervasive NE-SW trending fault system, with observed fluid expulsion ephemerality inferred to be related to seismic activity (<xref ref-type="bibr" rid="B64">Pantin and Wright, 1994</xref>). <xref ref-type="bibr" rid="B75">Stoffers et&#x20;al. (1999)</xref> observed numerous active fluid expulsion areas aligned with fault scarps. In addition to the North Calypso HVF (including the original Calypso site from <xref ref-type="bibr" rid="B71">Sarano et&#x20;al. (1989)</xref>) a second site, now known as the South Calypso HVF, was also detected with two discrete zones of fluid expulsion (<xref ref-type="bibr" rid="B75">Stoffers et&#x20;al., 1999</xref>). Subsequent voyages produced bathymetric maps of the area (25&#xa0;m grid resolution) using a Kongsberg EM302 (30&#xa0;kHz) MBES and generated a good knowledge of the tectonic fabric along with surface, water-column, and seafloor evidence for fluid expulsion and mineral precipitation (<xref ref-type="bibr" rid="B57">Mitchell et&#x20;al., 2004</xref>; <xref ref-type="bibr" rid="B73">Schwarz-Schampera et&#x20;al., 2007</xref>; <xref ref-type="bibr" rid="B82">Wysoczanski et&#x20;al., 2015</xref>).</p>
</sec>
<sec id="s2-2">
<title>Multibeam Data Acquisition and Processing</title>
<p>Data were acquired during the 2018 RV <italic>Tangaroa</italic> voyage TAN 1806, a component of the international project QUOI: &#x201c;Quantitative Ocean-column Imaging using hydroacoustic sources&#x201d; (<xref ref-type="bibr" rid="B40">Lamarche et&#x20;al., 2019</xref>). The TAN1806 survey employed Kongsberg EM 2040 (200&#xa0;kHz) and EM302 (30&#xa0;kHz) MBES to map the Calypso HVF, operated synchronously. Both MBES systems are hull-mounted on RV <italic>Tangaroa</italic>. MBES compensation occurred at the start of the voyage at the NIWA test-patch sites in Palliser Bay and Palliser Bank, Aotearoa/New&#x20;Zealand. Calibration was undertaken over multiple depths on seafloor zones with flat, homogenous sedimentology of silt or sand (<xref ref-type="bibr" rid="B41">Lamarche et&#x20;al., 2011</xref>; <xref ref-type="bibr" rid="B49">Lucieer and Lamarche, 2011</xref>; <xref ref-type="bibr" rid="B38">Ladroit et&#x20;al., 2017</xref>). Lines were run for each MBES setting (frequency/pulse) anticipated. Backscatter correction curves were generated to compensate for beam pattern variations by: 1) removing all Kongsberg backscatter corrections, 2) modeling the backscatter angular variations, and 3) modeling the EM302 transducer beam pattern.</p>
<p>Sound velocity profiles (SVP) were taken at 12-h intervals during operations, 3-h intervals during compensation, at the start of new mapping areas, or when noticeable artefacts appeared in the data. MBES data were acquired via Kongsberg Seafloor Information System (SIS) software (v4.2.1) and cleaned in Caris HIPS/SIPS (v10.4). Vessel position, motion, SVP, and tidal corrections were merged with raw range/angle data to generate geolocated soundings. Manual cleaning removed remaining invalid soundings and data spikes. A bathymetric grid at 5&#xa0;m resolution was produced in NZTM projection (EPSG:2193) (<xref ref-type="fig" rid="F1">Figure&#x20;1B</xref>).</p>
</sec>
<sec id="s2-3">
<title>Active Fluid Expulsion Identification</title>
<p>Active fluid expulsion was identified in the EM302 MBES (30&#xa0;kHz) water-column backscatter data using the QPS FM Mid-Water (v7.7.4) Feature Detect tool. Acoustic anomalies detected were analyzed in QPS Fledermaus (v7.7.4) (<xref ref-type="fig" rid="F2">Figure&#x20;2</xref>). Acoustic anomalies that were attached to the seafloor, &#x201c;flares,&#x201d; were classed as potential fluid expulsion features or &#x201c;seeps,&#x201d; indicative of gas bubbles emitting from the seafloor. Point clusters of potential seeps were exported to MATLAB and filtered based on a maximum distance between points (40&#xa0;m), minimum number of points (200), and aspect ratio (1) of each cluster. A seafloor or &#x201c;seep base&#x201d; location was determined for each seep; locations were exported as a point shapefile.</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption>
<p>Plan and 3D view of water-column acoustic flares collected on the TAN1806 survey overlain Calypso HVF bathymetry (colored) and regional Bay of Plenty bathymetry (greyscale). Plan view show seep base locations (orange points) and fault lines (black lines) from <xref ref-type="bibr" rid="B46">Litchfield et&#x20;al. (2013)</xref>.</p>
</caption>
<graphic xlink:href="feart-09-785396-g002.tif"/>
</fig>
</sec>
<sec id="s2-4">
<title>Bathymetric Derivatives</title>
<p>Bathymetric derivatives were calculated in the ArcGIS spatial analysis toolbox Benthic Terrain Modeler (BTM; v3.0) (<xref ref-type="bibr" rid="B80">Wright et&#x20;al., 2012</xref>; <xref ref-type="bibr" rid="B78">Walbridge et&#x20;al., 2018</xref>). Derivative layers were created using a standard 3&#x20;&#xd7; 3 neighborhood window. All bathymetric derivatives are described in <xref ref-type="table" rid="T1">Table&#x20;1</xref>.</p>
<table-wrap id="T1" position="float">
<label>TABLE 1</label>
<caption>
<p>Bathymetric and backscatter derivatives generated for modelling. All bathymetric derivatives are calculated using a moving 3 &#xd7; 3 window (Moore&#x2019;s neighborhood).</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Derivative</th>
<th align="center">Description</th>
<th align="center">Unit</th>
<th align="center">Software</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">Bathymetry</td>
<td align="left">Water depth</td>
<td align="left">Meters</td>
<td align="left">Spatial Analyst&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Slope</td>
<td align="left">The gradient or &#x201c;rate-of-maximum change&#x201d; in degree units. Calculated according to Horn&#x2019;s algorithm</td>
<td align="left">Degrees (0&#x2013;90&#xb0;)</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Curvature</td>
<td align="left">Slope of slope; derivative of &#x201c;rate-of-maximum change&#x201d;. Highlights landform boundaries (e.g. ridges) that influence current regimes</td>
<td align="left">Degrees per meter</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Profile curvature</td>
<td align="left">Curvature in the direction of slope. Highlights terraces and scarps</td>
<td align="left">Degrees per meter</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Plan curvature</td>
<td align="left">Curvature perpendicular to slope. Highlights flow lines</td>
<td align="left">Change of degrees per meter</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Broad bathymetric position index (bBPI)</td>
<td align="left">A measure of referenced location relative to the overall terrain that highlights broad scale highs and lows, e.g. Slopes and depressions. A modification of topographic position index (Weiss, 2001). Parameters: 50&#x2013;100&#xa0;m</td>
<td align="left">Unitless</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Fine bathymetric position index (fBPI)</td>
<td align="left">A measure of referenced location relative to the overall terrain that highlights fine scale highs and lows, e.g. Narrow crests, mid-slope depressions. A modification of topographic position index (Weiss, 2001). Parameters: 10&#x2013;50&#xa0;m</td>
<td align="left">Unitless</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Vector ruggedness measure (VRM)</td>
<td align="left">A measure of surface roughness/rugosity</td>
<td align="left">Unitless</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Aspect</td>
<td align="left">A measure of surface direction, clockwise from north</td>
<td align="left">Degrees (0&#x2013;359.9&#xb0;)</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Northerness</td>
<td align="left">A secondary measure of surface calculation, from due south to due north. Values range from 1 to &#x2212;1</td>
<td align="left">Unitless</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Easterness</td>
<td align="left">A secondary measure of surface calculation, from due west to due east. Values range from 1 to &#x2212;1</td>
<td align="left">Unitless</td>
<td align="left">BTM v3.0&#x2014;ArcGIS v10.5.1</td>
</tr>
<tr>
<td align="left">Brightness</td>
<td align="left">A measure of mean layer intensity (brightness) of a segment&#x2019;s pixel values and brightness in relation to another segment&#x2019;s pixel values</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Max. Difference</td>
<td align="left">A measure of the maximum difference in brightness (mean intensity) levels between two image layers</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Mean 200&#xa0;kHz</td>
<td align="left">Average backscatter of the EM 2040&#x20;200&#xa0;kHz mosaic</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Mean 30&#xa0;kHz</td>
<td align="left">Average backscatter of the EM302 30&#xa0;kHz mosaic</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Std-dev 200&#xa0;kHz</td>
<td align="left">Standard deviation of EM 2040&#x20;200&#xa0;kHz backscatter values</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Std-dev 30&#xa0;kHz</td>
<td align="left">Standard deviation of EM302 30&#xa0;kHz backscatter values</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Skew 200&#xa0;kHz</td>
<td align="left">Skewness of EM 2040&#x20;200&#xa0;kHz backscatter values</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">Skew 20&#xa0;kHz</td>
<td align="left">Skewness of EM302 30&#xa0;kHz backscatter values</td>
<td align="left">Decibels</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM homogeneity</td>
<td align="left">A measure of closeness of the distribution of elements in the GLCM diagonal</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM contrast</td>
<td align="left">A measure of intensity contrast between a pixel and its neighbor over the entire image; a &#x201c;sum of squares variance&#x201d; GLCM contrast measures the amount of local variation in an image. Opposite to GLCM homogeneity. Weights increase exponentially</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM dissimilarity</td>
<td align="left">A measure like contrast but increases linearly. High if the local region has high contrast</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM entropy</td>
<td align="left">Measures high if the elements in the GLCM are equally distributed; measure low if the elements of the GLCM are closer to 0 or 1</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM angular second moment</td>
<td align="left">A measure of local homogeneity; high if some elements are large and the remaining elements are small</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM mean</td>
<td align="left">A measure of the average expressed in terms of the GLCM. Each pixel value is weighted by the frequency of its co-occurrence with a neighbor pixel value</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM standard deviation</td>
<td align="left">A measure of the standard deviation expressed in terms of the GLCM calculated by the co-occurrence of reference and neighbor pixel values</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
<tr>
<td align="left">GLCM correlation</td>
<td align="left">A measure of linear dependency of a reference pixel to a neighbor pixel</td>
<td align="left">Unitless</td>
<td align="left">Trimble eCognition v9.4</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s2-5">
<title>Seafloor Backscatter</title>
<p>MBES seafloor backscatter data were imported into SonarScope software (IFREMER, France; <xref ref-type="bibr" rid="B2">Augustin (2016)</xref>). Bathymetric sounding validity flags from Caris HIPS/SIPS were applied to corresponding backscatter samples. Kongsberg backscatter corrections applied at time of recording were removed while compensation for local backscatter angular variation and a transmit beam pattern were applied to ensure precise data reduction (<xref ref-type="bibr" rid="B52">Lurton and Lamarche, 2015</xref>). Very high overlap of MBES lines during the survey allowed only beams outside nadir (-20&#xb0;/20&#xb0;) to be considered, with an average backscatter (decibel, dB) value calculated from multiple samples for each grid point. Backscatter mosaics of both frequencies (200 and 30&#xa0;kHz) were produced at 2&#xa0;m resolution (<xref ref-type="fig" rid="F3">Figure&#x20;3</xref>).</p>
<fig id="F3" position="float">
<label>FIGURE 3</label>
<caption>
<p>Original Calypso HVF EM 2040 (200&#xa0;kHz) backscatter mosaic <bold>(A)</bold>, and Calypso HVF EM302 (30&#xa0;kHz) backscatter mosaic <bold>(B)</bold>, <bold>(C)</bold>, <bold>(D)</bold>, <bold>(E)</bold>, <bold>(F)</bold>, <bold>(G)</bold>, <bold>(H)</bold>, <bold>(I)</bold>, <bold>(J)</bold>, <bold>(K)</bold>, <bold>(L)</bold>, <bold>(M)</bold>, <bold>(N)</bold>, <bold>(O)</bold>, <bold>(P)</bold> insets show detailed comparisons between the two backscatter mosaics. High backscatter intensities (high reflectivity &#x3d; harder substrate) are lighter; low backscatter intensities (low reflectivity &#x3d; softer substrates) are darker. Blue lines are 50&#xa0;m contours; mosaic resolution 2&#xa0;m; projection NZTM. Very dark areas (black) in the 30&#xa0;kHz mosaic show acquisition artefacts from aeration of the transducer due to rough sea conditions. Although original mosaic ranges shown include extreme outliers, color ramps are normalized here to ensure comparable grey tones. Mosaic distributions predominantly lie within &#x2212;30&#x2013;&#x2212;5&#xa0;dB.</p>
</caption>
<graphic xlink:href="feart-09-785396-g003.tif"/>
</fig>
