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<front>
<journal-meta>
<journal-id journal-id-type="publisher-id">Front. Earth Sci.</journal-id>
<journal-title>Frontiers in Earth Science</journal-title>
<abbrev-journal-title abbrev-type="pubmed">Front. Earth Sci.</abbrev-journal-title>
<issn pub-type="epub">2296-6463</issn>
<publisher>
<publisher-name>Frontiers Media S.A.</publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id pub-id-type="publisher-id">1024037</article-id>
<article-id pub-id-type="doi">10.3389/feart.2022.1024037</article-id>
<article-categories>
<subj-group subj-group-type="heading">
<subject>Earth Science</subject>
<subj-group>
<subject>Original Research</subject>
</subj-group>
</subj-group>
</article-categories>
<title-group>
<article-title>Quantitative assessment of the impacts of climate and human activities on streamflow of the Lancang-Mekong river over the recent decades</article-title>
<alt-title alt-title-type="left-running-head">Li et al.</alt-title>
<alt-title alt-title-type="right-running-head">
<ext-link ext-link-type="uri" xlink:href="https://doi.org/10.3389/feart.2022.1024037">10.3389/feart.2022.1024037</ext-link>
</alt-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname>Li</surname>
<given-names>Renzhi</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1968503/overview"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Huang</surname>
<given-names>He Qing</given-names>
</name>
<xref ref-type="aff" rid="aff3">
<sup>3</sup>
</xref>
<xref ref-type="aff" rid="aff4">
<sup>4</sup>
</xref>
<uri xlink:href="https://loop.frontiersin.org/people/1621855/overview"/>
</contrib>
<contrib contrib-type="author" corresp="yes">
<name>
<surname>Wang</surname>
<given-names>Zhonggen</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2">
<sup>2</sup>
</xref>
<xref ref-type="corresp" rid="c001">&#x2a;</xref>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Zhao</surname>
<given-names>Ruxin</given-names>
</name>
<xref ref-type="aff" rid="aff1">
<sup>1</sup>
</xref>
</contrib>
</contrib-group>
<aff id="aff1">
<sup>1</sup>
<institution>National Institute of Natural Hazards</institution>, <institution>Ministry of Emergency Management of the People&#x2019;s Republic of China</institution>, <addr-line>Beijing</addr-line>, <country>China</country>
</aff>
<aff id="aff2">
<sup>2</sup>
<institution>Key Laboratory of Compound and Chained Natural Hazards Dynamics</institution>, <institution>Ministry of Emergency Management of China</institution>, <addr-line>Beijing</addr-line>, <country>China</country>
</aff>
<aff id="aff3">
<sup>3</sup>
<institution>Institute of Geographic Sciences and Natural Resources Research</institution>, <institution>Chinese Academy of Sciences</institution>, <addr-line>Beijing</addr-line>, <country>China</country>
</aff>
<aff id="aff4">
<sup>4</sup>
<institution>University of Chinese Academy of Sciences</institution>, <addr-line>Beijing</addr-line>, <country>China</country>
</aff>
<author-notes>
<fn fn-type="edited-by">
<p>
<bold>Edited by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/1474987/overview">Dabang Jiang</ext-link>, Institute of Atmospheric Physics (CAS), China</p>
</fn>
<fn fn-type="edited-by">
<p>
<bold>Reviewed by:</bold> <ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/278178/overview">Mohamad N. Azra</ext-link>, Universiti Malaysia Terengganu, Malaysia</p>
<p>
<ext-link ext-link-type="uri" xlink:href="https://loop.frontiersin.org/people/859389/overview">Haiyun Shi</ext-link>, Southern University of Science and Technology, China</p>
</fn>
<corresp id="c001">&#x2a;Correspondence: Zhonggen Wang, <email>wangzg@igsnrr.ac.cn</email>
</corresp>
<fn fn-type="other">
<p>This article was submitted to Hydrosphere, a section of the journal Frontiers in Earth Science</p>
</fn>
</author-notes>
<pub-date pub-type="epub">
<day>04</day>
<month>01</month>
<year>2023</year>
</pub-date>
<pub-date pub-type="collection">
<year>2022</year>
</pub-date>
<volume>10</volume>
<elocation-id>1024037</elocation-id>
<history>
<date date-type="received">
<day>20</day>
<month>08</month>
<year>2022</year>
</date>
<date date-type="accepted">
<day>05</day>
<month>12</month>
<year>2022</year>
</date>
</history>
<permissions>
<copyright-statement>Copyright &#xa9; 2023 Li, Huang, Wang and Zhao.</copyright-statement>
<copyright-year>2023</copyright-year>
<copyright-holder>Li, Huang, Wang and Zhao</copyright-holder>