<p>An averaging filter (3 &#xd7; 3 moving window) was passed over the backscatter mosaic to interpolate small pixel-wide gaps from missing data. Both mosaics were clipped from &#x2212;30&#xa0;dB to &#x2212;5 dB to remove large areas of no data and mosaic artefacts (outliers). Both mosaics were classified individually, following Jenks Natural Breaks method (<xref ref-type="bibr" rid="B33">Jenks, 1967</xref>) to highlight comparable and dissimilar backscatter classes within each mosaic (<xref ref-type="fig" rid="F4">Figure&#x20;4</xref>). Jenks method sorts values into natural classes by reducing the variance within classes while maximizing variance between classes (<xref ref-type="bibr" rid="B33">Jenks, 1967</xref>).</p>
<fig id="F4" position="float">
<label>FIGURE 4</label>
<caption>
<p>Sediment grab results and Jenks Natural Breaks backscatter classes for <bold>(A)</bold> Calypso HVF 200&#xa0;kHz mosaic, and <bold>(B)</bold> Calypso HVF 30&#xa0;kHz mosaic. Grab results show sand-dominant classes in the North and silt-dominant classes in the South (grey lines: 50&#xa0;m contours; black dots: seep base locations; mosaic resolution: 2&#xa0;m; projection: NZTM). Inset of 200&#xa0;kHz <bold>(C)</bold> and of 30&#xa0;kHz <bold>(D</bold>) show two depressions in North Calypso HVF, with grab samples within each classified as coarse or very coarse&#x20;silt.</p>
</caption>
<graphic xlink:href="feart-09-785396-g004.tif"/>
</fig>
</sec>
<sec id="s2-6">
<title>Segmentation of Seafloor Acoustic Data</title>
<p>Backscatter mosaics were segmented using Geographic Object Based Image Analysis (GEOBIA) to identify homogenous regions of seafloor for input into a random forest model. This was achieved using a multiresolution segmentation algorithm in eCognition software (v9.4). Three mosaic configurations were segmented, with bathymetry incorporated in each: 1) 200&#xa0;kHz backscatter only, 2) 30&#xa0;kHz backscatter only, and 3) equally weighted EM2040 and EM302 backscatter. Three regions were segmented: the full Calypso HVF dataset, the North Calypso HVF subset, and the South Calypso HVF subset. The individual Calypso HVF regions were analyzed separately to: 1) account for the large differences in acoustic response observed between the north and south (<xref ref-type="fig" rid="F3">Figure&#x20;3</xref>); 2) remove the influence of poor weather acquisition artefacts in the North Calypso HVF 30&#xa0;kHz mosaic (<xref ref-type="fig" rid="F3">Figure&#x20;3B</xref>); and 3) account for the historic classification of the Calypso HVF&#x2014;the North Calypso HVF region includes the data described by <xref ref-type="bibr" rid="B16">Duncan and Pantin (1969)</xref>, while the South Calypso HVF includes the South flare areas, described by <xref ref-type="bibr" rid="B75">Stoffers et&#x20;al. (1999)</xref> and <xref ref-type="bibr" rid="B6">Botz et&#x20;al. (2002)</xref>.</p>
<p>Each mosaic configuration and region were segmented using seven different scale parameters (SPs): 9, 14, 26, 46, 50, 75, and 100. The Estimation of Scale Parameter tool (ESP2) (<xref ref-type="bibr" rid="B15">Dragut et&#x20;al., 2010</xref>) was used to determine the appropriate SP for analysis. The SP is a unitless term to describe the minimum size of the segments. SP levels of 9, 14, 26, and 46 were selected automatically using ESP2. Larger, manually selected SPs (50, 75, and 100) were also interrogated to explore differences between auto- and manually selected SPs. We selected segmentation settings that emphasize the influence of color (greyscale color heterogeneity, in the case of backscatter mosaics) rather than shape (shape &#x3d; 0.1, color &#x3d; 0.9). For eCognition, shape and color parameters influence each other (high influence of color equals low influence of shape, and vice versa). Compactness (border length/area) and smoothness of the boundary were equally weighted (0.5) with the aim of extracting backscatter areas with a high color contrast to surrounding seafloor (i.e. &#x201c;hardgrounds&#x201d;). Segments along the immediate perimeter of the survey area were removed to avoid edge effects created from backscatter processing.</p>
<p>Similar to the original Jenks classification of individual mosaics (pixel-based classification), segments were classified into five classes based on mean backscatter intensity values (derived from all pixels that contribute to a segment), following Jenks Natural Breaks method (<xref ref-type="bibr" rid="B33">Jenks, 1967</xref>) (object-based classification). The five classes reflect the mean backscatter values for each segment from lowest to highest values and each class capturing internally homogenous areas of seafloor (<xref ref-type="fig" rid="F6">Figure&#x20;6</xref>).</p>
</sec>
<sec id="s2-7">
<title>Textural Analysis of Backscatter Layers</title>
<p>Statistical attributes were generated for each segment, including brightness (combined intensity of both mosaics), maximum difference (backscatter dB difference), and the mean, standard deviation, and skewness of both the 200 and 30&#xa0;kHz mosaics (<xref ref-type="table" rid="T1">Table&#x20;1</xref>). These values are calculated from all pixel backscatter dB levels that construct each individual image segment. Each segment produces one value for each measure.</p>
<p>Textural values were also generated for each segment in eCognition using the Haralick grey level co-occurrence matrix (GLCM) (<xref ref-type="bibr" rid="B26">Haralick et&#x20;al., 1973</xref>). Texture metrics are commonly used to identify differences between seafloor substrates or associated habitats and can be used as input layers for seafloor classification models (<xref ref-type="bibr" rid="B51">Lucieer, 2008</xref>; <xref ref-type="bibr" rid="B11">Buscombe, 2017</xref>). Specific to hardground and fluid expulsion in a shallow hydrothermal system, GLCM texture variables provide high-resolution seafloor characteristics in areas where large topographic changes are limited (i.e. flat seafloor). Here, we used the invariant direction, which is the sum of values for four spatial directions (0&#xb0;, 45&#xb0;, 90&#xb0;, and 135&#xb0;), for the two mosaics individually and then combined. The GLCM analyses relationships between two pixels at a time, a reference and neighbor pixel, using an offset of 1 pixel (i.e. direct neighbor pixels). All backscatter derivative descriptions are provided in <xref ref-type="table" rid="T1">Table&#x20;1</xref>.</p>
<p>Each segmentation layer (for each mosaic configuration and SP) was exported as a shapefile with all textural and geomorphic values attached to each segment. The segment layers and a point shapefile of flare locations (from <xref ref-type="sec" rid="s3-4">Section 3.4</xref>) were linked in ArcGIS. Segments that were co-located with flare locations were classed as &#x201c;active&#x201d; segments and those without flare locations were classed as &#x201c;inactive.&#x201d; This process created attribute tables for each segmentation layer with each unique underlying textural and geomorphic values for all &#x2018;active&#x2019; and &#x201c;inactive&#x201d; segments. Sixty-three joined attribute files were exported as a. csv file for further analysis.</p>
</sec>
<sec id="s2-8">
<title>Random Forest Classification</title>
<p>The segment attribute files exported in <xref ref-type="sec" rid="s3-6">Section 3.6</xref> were used as input data for random forest (RF) classification. The data encompass seven different SP (9, 14, 26, 46, 50, 75, and 100), three layers from different frequencies (30&#xa0;kHz mosaic, 200&#xa0;kHz mosaic, and a combined mosaic (equally weighted) of the two frequencies) and three regions (the full Calypso HVF dataset, the North Calypso HVF subset, and the South Calypso HVF subset).</p>
<p>Supervised RF models were run on each individual exported attribute file, executed in the &#x201c;randomForest&#x201d; R package (<xref ref-type="bibr" rid="B44">Liaw and Weiner, 2002</xref>). The RF algorithm trains an ensemble of decision trees by randomly selecting and testing a random subset of predictor variables at each node and then pooling all tree results (<xref ref-type="bibr" rid="B8">Brieman, 2001</xref>). The RF algorithm was chosen due to its ability to deal with unbalanced classes (as is the case with &#x201c;active&#x201d; and &#x201c;inactive&#x201d; seafloor segments (<xref ref-type="bibr" rid="B55">Mellor et&#x20;al., 2015</xref>) and its robustness against over-fitting (<xref ref-type="bibr" rid="B50">Lucieer et&#x20;al., 2012</xref>; <xref ref-type="bibr" rid="B10">Buscombe and Grams, 2018</xref>). The data sets were randomly split into two groups&#x2014;training data (70%) for model development and test data (30%) for model evaluation. Binary &#x201c;presence/absence&#x201d; (i.e. &#x201c;active&#x201d; vs &#x201c;inactive&#x201d;) classes were the target. Attribute values from each segment were the predictor variables: backscatter mean, standard deviation, and skewness; backscatter derivatives (GLCM textures), water depth, and bathymetric derivatives (BTM layers). Each model ran with 500 trees and the square root of the number of predictor variables as the subset at each node (rounded down).</p>
<p>Error matrices and model performance statistics (Kappa, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV)) were calculated for each model. Error matrices compare known class data to predicted classes in a 2&#x20;&#xd7; 2 table (true positive, true negative, false positive, and false negative). Model &#x201c;goodness&#x201d; thresholds were determined by Kappa values. This value allows for model comparison by quantifying the accuracy of each model above that of chance: 0&#x20;&#x3d; predictive power no better than chance, 0&#x2013;0.2 &#x3d; poor, 0.2&#x2013;0.4 &#x3d; fair, 0.4&#x2013;0.6 &#x3d; moderate, 0.6&#x2013;0.8 &#x3d; good and 0.8&#x2013;1 &#x3d; very good, 1&#x20;&#x3d; perfect predictive power (<xref ref-type="bibr" rid="B1">Altman, 1991</xref>). Sensitivity conveys the ratio of true positive predictions (flare presence) to the total positive observations (i.e. number of correct seep predictions to the number of actual seeps). Specificity conveys to the ratio of true negative predictions (seep absence) to the total negative observations. PPV conveys the ratio of true presence predictions to the total positive predictions (i.e. number of correct seep predictions to the number of positive predictions). NPV denotes the ratio of true negative predictions to the total negative predictions. A &#x201c;Mean Decrease Accuracy&#x201d; measure is also generated for each model. This measure describes the relative importance of each predictor variable, by determining how much a model&#x2019;s accuracy degrades if a specific variable is excluded from the model. Variables with high importance drive the overall prediction outcome of an RF&#x20;model.</p>
</sec>
<sec id="s2-9">
<title>Sediment Samples</title>
<p>Thirty-one Van Veen sediment grabs were collected over the survey region, 14 in North Calypso HVF, eight in South Calypso HVF, and nine in the remaining Calypso HVF region (<xref ref-type="fig" rid="F1">Figure&#x20;1B</xref>). A Van Veen sediment grab collected surface sediments, representing the top 30&#xa0;cm of the seabed. Grain size distributions were extracted using a Beckman Coulter LS13 320 Diffraction Particle Size Analyzer (DPSA). Results were analyzed using Gratistat v8 (<xref ref-type="bibr" rid="B4">Blott and Pye, 2001</xref>). Calcium carbonate content was determined using a vacuum-gasometric system (<xref ref-type="bibr" rid="B34">Jones and Kaiteris, 1983</xref>). Particle size distributions and D<sub>50</sub> (median) were measured and reported in log transformation units (<italic>&#x3d5;</italic>) (<xref ref-type="bibr" rid="B4">Blott and Pye, 2001</xref>). Samples were classified into one of six descriptive sediment classes: medium silt, coarse silt, very coarse silt, very fine sand, fine sand, medium sand (<xref ref-type="bibr" rid="B19">Folk and Ward, 1957</xref>).</p>
</sec>
<sec id="s2-10">
<title>Towed Camera</title>
<p>Ten towed camera transects were sampled over three flares-of-interest (FOI) (FOI-01, -02, and -03; <xref ref-type="fig" rid="F1">Figure&#x20;1B</xref> white boxes), where high intensity water-column acoustic flare activity was observed during the survey. A towed video camera system recorded continuous digital color video footage (Sony HDR-CX550; Institute for Marine and Antarctic Studies). The video camera was weighted and mounted in a &#x201c;fly frame&#x201d; to ensure a consistent altitude above the seafloor. The camera was angled at 45&#xb0; to capture gas bubble and fluid release from the seafloor, with lasers spaced at 15&#xa0;cm for a scale reference. Additional GoPro cameras were attached to the towed camera system for some of the camera transects, affording multiple perspectives of the seafloor.</p>
<p>A USBL transponder was attached to the camera deployment line (&#x223c;0.5&#xa0;m upline from camera attachment point) and linked to the RV <italic>Tangaroa</italic> Kongsberg HiPAP system. Transects were towed at ship speeds between 0.25 and 1.0&#xa0;m&#xa0;s<sup>&#x2212;1</sup>, with the aim of maintaining an altitude of &#x223c;1&#x2013;2&#xa0;m and a visible area of&#x20;&#x223c;1.5&#x2013;2.5&#xa0;m<sup>2</sup>. A total of 17&#xa0;h of towed camera and 1,127 still images were obtained over the ten transects, capturing 12&#xa0;km of seafloor.</p>
</sec>
</sec>
<sec sec-type="results" id="s3">
<title>Results</title>
<sec id="s3-1">
<title>Active Seafloor Fluid Expulsion Areas</title>
<p>A total of 3,222 individual seeps were identified over the mapped 115&#xa0;km<sup>2</sup> Calypso HVF, between water depths of 111&#x2013;292&#xa0;m (<xref ref-type="fig" rid="F1">Figures 1</xref>, <xref ref-type="fig" rid="F2">2</xref>). The seeps occupy &#x223c;9&#xa0;km<sup>2</sup> of seafloor, &#x223c;8% of the Calypso HVF, with the remaining 92% of seafloor observed as inactive (at the time of survey). Several primary areas of active fluid expulsion were identified that agree with previous records of activity (<xref ref-type="bibr" rid="B81">Wright, 1992</xref>; <xref ref-type="bibr" rid="B39">Lamarche and Barnes, 2005</xref>; <xref ref-type="bibr" rid="B28">Hocking et&#x20;al., 2010</xref>). These active areas occur either within or along the perimeter of ovoid or circular depressions that align with the visible NE-SW trending normal faults of Calypso 2, Pukehoko, and Nukuhou (<xref ref-type="bibr" rid="B46">Litchfield et&#x20;al., 2013</xref>). The main North Calypso HVF activity is observed as a constellation of seeps that skirt the perimeter of two &#x223c;1&#xa0;km wide ovoid depressions that overlay the Calypso 2 normal fault (<xref ref-type="fig" rid="F2">Figure&#x20;2</xref>). The South Calypso HVF activity occurs within distinct depressions that occur between 1 and 1.5&#xa0;km south of the Pukehoko North fault and between 0 and 1&#xa0;km north of the Nukuhou fault. Fluid expulsion activity within the center of the Calypso HVF, the transition zone between north and south, lies between the Calypso 2 and Pukehoko North faults (<xref ref-type="fig" rid="F2">Figure&#x20;2</xref>).</p>
</sec>
<sec id="s3-2">
<title>Seafloor Backscatter Response</title>
<p>The two frequencies used to map the Calypso HVF produce mosaics that have visible differences in seafloor backscatter intensity (<xref ref-type="fig" rid="F3">Figure&#x20;3</xref>). Backscatter intensity values of the original 200&#xa0;kHz mosaic range between &#x2212;46 and &#x2b;27&#xa0;dB while the intensity values of the original 30&#xa0;kHz mosaic range between &#x2212;92 and 0&#xa0;dB (<xref ref-type="fig" rid="F3">Figure&#x20;3</xref>). These ranges included extreme outliers due to acquisition artefact (<xref ref-type="fig" rid="F3">Figure&#x20;3</xref>). Mosaic distributions were clipped to &#x2212;30&#x2013;&#x2212;5&#xa0;dB (see <xref ref-type="sec" rid="s3-4">Section 3.4</xref> and <xref ref-type="fig" rid="F4">Figure&#x20;4</xref>). Differences between the two mosaics are most obvious in the North Calypso HVF when observed with the Jenks classification (<xref ref-type="fig" rid="F4">Figure&#x20;4</xref>). While very-high backscatter intensity patches (hardgrounds) are observed to largely correspond in both mosaics, the 200&#xa0;kHz mosaic has an overall higher backscatter intensity than the 30&#xa0;kHz mosaic in the vicinity of the North Calypso HVF seeps (<xref ref-type="fig" rid="F3">Figure&#x20;3C</xref> (200&#xa0;kHz), d (30&#xa0;kHz); <xref ref-type="fig" rid="F4">Figure&#x20;4A</xref>). The 30&#xa0;kHz mosaic also reveals more defined boundaries between hardgrounds and the surrounding seafloor while boundaries are more diffuse in the 200&#xa0;kHz mosaic (<xref ref-type="fig" rid="F3">Figures 3E,F</xref>, <xref ref-type="fig" rid="F4">4D</xref>).</p>
<p>In the North Calypso HVF, an extensive assemblage of hardgrounds skirts the perimeter of two ovoid depressions, coinciding with the primary seep area; these patches are more conspicuous in the 30&#xa0;kHz mosaic than the 200&#xa0;kHz mosaic (<xref ref-type="fig" rid="F3">Figures 3C,D</xref>, <xref ref-type="fig" rid="F4">4D</xref>). The ovoid depressions show an inverse backscatter relationship&#x2014;lower backscatter intensity in the 200&#xa0;kHz (lower intensity) and higher in the 30&#xa0;kHz (higher intensity). Two discrete very-high backscatter patches in the north-west (<xref ref-type="fig" rid="F3">Figures 3E,F</xref>) and west (<xref ref-type="fig" rid="F3">Figures 3G,H</xref>) largely correspond in both two mosaics.</p>
<p>In the South Calypso HVF, there are two large conspicuous patches (<xref ref-type="fig" rid="F3">Figures 3O,P</xref>, <xref ref-type="fig" rid="F4">4A</xref>) of very-high backscatter observable in both mosaics though more obvious in the 200&#xa0;kHz mosaic. Here, a very-high intensity ring is visible in the 200&#xa0;kHz but less obvious in the 30&#xa0;kHz. Another two smaller patches of backscatter are observed. One is visible in the 200&#xa0;kHz mosaic while almost absent in the 30&#xa0;kHz (<xref ref-type="fig" rid="F3">Figures 3M,N</xref>), while the second shows the inverse relationship (<xref ref-type="fig" rid="F3">Figures 3A,B</xref>). Very-high backscatter intensity patches are also observed to correspond to fault scarps in the center (<xref ref-type="fig" rid="F3">Figures 3K,L</xref>) and south (<xref ref-type="fig" rid="F3">Figures 3O,P</xref>) of the survey area. These linear shaped patterns align with the Pukehoko and Nukuhou normal faults (<xref ref-type="fig" rid="F2">Figure&#x20;2</xref>).</p>
</sec>
<sec id="s3-3">
<title>Seafloor Sediment</title>
<p>The demarcation in seafloor backscatter observed between the two sites along the central scarp region (<xref ref-type="fig" rid="F3">Figures 3A,B</xref>) is mirrored in the sediment grabs as a fining (decrease in median grain size) of sediments from north to south. The average D<sub>50</sub> (median <italic>&#x3d5;</italic>) particle size for North Calypso HVF was 3&#x20;<italic>&#x3d5;</italic> (125&#xa0;&#x3bc;m) and for South Calypso HVF was 6&#x20;<italic>&#x3d5;</italic> (16&#xa0;&#x3bc;m). As D<sub>50</sub> particle size decreases, the difference in backscatter intensity between the two different frequency mosaics also decreases (<xref ref-type="fig" rid="F5">Figure&#x20;5A</xref>). North Calypso HVF sediment grab samples exhibit a range of particle sizes from very fine sand to very coarse silt (<xref ref-type="table" rid="T2">Table&#x20;2</xref>; <xref ref-type="fig" rid="F4">Figures 4A,B</xref>). Of note are samples taken from within two circular depressions which are classified as coarse or very coarse silt due to their dominant silt fraction (64 and 75% weight) (<xref ref-type="fig" rid="F5">Figures 5A,B</xref>). The South Calypso HVF sediment grab samples reveal a silt-dominant substrate (<xref ref-type="fig" rid="F4">Figures 4A,B</xref>), with all samples classified as medium silt (<xref ref-type="table" rid="T2">Table&#x20;2</xref>).</p>
<fig id="F5" position="float">
<label>FIGURE 5</label>
<caption>
<p>
<bold>(A)</bold> 200 (blue circles) and 30 (orange triangles) kHz backscatter values and D50 (median &#x3d5;) particle size. All South Calypso HVF samples are highlighted within the black rectangle, where &#x3d5; &#x3e; 6.0 (smallest median particle sizes). Infilled icons indicate samples from the two depressions in the North Calypso HVF. <bold>(B)</bold> 200 (blue) and 30 (orange) kHz backscatter intensity and Folk &#x26; Ward sediment classification. All South Calypso HVF samples are medium silt, highlighted within the black rectangle. Here, there is minimal overlap in range between 200 and 30&#xa0;kHz mosaic backscatter values.</p>
</caption>
<graphic xlink:href="feart-09-785396-g005.tif"/>
</fig>
<table-wrap id="T2" position="float">
<label>TABLE 2</label>
<caption>
<p>Sediment grab Folk and Ward classes, composition, and corresponding Jenks Natural Breaks acoustic classes for both mosaics.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Region</th>
<th align="center">Sample ID</th>
<th align="center">Lon.</th>
<th align="center">Lat.</th>
<th align="center">Folk &#x26; ward</th>
<th align="center">Sand %</th>
<th align="center">Silt %</th>
<th align="center">Clay %</th>
<th align="center">Carb. %</th>
<th align="center">D<sub>50</sub> (&#x3a6;)</th>
<th align="center">Mean diam. (&#x3a6;)</th>
<th align="center">Sort. (&#x3a6;)</th>
<th align="center">Skew. (&#x3a6;)</th>
<th align="center">dB 200&#xa0;kHz</th>
<th align="center">dB 30&#xa0;kHz</th>
<th align="center">Depth</th>
<th align="center">Jenks 200&#xa0;kHz</th>
<th align="center">Jenks 30&#xa0;kHz</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">32</td>
<td align="char" char=".">177.0693</td>
<td align="char" char=".">&#x2212;37.6540</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.69</td>
<td align="char" char=".">0.30</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">2.00</td>
<td align="char" char=".">2.14</td>
<td align="char" char=".">3.16</td>
<td align="char" char=".">2.55</td>
<td align="char" char=".">0.56</td>
<td align="char" char=".">&#x2212;17.06</td>
<td align="char" char=".">&#x2212;28.15</td>
<td align="char" char=".">&#x2212;134.7</td>
<td align="left">Medium</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">33</td>
<td align="char" char=".">177.0755</td>
<td align="char" char=".">&#x2212;37.6601</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.52</td>
<td align="char" char=".">0.47</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">3.22</td>
<td align="char" char=".">3.59</td>
<td align="char" char=".">3.99</td>
<td align="char" char=".">2.64</td>
<td align="char" char=".">0.20</td>
<td align="char" char=".">&#x2212;16.78</td>
<td align="char" char=".">&#x2212;24.28</td>
<td align="char" char=".">&#x2212;159.2</td>
<td align="left">High</td>
<td align="left">Medium</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">35</td>
<td align="char" char=".">177.0801</td>
<td align="char" char=".">&#x2212;37.6373</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.74</td>
<td align="char" char=".">0.25</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">2.89</td>
<td align="char" char=".">2.14</td>
<td align="char" char=".">2.95</td>
<td align="char" char=".">2.47</td>
<td align="char" char=".">0.51</td>
<td align="char" char=".">&#x2212;15.18</td>
<td align="center">nan</td>
<td align="char" char=".">&#x2212;142.1</td>
<td align="left">High</td>
<td align="left">FALSE</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">51</td>
<td align="char" char=".">177.1108</td>
<td align="char" char=".">&#x2212;37.6322</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.65</td>
<td align="char" char=".">0.34</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">1.86</td>
<td align="char" char=".">2.67</td>
<td align="char" char=".">3.48</td>
<td align="char" char=".">2.51</td>
<td align="char" char=".">0.43</td>
<td align="char" char=".">&#x2212;17.56</td>
<td align="char" char=".">&#x2212;28.23</td>
<td align="char" char=".">&#x2212;168.4</td>
<td align="left">Medium</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">92</td>
<td align="char" char=".">177.1453</td>
<td align="char" char=".">&#x2212;37.6137</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.58</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">3.50</td>
<td align="char" char=".">3.20</td>
<td align="char" char=".">3.88</td>
<td align="char" char=".">2.58</td>
<td align="char" char=".">0.33</td>
<td align="char" char=".">&#x2212;15.93</td>
<td align="char" char=".">&#x2212;29.07</td>
<td align="char" char=".">&#x2212;216.1</td>
<td align="left">High</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">37</td>
<td align="char" char=".">177.0624</td>
<td align="char" char=".">&#x2212;37.6294</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.72</td>
<td align="char" char=".">0.28</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">3.14</td>
<td align="char" char=".">2.25</td>
<td align="char" char=".">3.13</td>
<td align="char" char=".">2.53</td>
<td align="char" char=".">0.52</td>
<td align="char" char=".">&#x2212;15.94</td>
<td align="char" char=".">&#x2212;26.57</td>
<td align="char" char=".">&#x2212;132.8</td>
<td align="left">High</td>
<td align="left">Low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">43</td>
<td align="char" char=".">177.1032</td>
<td align="char" char=".">&#x2212;37.6008</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.27</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">3.76</td>
<td align="char" char=".">2.08</td>
<td align="char" char=".">2.96</td>
<td align="char" char=".">2.50</td>
<td align="char" char=".">0.52</td>
<td align="char" char=".">&#x2212;15.62</td>
<td align="char" char=".">&#x2212;28.81</td>
<td align="char" char=".">&#x2212;175.7</td>
<td align="left">High</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">87</td>
<td align="char" char=".">177.0774</td>
<td align="char" char=".">&#x2212;37.6140</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.71</td>
<td align="char" char=".">0.29</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">1.62</td>
<td align="char" char=".">2.47</td>
<td align="char" char=".">3.23</td>
<td align="char" char=".">2.33</td>
<td align="char" char=".">0.48</td>
<td align="char" char=".">&#x2212;14.40</td>
<td align="char" char=".">&#x2212;28.58</td>
<td align="char" char=".">&#x2212;148.8</td>
<td align="left">Very high</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">88</td>
<td align="char" char=".">177.0772</td>
<td align="char" char=".">&#x2212;37.6143</td>
<td align="left">Very Fine Sand</td>
<td align="char" char=".">0.60</td>
<td align="char" char=".">0.40</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">0.65</td>
<td align="char" char=".">2.95</td>
<td align="char" char=".">3.81</td>
<td align="char" char=".">2.53</td>
<td align="char" char=".">0.40</td>
<td align="char" char=".">&#x2212;16.23</td>
<td align="char" char=".">&#x2212;26.68</td>
<td align="char" char=".">&#x2212;148.4</td>
<td align="left">High</td>
<td align="left">Low</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">34</td>
<td align="char" char=".">177.0837</td>
<td align="char" char=".">&#x2212;37.6544</td>
<td align="left">Fine Sand</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.21</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">1.60</td>