<license xlink:href="http://creativecommons.org/licenses/by/4.0/">
<p>This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.</p>
</license>
</permissions>
<abstract>
<p>The impacts of climate and human activities exerted on streamflow over the recent decades in the Lancang-Mekong River Basin (LMRB) have been examined in separate forms and this study performed an integrated quantitative evaluation. Using the meteorological and hydrological data measured in LMRB during 1961&#x2013;2015, we analyzed the varying trend and abrupt change characteristics of streamflow along the river course, and constructed a SWAT hydrological model to quantitatively evaluate the contributions of climate and human activities by taking into account their spatial heterogeneity. At the yearly timescale, the results show that for significant complex changes in streamflow along the Lancang-Mekong River, the ratios of the contributions of the impacts of human activity (<inline-formula id="inf1">
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<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>) before 2000 to those after 2000 are under 15.2%, 17.5% and 32.4% respectively in the source area above Jiuzou (China), the upper area between Jiuzhou and Yunjinghong (China), and the middle area between Jiuzhou and Vientiane (Laos). In the lower area between Vientiane and Stung Treng (Cambodia), <inline-formula id="inf2">
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</inline-formula> was only 22.6% before 2000 and yet dramatically increased to 59.1% after 2000. While the same situation happened at the seasonal time scale, <inline-formula id="inf3">
<mml:math id="m3">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
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</inline-formula> has relatively larger <inline-formula id="inf4">
<mml:math id="m4">
<mml:mtext>values</mml:mtext>
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</inline-formula> during dry seasons than in wet seasons. In contrast to the gradually increased impacts of human activities, the impacts of climate on streamflow gradually decreased from the upper to the lower areas. Furthermore, the impacts of the changes in land use types account for about 1/3 in the Lancang River Basin and yet reaches more than 1/2 in the Mekong River Basin.</p>
</abstract>
<kwd-group>
<kwd>Lancang-Mekong river basin</kwd>
<kwd>climate change</kwd>
<kwd>human activities</kwd>
<kwd>streamflow</kwd>
<kwd>SWAT model</kwd>
</kwd-group>
</article-meta>
</front>
<body>
<sec id="s1">
<title>1 Introduction</title>
<p>Over the last several decades, streamflow regimes have experienced significant alterations around the world (<xref ref-type="bibr" rid="B43">Zhao et al., 2015</xref>). Due to the combined impacts of climate and human activities on streamflow process in large river basins, streamflow regime shifts often in complex cascading forms (Best 2019; Hughes et al., 2013). Understanding physical mechanisms by which the two drivers of changes take effect and their relative contributions on streamflow regime shift have been a hot topic for research for bolstering the management of river basin systems (<xref ref-type="bibr" rid="B14">Hu et al., 2021</xref>; <xref ref-type="bibr" rid="B2">Ali et al., 2022</xref>).</p>
<p>Changes in climate and land use types have long been regarded as the major factors influencing the water resources in the Lancang-Mekong River Basin (LMRB) (e.g., <xref ref-type="bibr" rid="B38">Wang et al., 2017</xref>). The increasing human activity stress in LMRB has caused streamflow discharge to increase from a pristine level to a much higher one by the early 2010s then to decline following a decrease in the human activity stress (<xref ref-type="bibr" rid="B26">Song et al., 2020</xref>). To quantitatively assess the impacts of climatic variation and human activities on streamflow in the LMRB, numerous methods such as empirical statistical analysis, multi-parameter vulnerability assessment, and hydrological modeling have been employed (<xref ref-type="bibr" rid="B39">Wu et al., 2016</xref>; <xref ref-type="bibr" rid="B36">Wang et al., 2019</xref>). <xref ref-type="bibr" rid="B18">Li et al. (2020)</xref> used the Mann-Kendall test and double cumulative curve method to determine the varying trend in the annual streamflow of the Mun River into the Mekong River from 1980 to 2018. Their results showed that the significant reduction