<td align="char" char=".">1.97</td>
<td align="char" char=".">2.69</td>
<td align="char" char=".">2.18</td>
<td align="char" char=".">0.56</td>
<td align="char" char=".">&#x2212;16.37</td>
<td align="char" char=".">&#x2212;21.67</td>
<td align="char" char=".">&#x2212;146.2</td>
<td align="left">High</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">85</td>
<td align="char" char=".">177.0320</td>
<td align="char" char=".">&#x2212;37.6626</td>
<td align="left">Fine Sand</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.26</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">1.77</td>
<td align="char" char=".">2.79</td>
<td align="char" char=".">2.63</td>
<td align="char" char=".">0.59</td>
<td align="char" char=".">&#x2212;16.67</td>
<td align="char" char=".">&#x2212;25.57</td>
<td align="char" char=".">&#x2212;120.1</td>
<td align="left">High</td>
<td align="left">Low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">42</td>
<td align="char" char=".">177.1032</td>
<td align="char" char=".">&#x2212;37.6010</td>
<td align="left">Fine Sand</td>
<td align="char" char=".">0.81</td>
<td align="char" char=".">0.19</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">3.33</td>
<td align="char" char=".">1.69</td>
<td align="char" char=".">2.40</td>
<td align="char" char=".">2.33</td>
<td align="char" char=".">0.52</td>
<td align="char" char=".">&#x2212;16.39</td>
<td align="char" char=".">&#x2212;26.94</td>
<td align="char" char=".">&#x2212;175.3</td>
<td align="left">High</td>
<td align="left">Low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">45</td>
<td align="char" char=".">177.0931</td>
<td align="char" char=".">&#x2212;37.6102</td>
<td align="left">Fine Sand</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.18</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">2.01</td>
<td align="char" char=".">1.91</td>
<td align="char" char=".">2.59</td>
<td align="char" char=".">2.06</td>
<td align="char" char=".">0.54</td>
<td align="char" char=".">&#x2212;17.19</td>
<td align="char" char=".">&#x2212;28.56</td>
<td align="char" char=".">&#x2212;165.3</td>
<td align="left">Medium</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">90</td>
<td align="char" char=".">177.1189</td>
<td align="char" char=".">&#x2212;37.5963</td>
<td align="left">Fine Sand</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.22</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">4.25</td>
<td align="char" char=".">2.11</td>
<td align="char" char=".">2.85</td>
<td align="char" char=".">2.33</td>
<td align="char" char=".">0.51</td>
<td align="char" char=".">&#x2212;13.51</td>
<td align="char" char=".">&#x2212;24.78</td>
<td align="char" char=".">&#x2212;214.2</td>
<td align="left">Very high</td>
<td align="left">Medium</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">36</td>
<td align="char" char=".">177.0944</td>
<td align="char" char=".">&#x2212;37.6306</td>
<td align="left">Medium Sand</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.09</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">1.37</td>
<td align="char" char=".">1.43</td>
<td align="char" char=".">1.66</td>
<td align="char" char=".">1.79</td>
<td align="char" char=".">0.16</td>
<td align="char" char=".">&#x2212;15.23</td>
<td align="char" char=".">&#x2212;28.87</td>
<td align="char" char=".">&#x2212;163.9</td>
<td align="left">High</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">63</td>
<td align="char" char=".">177.1117</td>
<td align="char" char=".">&#x2212;37.6648</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.03</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.04</td>
<td align="char" char=".">5.56</td>
<td align="char" char=".">6.43</td>
<td align="char" char=".">6.48</td>
<td align="char" char=".">1.51</td>
<td align="char" char=".">0.05</td>
<td align="char" char=".">&#x2212;18.12</td>
<td align="char" char=".">&#x2212;25.02</td>
<td align="char" char=".">&#x2212;169.5</td>
<td align="left">Medium</td>
<td align="left">Medium</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">64</td>
<td align="char" char=".">177.0853</td>
<td align="char" char=".">&#x2212;37.6825</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.04</td>
<td align="char" char=".">0.94</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">3.82</td>
<td align="char" char=".">6.35</td>
<td align="char" char=".">6.34</td>
<td align="char" char=".">1.41</td>
<td align="char" char=".">&#x2212;0.01</td>
<td align="char" char=".">&#x2212;18.07</td>
<td align="char" char=".">&#x2212;22.53</td>
<td align="char" char=".">&#x2212;175.7</td>
<td align="left">Medium</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">77</td>
<td align="char" char=".">177.1321</td>
<td align="char" char=".">&#x2212;37.6872</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.07</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">4.43</td>
<td align="char" char=".">6.24</td>
<td align="char" char=".">6.21</td>
<td align="char" char=".">1.55</td>
<td align="char" char=".">-0.02</td>
<td align="char" char=".">&#x2212;19.36</td>
<td align="char" char=".">&#x2212;23.23</td>
<td align="char" char=".">&#x2212;195.8</td>
<td align="left">Low</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">78</td>
<td align="char" char=".">177.1333</td>
<td align="char" char=".">&#x2212;37.6821</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.04</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.03</td>
<td align="char" char=".">5.40</td>
<td align="char" char=".">6.40</td>
<td align="char" char=".">6.41</td>
<td align="char" char=".">1.50</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">&#x2212;20.47</td>
<td align="char" char=".">&#x2212;20.80</td>
<td align="char" char=".">&#x2212;196.5</td>
<td align="left">Low</td>
<td align="left">Very high</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">79</td>
<td align="char" char=".">177.1136</td>
<td align="char" char=".">&#x2212;37.6944</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.05</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">5.41</td>
<td align="char" char=".">6.38</td>
<td align="char" char=".">6.35</td>
<td align="char" char=".">1.46</td>
<td align="char" char=".">-0.02</td>
<td align="char" char=".">&#x2212;20.59</td>
<td align="char" char=".">&#x2212;21.95</td>
<td align="char" char=".">&#x2212;177.0</td>
<td align="left">Low</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">81</td>
<td align="char" char=".">177.0972</td>
<td align="char" char=".">&#x2212;37.6946</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.14</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.03</td>
<td align="char" char=".">4.28</td>
<td align="char" char=".">6.26</td>
<td align="char" char=".">6.09</td>
<td align="char" char=".">1.87</td>
<td align="char" char=".">&#x2212;0.13</td>
<td align="char" char=".">&#x2212;17.14</td>
<td align="char" char=".">&#x2212;28.56</td>
<td align="char" char=".">&#x2212;190.2</td>
<td align="left">Medium</td>
<td align="left">Very low</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">82</td>
<td align="char" char=".">177.0637</td>
<td align="char" char=".">&#x2212;37.6868</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.03</td>
<td align="char" char=".">0.95</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">5.12</td>
<td align="char" char=".">6.37</td>
<td align="char" char=".">6.38</td>
<td align="char" char=".">1.40</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">&#x2212;20.89</td>
<td align="char" char=".">&#x2212;22.23</td>
<td align="char" char=".">&#x2212;156.8</td>
<td align="left">Low</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">SCHVF</td>
<td align="char" char=".">83</td>
<td align="char" char=".">177.0659</td>
<td align="char" char=".">&#x2212;37.6742</td>
<td align="left">Medium Silt</td>
<td align="char" char=".">0.05</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">4.92</td>
<td align="char" char=".">6.31</td>
<td align="char" char=".">6.31</td>
<td align="char" char=".">1.48</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">&#x2212;18.53</td>
<td align="char" char=".">&#x2212;20.44</td>
<td align="char" char=".">&#x2212;156.5</td>
<td align="left">Medium</td>
<td align="left">Very high</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">52</td>
<td align="char" char=".">177.1191</td>
<td align="char" char=".">&#x2212;37.6128</td>
<td align="left">Coarse Silt</td>
<td align="char" char=".">0.24</td>
<td align="char" char=".">0.75</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">4.69</td>
<td align="char" char=".">5.94</td>
<td align="char" char=".">5.56</td>
<td align="char" char=".">2.12</td>
<td align="char" char=".">&#x2212;0.23</td>
<td align="char" char=".">&#x2212;17.78</td>
<td align="char" char=".">&#x2212;23.70</td>
<td align="char" char=".">&#x2212;191.3</td>
<td align="left">Medium</td>
<td align="left">Medium</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">91</td>
<td align="char" char=".">177.1336</td>
<td align="char" char=".">&#x2212;37.5984</td>
<td align="left">Coarse Silt</td>
<td align="char" char=".">0.30</td>
<td align="char" char=".">0.68</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">3.74</td>
<td align="char" char=".">5.42</td>
<td align="char" char=".">5.27</td>
<td align="char" char=".">2.35</td>
<td align="char" char=".">&#x2212;0.11</td>
<td align="char" char=".">&#x2212;17.32</td>
<td align="char" char=".">&#x2212;21.31</td>
<td align="char" char=".">&#x2212;232.5</td>
<td align="left">Medium</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">109</td>
<td align="char" char=".">177.1025</td>
<td align="char" char=".">&#x2212;37.6223</td>
<td align="left">Coarse Silt</td>
<td align="char" char=".">0.34</td>
<td align="char" char=".">0.64</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">6.60</td>
<td align="char" char=".">5.19</td>
<td align="char" char=".">5.21</td>
<td align="char" char=".">2.10</td>
<td align="char" char=".">0.03</td>
<td align="char" char=".">&#x2212;17.54</td>
<td align="char" char=".">&#x2212;21.19</td>
<td align="char" char=".">&#x2212;182.8</td>
<td align="left">Medium</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">84</td>
<td align="char" char=".">177.0478</td>
<td align="char" char=".">&#x2212;37.6635</td>
<td align="left">Very Coarse Silt</td>
<td align="char" char=".">0.47</td>
<td align="char" char=".">0.52</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">3.80</td>
<td align="char" char=".">4.38</td>
<td align="char" char=".">4.23</td>
<td align="char" char=".">2.70</td>
<td align="char" char=".">&#x2212;0.03</td>
<td align="char" char=".">&#x2212;16.25</td>
<td align="center">nan</td>
<td align="char" char=".">&#x2212;135.6</td>
<td align="left">High</td>
<td align="left">FALSE</td>
</tr>
<tr>
<td align="left">CHVF</td>
<td align="char" char=".">93</td>
<td align="char" char=".">177.1015</td>
<td align="char" char=".">&#x2212;37.6391</td>
<td align="left">Very Coarse Silt</td>
<td align="char" char=".">0.40</td>
<td align="char" char=".">0.59</td>
<td align="char" char=".">0.00</td>
<td align="char" char=".">3.30</td>
<td align="char" char=".">4.93</td>
<td align="char" char=".">4.78</td>
<td align="char" char=".">2.18</td>
<td align="char" char=".">&#x2212;0.07</td>
<td align="char" char=".">&#x2212;17.48</td>
<td align="char" char=".">&#x2212;23.86</td>
<td align="char" char=".">&#x2212;172.4</td>
<td align="left">Medium</td>
<td align="left">Medium</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">49</td>
<td align="char" char=".">177.1009</td>
<td align="char" char=".">&#x2212;37.6226</td>
<td align="left">Very Coarse Silt</td>
<td align="char" char=".">0.62</td>
<td align="char" char=".">0.37</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">1.71</td>
<td align="char" char=".">3.24</td>
<td align="char" char=".">4.09</td>
<td align="char" char=".">2.16</td>
<td align="char" char=".">0.50</td>
<td align="char" char=".">&#x2212;19.83</td>
<td align="char" char=".">&#x2212;22.37</td>
<td align="char" char=".">&#x2212;181.1</td>
<td align="left">Low</td>
<td align="left">High</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">55</td>
<td align="char" char=".">177.1179</td>
<td align="char" char=".">&#x2212;37.6091</td>
<td align="left">Very Coarse Silt</td>
<td align="char" char=".">0.53</td>
<td align="char" char=".">0.45</td>
<td align="char" char=".">0.02</td>
<td align="char" char=".">4.45</td>
<td align="char" char=".">3.59</td>
<td align="char" char=".">4.11</td>
<td align="char" char=".">2.65</td>
<td align="char" char=".">0.24</td>
<td align="char" char=".">&#x2212;16.03</td>
<td align="center">-26.84</td>
<td align="char" char=".">&#x2212;187.5</td>
<td align="left">High</td>
<td align="left">Low</td>
</tr>
<tr>
<td align="left">NCHVF</td>
<td align="char" char=".">89</td>
<td align="char" char=".">177.1042</td>
<td align="char" char=".">&#x2212;37.5882</td>
<td align="left">Very Coarse Silt</td>
<td align="char" char=".">0.42</td>
<td align="char" char=".">0.57</td>
<td align="char" char=".">0.01</td>
<td align="char" char=".">4.21</td>
<td align="char" char=".">4.86</td>
<td align="char" char=".">4.66</td>
<td align="char" char=".">2.56</td>
<td align="char" char=".">&#x2212;0.07</td>
<td align="char" char=".">&#x2212;16.17</td>
<td align="char" char=".">&#x2212;25.36</td>
<td align="char" char=".">&#x2212;234.4</td>
<td align="left">High</td>
<td align="left">Medium</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s3-4">
<title>Backscatter Segmentation</title>
<p>All 200&#xa0;kHz-derived GEOBIA segments and most of the 30&#xa0;kHz-derived and combined mosaic GEOBIA segments have irregular shapes for all SPs, aligning with observed natural features in both the bathymetry and backscatter (subsets shown in <xref ref-type="fig" rid="F6">Figure&#x20;6</xref> show SP46 for the NCHVF and SP75 for the SCHVF, the two best performing models for each region). Segments that do not align with natural features within the 30&#xa0;kHz or combined segmentation are due to artefacts in the mosaics (i.e. predominantly artefacts that are a result of aeration of the transducer due to rough sea conditions).</p>
<fig id="F6" position="float">
<label>FIGURE 6</label>
<caption>
<p>Seeps (black dots), segmentation (white lines), and Jenks Natural Breaks re-classification of segments (colours) based on distribution of mean backscatter levels. Row names refer to location on inset map. First two rows <bold>(A-H)</bold> show North Calypso HVF subsets of the segmentation with SP46 (the best performing NCHVF model); bottom two rows <bold>(I-P)</bold> show South Calypso HVF subsets of the segmentation with SP75 (the best performing SCHVF model). First column <bold>(A,E,I,M)</bold> shows Jenks classes of mean 200&#xa0;kHz backscatter values. Second column <bold>(B,F,J,N)</bold> shows segment Jenks classes of mean 30&#xa0;kHz backscatter values. Third column <bold>(C,G,K,O)</bold> shows comparison of very-high backscatter segments of the 200&#xa0;kHz (purple with black outline) and 30&#xa0;kHz (green) segments, with other segments removed. Green (30&#xa0;kHz) segments with a dark outline are overprinted 200&#xa0;kHz very-high segments. Top of fourth column <bold>(D,H)</bold> show segments with mean negative (cream segments) and positive (navy segments) skew of the 30&#xa0;kHz mosaic, the most important variable in the North Calypso HVF best performing model. Bottom of fourth column <bold>(L,P)</bold> show the vector ruggedness measure (rugosity) bathymetric derivative layer, overlain by seeps, the most important variable in the South Calypso HVF best performing&#x20;model.</p>
</caption>
<graphic xlink:href="feart-09-785396-g006.tif"/>
</fig>
<p>As SP increased, segments that delineate very-high backscatter areas remained mostly stable while segments that delineate homogenous areas of seafloor increased in size (<xref ref-type="fig" rid="F6">Figure&#x20;6</xref>). Segments that delineate scarps also remain stable at higher SP levels (<xref ref-type="fig" rid="F6">Figures 6E,F,I,J</xref>). Though the ESP2 tool provides a repeatable and objective method for selecting SPs, we found the chosen SPs did not adequately identify boundaries of the hardground areas. Smaller SPs (SP9, SP14, and SP26), resulted in over segmentation, where too many segments were created without delineating conspicuous natural boundaries of features. These SPs resulted in highly granular segments, demarcating contiguous very-high backscatter areas into small segments.</p>
</sec>
<sec id="s3-5">
<title>Prediction Model Results for Seep Presence</title>
<p>Due to the imbalance of examples between &#x201c;active&#x201d; and &#x201c;inactive&#x201d; areas (<xref ref-type="sec" rid="s3-8">Section 3.8</xref>) standard model accuracy levels give a poor representation of &#x201c;goodness&#x201d; as the models correctly predict &#x201c;inactive&#x201d; areas far better than &#x201c;active&#x201d; areas. Of the 63 models run in this study, three were considered good, with Kappa &#x3e;0.6 (<xref ref-type="bibr" rid="B1">Altman, 1991</xref>). These &#x2018;good&#x2019; models were all North Calypso HVF (NCHVF) combined mosaic models, with SP 46, 75, and 50 (<xref ref-type="table" rid="T3">Table&#x20;3</xref>). NCHVF04 (combined mosaics, SP 46, accuracy: 0.75), with the highest Kappa value of 0.65, performed significantly better than the individual mosaic models with equivalent SP (<xref ref-type="fig" rid="F10">Figure&#x20;10</xref>). NCHVF04 had a 0.15 Kappa increase over NCHVF18 (30&#xa0;kHz, SP46, Kappa: 0.50, accuracy: 0.77) and &#x223c;0.20 over NCHVF11 (200&#xa0;kHz, SP46, Kappa: 0.45, accuracy:&#x20;0.75).</p>
<table-wrap id="T3" position="float">
<label>TABLE 3</label>
<caption>
<p>North Calypso HVF random forest results ordered by Kappa&#x20;score.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Model</th>
<th align="center">Mosaic</th>
<th align="center">SP</th>
<th align="center">Sensitivity</th>
<th align="center">Specificity</th>
<th align="center">PPV</th>
<th align="center">NPV</th>
<th align="center">Kappa</th>
<th align="center">Goodness</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">NCHVF04</td>
<td align="left">combined</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.67</td>
<td align="char" char=".">0.94</td>
<td align="char" char=".">0.64</td>
<td align="left">good</td>
</tr>
<tr>
<td align="left">NCHVF06</td>
<td align="left">combined</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.89</td>
<td align="char" char=".">0.62</td>
<td align="left">good</td>
</tr>
<tr>
<td align="left">NCHVF05</td>
<td align="left">combined</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.62</td>
<td align="char" char=".">0.95</td>
<td align="char" char=".">0.61</td>
<td align="left">good</td>
</tr>
<tr>
<td align="left">NCHVF20</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.71</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.55</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF21</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.75</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.52</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF18</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.76</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.58</td>
<td align="char" char=".">0.89</td>
<td align="char" char=".">0.49</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF19</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.59</td>
<td align="char" char=".">0.88</td>
<td align="char" char=".">0.49</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF12</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.55</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.49</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF03</td>
<td align="left">combined</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.45</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.46</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF13</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.71</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.58</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.46</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF11</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.72</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.51</td>
<td align="char" char=".">0.90</td>
<td align="char" char=".">0.45</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF17</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.74</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.44</td>
<td align="char" char=".">0.94</td>
<td align="char" char=".">0.44</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF14</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.69</td>
<td align="char" char=".">0.59</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.43</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF07</td>
<td align="left">combined</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.62</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.73</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">NCHVF10</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.38</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.40</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF09</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.31</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.37</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF16</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.81</td>
<td align="char" char=".">0.32</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.36</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF02</td>
<td align="left">combined</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.81</td>
<td align="char" char=".">0.27</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.31</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF08</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.24</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.29</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF01</td>
<td align="left">combined</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.24</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.29</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">NCHVF15</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.22</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.27</td>
<td align="left">fair</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>The second-best performing model, NCHVF06 (combined mosaic, SP75, accuracy: 0.82) had a lower Kappa (0.62) though higher PPV (0.73) than the best performing model, being better at predicting seep segments. NCHVF05, the third &#x201c;good&#x201d; model, had highest overall accuracy (0.84) but lower Kappa (0.61) and lower PPV (0.62). The NCHVF12 model (30&#xa0;kHz mosaic, SP100, Kappa: 0.52) had the highest PPV of 0.73 and NPV of 0.79. Of the SP levels selected by the ESP tool, only SP46 revealed a good model relationship.</p>
<p>The combined mosaic model with SP 75 (<xref ref-type="table" rid="T4">Table&#x20;4</xref>) was the best performing model for the South Calypso HVF (SCHVF) (SCHVF06; Kappa: 0.57, &#x201c;moderate,&#x201d; accuracy: 0.81); this was also the model with highest PPV (0.61) (<xref ref-type="fig" rid="F10">Figure&#x20;10</xref>). We found no significant difference between the combined model or the individual mosaic models with equivalent SP: SCHVF20 (30&#xa0;kHz, SP75, Kappa: 0.56, accuracy: 0.85) and SCHVF13 (200&#xa0;kHz, SP75, Kappa: 0.55, accuracy: 0.85), the second and third best performing models, respectively, for the South Calypso HVF. Of the SP levels selected by the ESP tool, none revealed a good model relationship.</p>
<table-wrap id="T4" position="float">
<label>TABLE 4</label>
<caption>
<p>South Calypso HVF random forest results ordered by Kappa&#x20;score.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Model</th>
<th align="center">Mosaic</th>
<th align="center">SP</th>
<th align="center">Sensitivity</th>
<th align="center">Specificity</th>
<th align="center">PPV</th>
<th align="center">NPV</th>
<th align="center">Kappa</th>
<th align="center">Goodness</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">SCHVF06</td>
<td align="left">combined</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.61</td>
<td align="char" char=".">0.92</td>
<td align="char" char=".">0.57</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF20</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.53</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.56</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF13</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.88</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.53</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.55</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF18</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.90</td>
<td align="char" char=".">0.45</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.50</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF07</td>
<td align="left">combined</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.87</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.50</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.50</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF12</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.46</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF04</td>
<td align="left">combined</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.39</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.43</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF03</td>
<td align="left">combined</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.87</td>
<td align="char" char=".">0.35</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.43</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF21</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.76</td>
<td align="char" char=".">0.75</td>
<td align="char" char=".">0.48</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF17</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.35</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF05</td>
<td align="left">combined</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.36</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">SCHVF10</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.81</td>
<td align="char" char=".">0.34</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.40</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF19</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.59</td>
<td align="char" char=".">0.88</td>
<td align="char" char=".">0.39</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF14</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.72</td>
<td align="char" char=".">0.72</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.34</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF02</td>