in forest area and slight reductions in evaporation and farmland area since 1999 helped increase the streamflow of the Mun River. Gui et al. (2021) used the multi-parameter vulnerability assessment method to quantitatively assess the impacts of natural and anthropogenic interferences on water resources in the Lancang River Basin (LRB) from 1998 to 2014 and identified that the southeast region of LRB especially Dali area was mainly influenced by human activities. Although the method used by Gui et al. (2021) is able to directly quantify the contributions of climatic and human activities factors, the spatial differences of these factors in the very large LMRB may lead to inaccurate results. The hydrological simulation methods, typically the Soil &#x26; Water Assessment Tool (SWAT), Variable Infiltration Capacity (VIC), Simple Hydrological Model (SIMHYD), and Geomorphology-Based Hydrological Modelling (GBHM), all provide a more detailed illustration of hydrological cycle for river basins with diverse spatial patterns (<xref ref-type="bibr" rid="B38">Wang et al., 2017</xref>; <xref ref-type="bibr" rid="B41">Yun et al., 2020</xref>). Using a SWAT model, <xref ref-type="bibr" rid="B16">Li et al. (2021)</xref> quantitatively assessed the effects of climate change on streamflow in the Mun River under the RCP 2.6, 4.5, and 8.5 scenarios. <xref ref-type="bibr" rid="B35">Tatsumi et al. (2015)</xref> adopted VIC model to study the influence of agricultural irrigation water use on streamflow of the Mekong River. <xref ref-type="bibr" rid="B38">Wang et al. (2017)</xref> combined the large scale distributed hydrological GBHM with a simple reservoir regulation model to study the influence of dam construction and climate change on floods in the LMRB. While those modelling studies greatly facilitated quantitative evaluation of the factors affecting streamflow variation in LMRB, the Lancang and Mekong River Basins have not been treated as an integrated one large basin, and the temporal and spatial connections between the parts and the whole LMRB have been ignored in most cases. Furthermore, previous studies mainly focused on a certain aspect of the impact of human activities, such as reservoirs and irrigation, generally covered relatively short periods of variation and used insufficient model calibration data.</p>
<p>Aiming at quantitatively evaluating the impact of climate change and human activities on streamflow over a much long period and in a form well integrating the different parts of the entire LMRB, this study fused multi-source data, including long series of meteorological site and grid, land use types, and soil data and more, and then built a meteorological-soil-hydrological database. Importantly, a SWAT hydrological model was constructed by taking into account of the spatial heterogeneity in climatic and human activities factors across the entire LMRB and consequently the contributions of climatic and human activities factors in the streamflow changes over different parts of LMRB were quantified.</p>
</sec>
<sec id="s2">
<title>2 Research area and methods</title>
<sec id="s2-1">
<title>2.1 Research area</title>
<p>As the seventh largest river in the world, the Lancang-Mekong River (LMR) is shared by six nations in Southeast Asia (<xref ref-type="fig" rid="F1">Figure 1</xref>, <xref ref-type="bibr" rid="B20">Liu et al., 2021</xref>). It originates from the Tibetan Plateau in China, flows through Myanmar, Lao People&#x2019;s Democratic Republic, Thailand, and Cambodia, and draining into the South China Sea from the Mekong Delta in Vietnam (<xref ref-type="bibr" rid="B11">Gupta, 2022</xref>). The LMRB is generally divided into the upper (Lancang River Basin in China, LRB) and lower parts (Mekong River Basin, MRB). The entire length of the LMR is 4,880&#xa0;km with a total area of 795,000&#xa0;km<sup>2</sup> (<xref ref-type="bibr" rid="B5">Dong et al., 2022</xref>). The main stream of the Lancang River is 2,161&#xa0;km long and covers a drainage area of 165,000&#xa0;km<sup>2</sup>, accounting for 20.7% of the total area of the LMRB. The main stream of the Mekong River is 2,668&#xa0;km long and covers a drainage area of approximately 630,000&#xa0;km<sup>2</sup>, accounting for 79.3% of the whole LMRB.</p>
<fig id="F1" position="float">
<label>FIGURE 1</label>
<caption>
<p>The Lancang-Mekong River Basin and the distribution of hydrological stations.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g001.tif"/>