<td align="left">combined</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.25</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.32</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF11</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.27</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.31</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF09</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.91</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.21</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.29</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF16</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.87</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.20</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.27</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF08</td>
<td align="left">200&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.16</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.23</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF15</td>
<td align="left">30&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.16</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.23</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">SCHVF01</td>
<td align="left">combined</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.16</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.22</td>
<td align="left">fair</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>The CHVF20 30&#xa0;kHz mosaic model with SP 75 (<xref ref-type="table" rid="T5">Table&#x20;5</xref>) was the best performing model for the full Calypso HVF, incorporating all data including artefacts (Kappa: 0.51, &#x201c;moderate,&#x201d; accuracy: 0.86). The best performing model for predicting seeps, with a PPV of 0.54, was CHVF07 (combined mosaics, SP100, Kappa: 0.50, accuracy: 0.79), the second-best performing model for the full Calypso HVF. All the SP levels selected by the ESP tool (SP14, SP26, and SP46) were ranked as &#x2018;fair&#x2019; models.</p>
<table-wrap id="T5" position="float">
<label>TABLE 5</label>
<caption>
<p>Full Calypso HVF random forest results ordered by Kappa&#x20;score.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Model</th>
<th align="center">Mosaic</th>
<th align="center">SP</th>
<th align="center">Sensitivity</th>
<th align="center">Specificity</th>
<th align="center">PPV</th>
<th align="center">NPV</th>
<th align="center">Kappa</th>
<th align="center">Goodness</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="left">CHVF20</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.46</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.51</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF07</td>
<td align="center">combined</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.54</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.50</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF06</td>
<td align="center">combined</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.48</td>
<td align="char" char=".">0.94</td>
<td align="char" char=".">0.47</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF14</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.75</td>
<td align="char" char=".">0.46</td>
<td align="char" char=".">0.95</td>
<td align="char" char=".">0.45</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF13</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">75</td>
<td align="char" char=".">0.76</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.48</td>
<td align="char" char=".">0.93</td>
<td align="char" char=".">0.45</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF11</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.42</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.44</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF05</td>
<td align="center">combined</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.44</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF18</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.86</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.40</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.44</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF19</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.95</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF04</td>
<td align="center">combined</td>
<td align="char" char=".">46</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.40</td>
<td align="char" char=".">0.96</td>
<td align="char" char=".">0.42</td>
<td align="left">moderate</td>
</tr>
<tr>
<td align="left">CHVF12</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">50</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.77</td>
<td align="char" char=".">0.39</td>
<td align="char" char=".">0.95</td>
<td align="char" char=".">0.39</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF17</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.29</td>
<td align="char" char=".">0.98</td>
<td align="char" char=".">0.35</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF21</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">100</td>
<td align="char" char=".">0.70</td>
<td align="char" char=".">0.72</td>
<td align="char" char=".">0.41</td>
<td align="char" char=".">0.90</td>
<td align="char" char=".">0.34</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF10</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.81</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.29</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.33</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF03</td>
<td align="center">combined</td>
<td align="char" char=".">26</td>
<td align="char" char=".">0.78</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.29</td>
<td align="char" char=".">0.97</td>
<td align="char" char=".">0.33</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF16</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.19</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.24</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF09</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.187</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.24</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF02</td>
<td align="center">combined</td>
<td align="char" char=".">14</td>
<td align="char" char=".">0.84</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.19</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.24</td>
<td align="left">fair</td>
</tr>
<tr>
<td align="left">CHVF01</td>
<td align="center">combined</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.82</td>
<td align="char" char=".">0.80</td>
<td align="char" char=".">0.14</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.19</td>
<td align="left">poor</td>
</tr>
<tr>
<td align="left">CHVF08</td>
<td align="center">200&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.83</td>
<td align="char" char=".">0.79</td>
<td align="char" char=".">0.13</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.18</td>
<td align="left">poor</td>
</tr>
<tr>
<td align="left">CHVF15</td>
<td align="center">30&#xa0;kHz</td>
<td align="char" char=".">9</td>
<td align="char" char=".">0.85</td>
<td align="char" char=".">0.76</td>
<td align="char" char=".">0.13</td>
<td align="char" char=".">0.99</td>
<td align="char" char=".">0.17</td>
<td align="left">poor</td>
</tr>
</tbody>
</table>
</table-wrap>
</sec>
<sec id="s3-6">
<title>Hardgrounds and Correctly Predicted Seep Presence</title>
<p>By applying a Jenks Natural Breaks classification to the updated distribution of mean backscatter values for each mosaic (moving from a pixel-based distribution to a segment-based distribution) we determined where high or very-high backscatter areas (&#x201c;hardgrounds&#x201d;) are in the Calypso HVF and how they may correlate with water column acoustic flares (&#x201c;active fluid expulsion&#x201d;).</p>
<p>For the best performing model, NCHVF04 (North Calypso HVF, combined mosaics, SP46), only 1.5% (0.51&#xa0;km<sup>2</sup>) of the total area has very-high backscatter values in the 200&#xa0;kHz mosaic (&#x2212;14.0 to &#x2212;8.8&#xa0;dB) while 5.8% (1.94&#xa0;km<sup>2</sup>) of the total area has very-high backscatter values in the 30&#xa0;kHz mosaic (&#x2212;2.4 to &#x2212;7.2&#xa0;dB) (<xref ref-type="fig" rid="F10">Figure&#x20;10C</xref>).</p>
<p>Of the very-high 200&#xa0;kHz backscatter segments, 0.3&#xa0;km<sup>2</sup> (0.87% of the total North Calypso HVF area) were also true positives (i.e. correctly predicted active fluid expulsion segments) while 0.2&#xa0;km<sup>2</sup> (0.67% of the total North Calypso HVF area) were false positives (i.e. incorrectly predicted active fluid expulsion) segments. Of the very-high 30&#xa0;kHz backscatter segments, only 0.07&#xa0;km<sup>2</sup> were true positives; 1.37&#xa0;km<sup>2</sup> were false positives (<xref ref-type="fig" rid="F10">Figure&#x20;10C</xref>). The remainder comprised true negatives and false negatives.</p>
</sec>
<sec id="s3-7">
<title>Predictor Variable Importance</title>
<p>The nine most important predictor variables for the best performing model, NCHVF04 (combined 30 and 200&#xa0;kHz, SP46), include a combination of backscatter and bathymetric derived variables (<xref ref-type="fig" rid="F7">Figure&#x20;7A</xref>). Skewness of 30&#xa0;kHz, the distribution asymmetry of backscatter values from the pixels that construct a segment, has the highest relative importance to all other variables, meaning this variable would have the most negative impact on the model accuracy if removed. Segments with positive skew (segments that were made up of a majority of low mean dB values with a few very-high values in the tail, i.e. areas of soft sediment with flares) were aligned with areas that contained known seeps, (<xref ref-type="fig" rid="F6">Figures 6D,H</xref>). Ruggedness and slope were the next most important predictor variables for NCHVF04. Mean EM2040 backscatter values (i.e. mean 200&#xa0;kHz values that contribute to each segment) was the fifth most important variable. For the second-best performing model, NCHVF06, skewness of 30&#xa0;kHz was the most important variable while it was third most important for the third best model NCHVF05.</p>
<fig id="F7" position="float">
<label>FIGURE 7</label>
<caption>
<p>Variable importance plots of the &#x201c;mean decrease in accuracy&#x201d; measure. <bold>(A)</bold> North Calypso HVF best performing model NCHVF04: combined mosaics, SP46; <bold>(B)</bold> South Calypso HVF best performing model SCHVF06: combined mosaics, SP75.</p>
</caption>
<graphic xlink:href="feart-09-785396-g007.tif"/>
</fig>
<p>The seven most important predictor variables for model SCHVF06 (combined 30 and 200&#xa0;kHz, SP75) were a combination of backscatter and bathymetric derived variables, with vector ruggedness measure the most important (<xref ref-type="fig" rid="F6">Figures 6L,P</xref>, <xref ref-type="fig" rid="F7">7B</xref>). Ruggedness has the highest relative importance to all other variables, i.e. if removed, this variable would reduce the model accuracy the most. Fine bathymetric position index was the next most important variable. Skewness of the 30&#xa0;kHz was 13th in relative importance for this&#x20;model.</p>
</sec>
<sec id="s3-8">
<title>Seafloor Observations</title>
<p>Towed camera footage, though biased toward discrete seafloor areas of interest, provides additional information about active seafloor fluid expulsion that accords with model results. Though not spatially extensive, video footage confirms the assumption that very-high backscatter classes are due to indurated or lithified seafloor. Carbonate platforms and lithified seafloor observed in the towed camera aligned with high and very-high backscatter areas observed in the mosaics (<xref ref-type="fig" rid="F8">Figure&#x20;8</xref>). This relationship was more obvious however in the 30&#xa0;kHz mosaic and for FOI-02 and -03 (<xref ref-type="fig" rid="F8">Figures 8D,E</xref>, <xref ref-type="fig" rid="F9">9</xref>). No shell hash, mussel beds, or other potential reflectors traditionally also associated with fluid expulsion zones, and potential causes of high seafloor backscatter, where observed in the video footage. FOI-01 in the North Calypso HVF (<xref ref-type="fig" rid="F8">Figures 8A,B</xref>) exhibits a high intensity of fluid expulsion coinciding with bioturbated sediment (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.1-3). Videos show shimmery fluid (due to density differences) escaping from the perimeters of anhydrite mounds (&#x223c;1&#x20;m diameter) covered in white filamentous bacteria (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.4-6); less intensive bubble expulsion was also observed. This fluid expulsion coincided with very-high backscatter in the 200&#xa0;kHz mosaic (<xref ref-type="fig" rid="F8">Figure&#x20;8A</xref>) and broadly low backscatter with smaller patches of very-high backscatter in the 30&#xa0;kHz mosaic (<xref ref-type="fig" rid="F8">Figure&#x20;8B</xref>).</p>
<fig id="F8" position="float">
<label>FIGURE 8</label>
<caption>
<p>Towed camera transects (black lines) for three features of interest (FOI; see <xref ref-type="fig" rid="F1">Figure&#x20;1B</xref> for locations) mapped over 200&#xa0;kHz mosaic <bold>(A,C,E)</bold> and 30&#xa0;kHz mosaic <bold>(B,D,E)</bold> show a variety of substrates both with and without active fluid expulsion. Black dots - seep base locations; mosaic resolution 2&#xa0;m; projection NZTM; white lines: segments of best performing models for North (SP46 combined) and South (SP75 combined). Numbers correspond to still images in <xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.</p>
</caption>
<graphic xlink:href="feart-09-785396-g008.tif"/>
</fig>
<fig id="F9" position="float">
<label>FIGURE 9</label>
<caption>
<p>Still images for three features of interest (FOI; see <xref ref-type="fig" rid="F1">Figure&#x20;1B</xref> for FOI locations). Numbers correspond to approximate locations along towed camera transects in <xref ref-type="fig" rid="F8">Figure&#x20;8</xref>. White dashed lines highlight where fluid was observed exiting the seafloor; white circles highlight observable bubbles.</p>