</fig>
<p>The LMRB comprises high mountains (4,500&#xa0;m elevation) and deep gullies in the upstream area, relatively flat terrain with medium-sized mountains in the midstream, and wide valleys and large tributary river systems in the downstream area, and is characterized by unique and complex hydrologic, climatic, and physiographic features (<xref ref-type="bibr" rid="B7">Fan and Luo, 2019</xref>). The distribution of mean annual precipitation over the whole basin follows a distinct north-to-south and west-to-east gradient. Annual precipitation can be as little as 600&#xa0;mm in the north of the Jiuzhou area in the Tibetan Plateau, and as much as 1,700&#xa0;mm in the southern mountains of the Jiu-Yun area. Regulated by the global monsoon system, the rain-soaked uplands in the V-S area receive the most precipitation (3,000&#xa0;mm) and the semi-arid Khorat Plateau in the west receives the least (1,000 to 1,600&#xa0;mm). The LMRB has two distinct seasons: a wet (May&#x2013;October) and a dry season (November&#x2013;April). Although interannual variation of monsoon precipitation is high, the wet season can contribute more than 80% of the annual regional precipitation. During the dry season, the basin is influenced mostly by dry air from the northeastern land (<xref ref-type="bibr" rid="B25">R&#xe4;s&#xe4;nen and Kummu, 2013</xref>). The annual temperature in the LMRB is mainly influenced by latitude. The average temperatures in Jiuzhou, Jiu-Yun, Yun-V, and V-S are 2.5&#xb0;C, 15.5&#xb0;C, 26&#xb0;C, and 30&#xb0;C, respectively. Population density also increases with latitude from north to south.</p>
<p>In this study, the Mekong Estuary Delta region was not given a detailed investigation due to the lack of detailed hydrological data. According to the locations of Jiuzhou, Yunjinghong, Vientiane and Stung Treng hydrological stations (<xref ref-type="fig" rid="F1">Figure 1</xref>), four parts of areas were zoned as Jiuzhou and above (Jiuzhou) area, Jiuzhou-Yunjinghong (Jiu-Yun) area, Yunjinghong-Vientiane (Yun-V) area and Vientiane-Stung Treng (V-S) area.</p>
</sec>
<sec id="s2-2">
<title>2.2 Data collection</title>
<p>The number of observation stations and data series in the LMRB, especially in the MRB, are insufficient, and the data sources and scales are inconsistent, which causes great difficulties for systematic and subsequent modeling analyses. To solve this problem, this study adopted the data input format as site in LRB &#x2b; grid in MRB, with each observation and grid station containing one group of data, including precipitation, temperature, evapotranspiration, DEM, soil and land cover data. Specific data sources are as follows:<list list-type="simple">
<list-item>
<p>1) Meteorological data</p>
</list-item>
</list>
</p>
<p>Daily observed meteorological data such as precipitation, temperature, wind and solar radiation data of the LRB (<xref ref-type="fig" rid="F2">Figure 2</xref>) were collected from the Chinese meteorological data sharing service system (<ext-link ext-link-type="uri" xlink:href="http://data.cma.cn/">http://data.cma.cn/</ext-link>). Daily grid meteorological data of the MRB were collected from Mekong River Commission (MRC, <ext-link ext-link-type="uri" xlink:href="https://www.mrcmekong.org/">https://www.mrcmekong.org/</ext-link>) and the Asian Precipitation-Highly-Resolved Observational Data Integration Towards Evaluation (APHRODITE, 0.25&#xb0; &#x2179; 0.25&#xb0;, <ext-link ext-link-type="uri" xlink:href="http://aphrodite.st.hirosaki-u.ac.jp/download/">http://aphrodite.st.hirosaki-u.ac.jp/download/</ext-link>), respectively. To compensate for the lack of APHRODITE data at the maximum and minimum temperature, this study used the Global Surface Summary of the Day (GSOD, <ext-link ext-link-type="uri" xlink:href="https://data.noaa.gov/dataset/dataset/global-surface-summary-of-the-day-gsod">https://data.noaa.gov/dataset/dataset/global-surface-summary-of-the-day-gsod</ext-link>) data and Global Historical Climatology Network data (GHCND, <ext-link ext-link-type="uri" xlink:href="https://www.ncei.noaa.gov/products/land-based-station/global-historical-climatology-network-daily">https://www.ncei.noaa.gov/products/land-based-station/global-historical-climatology-network-daily</ext-link>) from NOAA (National Oceanic Atmospheric Adminstration). Evapotranspiration was calculated using the Penman-Monteith equation based on weather generator in SWAT model with default parameters setting.<list list-type="simple">
<list-item>
<p>2) Hydrological and topographic data</p>
</list-item>
</list>
</p>
<fig id="F2" position="float">
<label>FIGURE 2</label>
<caption>
<p>Distribution of meteorological stations in the Lancang-Mekong River Basin.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g002.tif"/>
</fig>
<p>The streamflow data were provided by Yunnan University and <xref ref-type="bibr" rid="B21">the Mekong River Commission (MRC)</xref>. The data series duration of Jiuzhou, Yunjinghong, Vientiane and Stung Treng station are 1961&#x2013;2010, 1961&#x2013;2014, 1961&#x2013;2006 and 1961&#x2013;2015, respectively. The geographical data, such as the 1&#xa0;km &#xd7; 1&#xa0;km raster data, was extracted and resampled from 90&#xa0;m resolution digital elevation model (DEM) data provided by the USGS (available from <ext-link ext-link-type="uri" xlink:href="http://www.usgs.gov">http://www.usgs.gov</ext-link>).<list list-type="simple">
<list-item>
<p>3) Land cover and soil data</p>
</list-item>
</list>
</p>
<p>The land use and land cover data of the LRB (1980&#x2013;2015, per 5 years) were produced by the Institute of Geographical Sciences and Resources, Chinese Academy of Sciences, and the similar data of the MRB were downloaded and resampled from the Servir-Mekong dataset from 1987 to 2015 (available from <ext-link ext-link-type="uri" xlink:href="https://servir.adpc.net">https://servir.adpc.net</ext-link>). Soil data in LMRB was obtained from the Harmonized World Soil Database (HWSD).</p>
</sec>
<sec id="s2-3">
<title>2.3 Materials and methods</title>
<sec id="s2-3-1">
<title>2.3.1 Construction of SWAT model in the study area</title>
<p>SWAT (Soil and Water Assessment Tool) model is a semi-distributed watershed hydrological model developed by Blackland Research and Extension Center of USDA in the early 1990s, which is mainly used to simulate the process of land and water surface cycle (<xref ref-type="bibr" rid="B9">Gassman et al., 2007</xref>). The land surface part can be divided into the process of streamflow generation and slope confluence, while the water surface part is the river network confluence (<xref ref-type="bibr" rid="B24">Paiva et al., 2011</xref>; <xref ref-type="bibr" rid="B30">Tan et al., 2022</xref>.).</p>
<p>Database including the spatial and attribute data is necessary to be bulit first. Spatial data such as DEM, the location of meteorological stations, land cover types, and soil distribution map, and attribute data such as meteorological data series and soil attribute data were edited and input into the SWAT model, respectively. Based on the established LMRB database, the LMRB system network was generated by extracting DEM data from the database. Combined with soil, land cover types, and slope values, the LMRB was divided into 35 sub-basins. Considering the calculation accuracy and processing speed, 35 sub-basins are further divided into 311 HRUs (hydrological response units) with an average area of 2,000&#xa0;km<sup>2</sup>, as shown in <xref ref-type="fig" rid="F3">Figure 3</xref>.</p>
<fig id="F3" position="float">
<label>FIGURE 3</label>
<caption>
<p>Sub-basin division by SWAT hydrological model in Lancang-Mekong River Basin <bold>(A)</bold> Watershed soil types map. <bold>(B)</bold> HRUs division map of sub-basins. <bold>(C)</bold> Watershed land use types map.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g003.tif"/>
</fig>
</sec>
<sec id="s2-3-2">
<title>2.3.2 Calibration and validation of SWAT model</title>
<p>The calibration and validation periods of the SWAT model were from 1961 (3 years warm-up period) to 1980, and 1981 to 2015, respectively. For validation, 35 years of data was considered sufficiently long (<xref ref-type="bibr" rid="B19">Liu et al., 2010</xref>; <xref ref-type="bibr" rid="B42">Zhang et al., 2012</xref>), when compared with other studies that used 2&#x2013;3 years of data. The land cover data of 1990, which was less affected by human activities, was input to the model as the underlying surface data for the restoration of natural streamflow. This study adopted the step-by-step calibration principle to validate the streamflow data from upstream to downstream and LH-OAT (Latin-Hypercube &#x26; One Factor-At-a-Time) method to analyze the sensitivity of the main parameters. Method LH-OAT assumes that there are <italic>p</italic> parameters to be analyzed in the model, which are divided into N layers in space according to the LH (Latin-Hypercube) sampling idea, and then taking a sampling in each layer. After that, parameters of each LH sampling parameter group are changed according to the OAT (one Factor-at-a-time) method, and each small change is recorded as <italic>GS</italic>