</caption>
<graphic xlink:href="feart-09-785396-g009.tif"/>
</fig>
<p>FOI-02 in the South Calypso HVF (<xref ref-type="fig" rid="F8">Figures 8C,D</xref>) hosted a wide variety of substrates: sedimented regions with high-flux fluid expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.7); bioturbated sediment (as evidenced by active burrowing within surface sediment) with gas expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.8); hardground regions with low-flux fluid expulsion from the perimeters of, or in between, broken platforms (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.9) or rocky piles of hardgrounds (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.10); occasional anemone gardens (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.9) and mussel beds (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.10); areas of extensive white filamentous bacterial mats with very-high-flux gas and liquid expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.11). Of note were the many instances of liquid and gas bubble expulsion at the same area of seabed, with one high flux gas and fluid expulsion area of rocky substrate blanketed by thick white bacterial mats (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.12). These hardground regions coincided with very-high backscatter intensity in both mosaics. Other very-high backscatter intensity patches in the South Calypso HVF survey area were not sampled with the towed camera.</p>
<p>FOI-03 in the North Calypso HVF (<xref ref-type="fig" rid="F8">Figures 8E,F</xref>), hosts fluid expulsion coinciding with a broad break of slope (<xref ref-type="fig" rid="F1">Figure&#x20;1B</xref>). This seafloor was characterized by: bioturbated sedimented regions with no fluid expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.13); white and lilac colored filamentous bacterial with and without gas bubble expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.14), with lilac colored bacteria corresponding with areas of minimal fluid expulsion (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.15); &#x223c;0.5&#xa0;m diameter anhydrite mounds with high flux liquid expulsion (observed in towed camera footage as shimmery film) (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.16); hardground regions with lower flux gas bubble expulsion, from the perimeters of, or in between, broken platforms (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.17) and rocky piles of hardgrounds; and occasional anemone gardens (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>.18). Here, hardground areas corresponded to very-high backscatter intensity in the 30&#xa0;kHz mosaic and also in the 200&#xa0;kHz mosaic, though the overall very-high intensity signal of the 200&#xa0;kHz mosaic obscured direct comparisons between very-high 200&#xa0;kHz values and seafloor observations. More extensive and very-high backscatter intensity patches in the North Calypso HVF survey area were not sampled with the towed camera on this survey.</p>
</sec>
</sec>
<sec sec-type="discussion" id="s4">
<title>Discussion</title>
<sec id="s4-1">
<title>Quantitative Links Between Active Seepage and Seafloor Characteristics</title>
<p>We were able to link water-column acoustic flares (as an active fluid expulsion proxy) to specific backscatter and bathymetric derivatives of the seafloor using RF models at a shallow hydrothermal vent field. The best performing model (North Calypso HVF, combined mosaics, SP46, with overall accuracy: 0.75; PPV: 0.67; Kappa accuracy: 0.65) demonstrated the potential of using GEOBIA analysis, image segmentation, and RF modelling to predict regions of potential fluid expulsion based on seafloor characteristics (<xref ref-type="fig" rid="F10">Figure&#x20;10</xref>). Although the South Calypso models were not as accurate, they still performed well in highlighting areas where active fluid expulsion were observed (<xref ref-type="fig" rid="F10">Figure&#x20;10</xref>).</p>
<fig id="F10" position="float">
<label>FIGURE 10</label>
<caption>
<p>Final model predictions for the best performing models of <bold>(A)</bold> North Calypso HVF (SP46) and <bold>(B)</bold> South Calypso HVF (SP75) with seep locations (black dots) overlain, alongside <bold>(C)</bold> segment area and very-high backscatter area confusion matrix results for the best performing NCHVF&#x20;model.</p>
</caption>
<graphic xlink:href="feart-09-785396-g010.tif"/>
</fig>
<p>We were unable however to link gas flares specifically to &#x201c;hardgrounds,&#x201d; or very-high backscatter areas. Although areas of very-high backscatter areas in the 200&#xa0;kHz mosaic were associated with predicted seep locations in the North, very-high backscatter dominated the whole mosaic most likely due to sand-dominant seafloor surface substrate overprinting discrete hardground areas, including areas that lacked seeps (<xref ref-type="fig" rid="F8">Figure&#x20;8A</xref>). This finding contradicted what was observed in the 30&#xa0;kHz mosaic, with seeps associated with a range of backscatter values, from low to very-high. These results however, combined with those provided by the RF variable importance measure, provide us with initial seafloor indicators associated with active fluid expulsion.</p>
<p>
<xref ref-type="bibr" rid="B61">Naudts et&#x20;al. (2008)</xref> observed that while fluid expulsion activity from shallow (&#x3c;200&#xa0;m water depth) seeps in the NW Black Sea and &#x201c;hardground&#x201d; backscatter were often correlated, higher fluid expulsion occurred adjacent to, or nearby, these hardgrounds. Along the Hikurangi Margin <xref ref-type="bibr" rid="B62">Naudts et&#x20;al. (2010)</xref> noted that fluid expulsion from seeps in water deeper than 500&#xa0;m escaped from sandy sediments away from highly reflective backscatter hardground platforms. <xref ref-type="bibr" rid="B56">Mitchell et&#x20;al. (2018)</xref> however, supported the intuitive link between gas flares and potential hardgrounds when superimposing water column acoustic flares over anomalous seafloor backscatter intensities from &#x223c;1,000 to 2000&#xa0;m in the Gulf of Mexico. <xref ref-type="bibr" rid="B76">Thorsnes et&#x20;al. (2019)</xref> showed on the Norwegian continental shelf (&#x3c;400&#xa0;m) that spatial relationships between hardgrounds and seeps are not always consistent, particularly when considering relict or dormant systems, or for fluid expulsion systems occurring from seafloor with more highly variable substrate&#x20;types.</p>
</sec>
<sec id="s4-2">
<title>Implications for Predictive Modelling of Active Fluid Expulsion</title>
<p>Whilst we demonstrate from this example that areas of very-high seafloor backscatter are not good predictors of gas flares in this context, we suggest finer-scale textural indicators may be a useful tool to determine locations of active seeps. Skewness of the 30&#xa0;kHz mosaic segments, whereby segments with positive skew were associated with known seeps, was the most influential variable in the best performing model and ranked highly in most of the other models. Positive skew describes the long-tailed distribution of all the 30&#xa0;kHz pixels that contribute to each segment, with a higher density of relatively lower pixel values with few much higher values (the positive tail). This corresponds to textures observable in the 30&#xa0;kHz mosaic, for e.g. <xref ref-type="fig" rid="F8">Figure&#x20;8B</xref>, where discrete patches of high to very-high backscatter values are scattered among surrounding lower backscatter values.</p>
<p>Towed camera footage shows a similar relationship&#x2014;areas of rubble or broken hardgrounds with minimal to no fluid flux were interspersed with unconsolidated sediment that hosted the most ebullient seeps (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>). Although we found smaller SPs did not contribute to any of the best performing models, segment skewness may more aptly capture the fine-scale seafloor textures that are lost when using segmentation and GEOBIA methods, while still allowing for noise inherent in seafloor backscatter mosaics to be accounted&#x20;for.</p>
</sec>
<sec id="s4-3">
<title>A Combined Frequency Approach to Improve Model Accuracy</title>
<p>Results from the Calypso HVF demonstrate that combining backscatter mosaics from multiple frequencies increased model accuracy compared to using individual mosaics. The combined models were the best performers for both the North and South Calypso HVFs. We suggest the improved predictive power, compared to a single frequency, is achieved by capturing information from both surface backscatter and notable acoustic returns in the immediate sub seafloor surface. <xref ref-type="bibr" rid="B10">Buscombe and Grams (2018)</xref> suggested that increasing dimensionality in a seafloor classification, by using multiple frequency mosaics, provides more points of understanding for a model to draw upon when determining substrate boundaries. Classification models using the single 30&#xa0;kHz mosaics for North and South also outperformed their equivalent 200&#xa0;kHz-based models at each of the segmentation scales, both in overall model accuracy and predictive power. In the North in particular, the 30&#xa0;kHz mosaic better highlights areas of very-high to high backscatter than the 200&#xa0;kHz, as there is difficulty differentiating between sandy substrate and hardgrounds in the higher frequency mosaic using backscatter alone (<xref ref-type="fig" rid="F4">Figure&#x20;4C</xref>). Sand grains (&#x3c;4&#x20;<italic>&#x3d5;</italic>), as observed in the sediment samples collected in the North Calypso HVF, are strong acoustic scatterers, comparable to hard substrate (<xref ref-type="bibr" rid="B52">Lurton and Lamarche, 2015</xref>). The lower frequency sounder was able to penetrate beneath the sandy overprinting veneer and better delineate the hardgrounds beneath. Work has been undertaken to estimate signal penetration depths, with low frequency systems having the greatest penetration potential: e.g. 4&#xa0;m (<xref ref-type="bibr" rid="B27">Hillman et&#x20;al., 2017</xref>) or 12&#xa0;m (<xref ref-type="bibr" rid="B72">Schneider Von Deimling et&#x20;al., 2013</xref>) penetration for a 12&#xa0;kHz signal; up to 7&#xa0;m penetration for 27&#x2013;30&#xa0;kHz (<xref ref-type="bibr" rid="B58">Mitchell, 1993</xref>), and decimeter penetration for a 95&#xa0;kHz signal (<xref ref-type="bibr" rid="B20">Fonseca et&#x20;al., 2002</xref>). This demonstrates the usefulness of employing a lower frequency sounder than would typically be considered ideal for these depth ranges (&#x3c;200&#xa0;m) to reveal sub-surface structures.</p>
<p>The usefulness of using a lower frequency sounder, or combining two frequency sounders, for classifying the seafloor and interpreting sediment at depth has been demonstrated elsewhere. <xref ref-type="bibr" rid="B30">Hughes Clarke (2015)</xref> initially demonstrated that so long as a minimum separation of one octave between frequencies is maintained, two discrete MBES frequencies may enable frequency differences to be exploited and allow for improved discrimination between surface seafloor characteristics and subsurface information. <xref ref-type="bibr" rid="B21">Gaida et&#x20;al. (2018)</xref> found that using a 100 and 400&#xa0;kHz combination increased substrate discrimination in the Bedford Basin, Canada, compared with using a single frequency. Working on the same dataset, <xref ref-type="bibr" rid="B9">Brown et&#x20;al. (2019)</xref>, observed coarse sub-surface dredge spoils in a 100&#xa0;kHz mosaic that were not observed in the 400&#xa0;kHz mosaic. <xref ref-type="bibr" rid="B17">Feldens et&#x20;al. (2018)</xref> found that lower frequency data were more sensitive to substrate changes, highlighting buried seafloor structures that were not detected in higher frequency data and highlighted the greatest contrast between seafloor facies. This allowed for additional sediment classes to be produced by combining surface acoustic information with subsurface acoustic information observed in the 100&#xa0;kHz frequency backscatter data. <xref ref-type="bibr" rid="B10">Buscombe and Grams (2018)</xref> showed that multi-spectral data and spatially-aware machine learning algorithms that do not rely on assumptions of independence in substrate models may better determine substrate classifications. <xref ref-type="bibr" rid="B32">Janowski et&#x20;al. (2018)</xref> however, highlighted that multi-spectral seafloor mosaics, though ideal, are not always required for seafloor discrimination. They showed a frequency pair (150 and 400&#xa0;kHz) can provide enough additional frequency information to improve overall accuracy of RF models. Specific to shallow hydrothermal vent fields, penetrative backscatter differences may be useful in detecting emergent seeps or sub-surface sediment modification or classes that are difficult to detect from a conventional single frequency mapping approach, while spatially-aware machine learning algorithms may allow for the incorporation of broader geological influences such as fault scarps.</p>
</sec>
<sec id="s4-4">
<title>Factors Affecting Model Accuracy</title>
<p>We propose differences in model accuracy between the North and South Calypso HVF are the result of two different seep settings, both in surrounding substrate and seep style, and may imply two distinct or diverging seep systems. We also speculate that a complex arrangement of precipitated minerals, including hydrothermal sulfates (e.g. anhydrites) as well as carbonates, may be producing the very-high seafloor backscatter anomalies at the seafloor surface of the two sites. Overprinting by terrigenous and volcanic sediments may also affect model accuracy due to hardground burial however sedimentation rates in the Bay of Plenty, in particular the Calypso site are relatively low (in the order of decimeters per 10,000&#xa0;years) (<xref ref-type="bibr" rid="B37">Kohn and Glasby, 1978</xref>; <xref ref-type="bibr" rid="B5">Bostock et&#x20;al., 2018</xref>).</p>
<p>The South Calypso HVF seeps are represented by the very-high backscatter mounds surrounded by medium silt seafloor (predominantly low backscatter in the 200&#xa0;kHz and medium in the 30&#xa0;kHz mosaic). The North Calypso HVF in contrast exhibits a constellation of seeps over varying substrate types. The South Calypso HVF is nearer to the Whakat&#x101;ne Graben axis than the North and noticeably, the South Calypso HVF seep clusters exhibit more ebullient liquid and gas flux in video footage than what we observed in the North. This nearness of the South Calypso HVF to the graben axis, its more active appearance, and more discrete emplacement style lacking extensive hardgrounds may imply a younger hydrothermal seep than the North. In addition, a higher abundance of gas at depth in the South Calypso HVF sediments may contribute to the higher intensity response observed in the 30&#xa0;kHz mosaic. The relatively lower gas flux, compared to the South, and extensive sediment settling on most of the hardground platforms observed in the towed camera suggest the North hardground areas are less active and have been emplaced over a longer period of time (thousands of years) (<xref ref-type="bibr" rid="B7">Bowden et&#x20;al., 2013</xref>). The combined extensive hardgrounds and active seeps in the North suggest a combined seep system of active hydrothermal venting and longer-lived carbonate hardgrounds. Largely, the emplaced hardgrounds closely resemble authigenic carbonate platforms observed at cold methane seeps, implying a gentler hydrothermal gradient with the potential to encourage microbial anaerobic oxidation of methane to&#x20;occur.</p>
<p>
<xref ref-type="bibr" rid="B66">Procesi et&#x20;al. (2019)</xref> pose that the Calypso HVF is a sediment-hosted geothermal system (SHGS). SHGSs are geological vent and seep systems that straddle both strictly hydrothermal and hydrocarbon arrangements; they emit both mantle derived CO<sub>2</sub> and biotic CH<sub>4</sub>. For Calypso HVF, <xref ref-type="bibr" rid="B75">Stoffers et&#x20;al. (1999)</xref> and <xref ref-type="bibr" rid="B36">Kamenev et&#x20;al. (1993)</xref> both reported the presence of hydrocarbons in vent fluid and bottom waters. <xref ref-type="bibr" rid="B6">Botz et&#x20;al. (2002)</xref> measured 83.61% CO<sub>2</sub> and 6.196% CH<sub>4</sub> within the North Calypso HVF vent gas, with a temperature of 201&#xb0;C. Values of 72.10% CO<sub>2</sub> and 9.898% CH<sub>4</sub> in vent gas and 51.79% CO<sub>2</sub> and 1.182% CH<sub>4</sub> in vent water, with a temperature of 181&#xb0;C recorded at the South Calypso HVF. While the carbon isotope values observed between &#x2212;3.4&#x2030; and &#x2212;5.5&#x2030; of the reference standard for &#x3b4;<sup>13</sup>C (Pee Dee Belemnite (PDB)) indicate a shallow magmatic source for the CO<sub>2</sub> gas, &#x3b4;<sup>13</sup>C isotope values between &#x2212;24.6&#x2030; and -28.9&#x2030; PDB at Calypso HVF also indicate CH<sub>4</sub> sourced from thermal maturation of organic matter (thermogenesis) within marine sediments (<xref ref-type="bibr" rid="B6">Botz et&#x20;al., 2002</xref>).</p>
<p>
<xref ref-type="bibr" rid="B71">Sarano et&#x20;al. (1989)</xref> and <xref ref-type="bibr" rid="B36">Kamenev et&#x20;al. (1993)</xref> reported anhydrite mounds up to 8&#xa0;m in height. Numerous smaller (&#x223c;1&#x2013;2&#xa0;m high) anhydrite mounds, with observable liquid escape (shimmery water-column features in the video footage) and associated filamentous bacteria, were observed during the 2018 voyage. No mounds were detected on the same scale as the previously observed mound (<xref ref-type="fig" rid="F9">Figure&#x20;9</xref>). The small anhydrite mounds observed during the 2018 voyage may be a precursor to larger cones, if the vent pathway is maintained, or eroded versions of formerly larger features. <xref ref-type="bibr" rid="B28">Hocking et&#x20;al. (2010)</xref> observed that the largest mound had visibly eroded over two&#xa0;decades, suggesting high temperature fluid expulsion may have waned or relocated in the North Calypso HVF since original observations were made by <xref ref-type="bibr" rid="B71">Sarano et&#x20;al. (1989)</xref>. Anhydrite forms as hot calcium-rich hydrothermal fluids mix with cold sulfate-rich sea water (<xref ref-type="bibr" rid="B71">Sarano et&#x20;al., 1989</xref>; <xref ref-type="bibr" rid="B25">Hannington et&#x20;al., 2001</xref>; <xref ref-type="bibr" rid="B48">Lowell et&#x20;al., 2003</xref>). The retrograde solubility for anhydrite is &#x3e; 150&#xb0;C; below this threshold, anhydrite dissolves (<xref ref-type="bibr" rid="B60">Mottl, 1983</xref>). Without a constant supply of hot vent fluid, anhydrite dissolves at shallow depths (&#x3c;200&#xa0;m) (<xref ref-type="bibr" rid="B47">Lowell and Yao, 2002</xref>; <xref ref-type="bibr" rid="B48">Lowell et&#x20;al., 2003</xref>). Hardgrounds however exist on timescales many orders of magnitude longer than active fluid expulsion (<xref ref-type="bibr" rid="B3">Bayon et&#x20;al., 2009</xref>; <xref ref-type="bibr" rid="B45">Liebetrau et&#x20;al., 2010</xref>; <xref ref-type="bibr" rid="B7">Bowden et&#x20;al., 2013</xref>); coexistent hardgrounds and anhydrite mounds suggest both long-lived and contemporary fluid expulsion. Consequently, modelling flares to hardgrounds may not capture such timescale differences.</p>
</sec>
<sec id="s4-5">
<title>Improvements and Future Applications</title>
<p>Fine tuning our approach may lead to more sophisticated means of locating and differentiating between active and relict fluid expulsion areas. Additional recommendations for future mapping might consider the following elements in each step of the modeling pathway.</p>
</sec>
<sec id="s4-6">
<title>Acquisition</title>
<p>A stratified random sampling regime, combined with more extensive video footage to observe first order indications of active and inactive fluid expulsion, would allow more robust associations between gas flares/fluid expulsion and seafloor characteristics. This would allow the unique seafloor textural differences associated with both to be captured and fully modeled. A single-pass survey using an integrated multi-spectral system for multi-frequency seafloor analysis (<xref ref-type="bibr" rid="B9">Brown et&#x20;al., 2019</xref>) would ensure MBES calibration, correction, and data reduction issues could be reduced by collecting varying frequency information on a ping-by-ping basis. Further, repeat surveys to capture change detection in both the seafloor and water column acoustic backscatter would allow us to integrate short time frame fluctuations in a dynamic seep system into the&#x20;model.</p>
</sec>
<sec id="s4-7">
<title>Processing</title>
<p>Seep identification using higher frequency (200&#xa0;kHz) water-column acoustic data would allow us to interrogate more forms of fluid expulsion. We identified fluid expulsion using 30&#xa0;kHz water-column acoustic data which favors gas expulsion. Additional fluid expulsion evidence may be found in the 200&#xa0;kHz water-column data, e.g. liquid expulsion that lacks a gas fraction. Image segmentation and pre-classification could also be used to remove obvious artefacts prior to segmentation.</p>
</sec>
<sec id="s4-8">
<title>Modelling</title>
<p>Sampling hardground platforms in both regions, to determine sediment mineralogy, combined with acoustic information, discrete camera sampling, and geochronology could be used to determine the genesis or timescales of the active fluid expulsion regions. Additionally, seep differences could be modeled to further understand seafloor modifications possible within of a sediment-hosted geothermal system.</p>
</sec>
</sec>
<sec sec-type="conclusion" id="s5">
<title>Conclusion</title>
<p>We demonstrate that a dual frequency approach, within the bounds of a MBES survey, leads to improved seep prediction. Combining the penetration and resolution of two distinct MBES frequencies can provide added value for the interpretation of seafloor structure from backscatter data. Understanding the potential of different acoustic frequencies when targeting specific seafloor substrates is necessary to inform decisions of vessel choice or limitations when preparing for a survey of a key substrate type. We show that extensive and precise ground-truthing (through video, grabs, and cores) that enable strong associations between paired or multi-frequency seafloor maps are required to advance our understanding of penetration depth and influence of sounder choice on seafloor substrate classification. Understanding the limits imposed by chosen frequencies and sonar systems will increase these associations, while improving integration methods for paired frequency systems.</p>
<p>The main outcome of this study is that the extensive hardgrounds of the Calypso HVF are not directly linked with gas flares within this shallow hydrothermal system, while unconsolidated sediment nearby hosts higher flux expulsion. This aligns with the development and cessation of other submarine fluid expulsion system (<xref ref-type="bibr" rid="B61">Naudts et&#x20;al., 2008</xref>; <xref ref-type="bibr" rid="B35">Judd and Hovland, 2009</xref>; <xref ref-type="bibr" rid="B62">Naudts et&#x20;al., 2010</xref>). Seafloor fluid systems will refashion themselves on decadal timescales due to vent instability, clogging from deposited minerals, modification of the sediment, or rearrangement due to ongoing extensional processes and tectonic stresses (<xref ref-type="bibr" rid="B14">Desbruy&#xe8;res, 1998</xref>; <xref ref-type="bibr" rid="B7">Bowden et&#x20;al., 2013</xref>; <xref ref-type="bibr" rid="B42">Levin et&#x20;al., 2016</xref>). Once a seep self-seals, fluid will migrate to a more permeable pathway. Being able to remotely predict active and inactive regions of fluid expulsion will prove a useful tool in rapidly identifying seeps in legacy datasets, as well as textural metrics that will aid in locating nascent, senescent, or even extinct seeps during a survey, where important communities may persist even if they appear inactive. Understanding the seafloor qualities best able to predict active or inactive fluid expulsion will allow us to establish primary controls on fluid expulsion in a shallow marine environment and further understand seep succession stages and evolution.</p>
</sec>
</body>
<back>
<sec id="s6">
<title>Data Availability Statement</title>
<p>The raw data supporting the conclusion of this article will be made available by the authors, without undue reservation.</p>
</sec>
<sec id="s7">
<title>Author Contributions</title>
<p>All authors give final approval of the article to be published. ES conceived and designed the project; processed and analyzed data; generated segmentation and random forest analysis output; substantially drafted, constructed, and revised the article and figures. GL was voyage leader of QUOI-TAN1806 voyage that produced the data and was science leader of public funded program that funded the research. GL and VL funded the voyage from project &#x201c;Building capability for <italic>in situ</italic> quantitative characterization of the ocean water-column using acoustic multibeam backscatter data&#x201d; (2017&#x2013;2018) - Royal Society of New&#x20;Zealand Catalyst Fund. JW, VL, and GL supervised the project; provided analytical support and guidance; critically reviewed and revised the article. SW acquired and processed data; provided technical and analytical support; revised the article. YL acquired and processed data; provided technical support; revised the article. EH acquired and processed data; provided technical support; revised the article. AP acquired and processed data; provided technical support; revised the article.</p>
</sec>
<sec id="s8">
<title>Funding</title>
<p>ES is funded under the Australian Research Council&#x2019;s Special Research Initiative for Antarctic Gateway Partnership (Project ID SR140300001). GL and VL were funded by The Royal Society of New&#x20;Zealand Catalyst Fund project: &#x201c;Building capability for <italic>in situ</italic> quantitative characterization of the ocean water-column using acoustic multibeam backscatter data (2017&#x2013;2018)&#x201d;. This research contributes to the Ministry of Business, Innovation, and Employment (MBIE) funded Smart Ideas Grant: &#x201c;Broadband acoustic characterization of free gases in the ocean water (Contract C01X1915)&#x201d; and is further supported by the MBIE Strategic Science Investment Fund (SSIF) Marine Geological Processes Program of the NIWA Coasts and Oceans Centre (COPR).</p>
</sec>
<sec sec-type="COI-statement" id="s9">
<title>Conflict of Interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="disclaimer" id="s10">
<title>Publisher&#x2019;s Note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
<ack>
<p>We thank the captain, crew, and science team of the RV <italic>Tangaroa</italic> QUOI/TAN1806 voyage. We also thank Yves Le Gonidec, Arnaud Gaillot (IFREMER); Tom Weber, Liz Weidner (UNH); Amy Nau (UTAS); Lisa Northcote (NIWA).</p>
</ack>
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