<sub>
<italic>i</italic>
</sub> (global sensitivity). The sensitivity classification and the the detailed calibration process of the <italic>GS</italic>
<sub>
<italic>i</italic>
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<mml:msub>
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<mml:mrow>
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<mml:mrow>
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<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
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<mml:mrow>
<mml:mn>1</mml:mn>
<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
<mml:mo>,</mml:mo>
<mml:mo>&#x2026;</mml:mo>
<mml:mo>,</mml:mo>
<mml:msub>
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<mml:mrow>
<mml:mi>i</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
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<mml:msub>
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<mml:mrow>
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<mml:mrow>
<mml:mi>p</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mo>&#x2b;</mml:mo>
<mml:mi>M</mml:mi>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:msub>
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<mml:mrow>
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<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
<mml:mo>,</mml:mo>
<mml:mo>&#x2026;</mml:mo>
<mml:mo>,</mml:mo>
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<mml:mrow>
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<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
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<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mrow>
<mml:mo>/</mml:mo>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:mrow>
</mml:mfrac>
<mml:mrow>
<mml:mrow>
<mml:mo>&#x2206;</mml:mo>
<mml:msub>
<mml:mi>e</mml:mi>
<mml:mrow>
<mml:mi>i</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
<mml:mrow>
<mml:mo>/</mml:mo>
</mml:mrow>
<mml:msub>
<mml:mi>e</mml:mi>
<mml:mrow>
<mml:mi>i</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>k</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfrac>
</mml:mrow>
</mml:math>
<label>(1)</label>
</disp-formula>
</p>
<table-wrap id="T1" position="float">
<label>TABLE 1</label>
<caption>
<p>Sensitivity classification.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="center">Class</th>
<th align="center">Sensitivity range</th>
<th align="center">Sensitive degree</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td align="center">1</td>
<td align="center">0.00&#x2264; &#x2223;<italic>GS</italic>
<sub>
<italic>i</italic>
</sub>&#x2223;&#x3c;0.05</td>
<td align="center">Insensitivity</td>
</tr>
<tr>
<td align="center">2</td>
<td align="center">0.05&#x2264; &#x2223;<italic>GS</italic>
<sub>
<italic>i</italic>
</sub>&#x2223;&#x3c;0.20</td>
<td align="center">General sensitive</td>
</tr>
<tr>
<td align="center">3</td>
<td align="center">0.20&#x2264; &#x2223;<italic>GS</italic>
<sub>
<italic>i</italic>
</sub>&#x2223;&#x3c;1.00</td>
<td align="center">Sensitive</td>
</tr>
<tr>
<td align="center">4</td>
<td align="center">1.00&#x2264; &#x2223;<italic>GS</italic>
<sub>
<italic>i</italic>
</sub>&#x2223;</td>
<td align="center">Supersensitive</td>
</tr>
</tbody>
</table>
</table-wrap>
<p>
<italic>M</italic>(&#x2a;) is the indicator function of the <italic>k</italic>th LH sampling set; <italic>e</italic>
<sub>
<italic>i,k</italic>
</sub> is the value of the <italic>i</italic>th parameter in the <italic>k</italic>th LH sampling layer; and <italic>&#x394;e</italic>
<sub>
<italic>i,k</italic>
</sub> is some disturbance of parameter <italic>e</italic>
<sub>
<italic>i,k</italic>
</sub>.</p>
<p>Before applying the SWAT model to reconstruct natural streamflow in the LMR, the simulation capability of the model needs to be evaluated in the study area. The coefficient of determination (<italic>R</italic>
<sup>
<italic>2</italic>
</sup>) and the Nash&#x2013;Sutcliffe efficiency (<italic>NSE</italic>) were used to evaluate the merits of the SWAT model. <italic>R</italic>
<sup>
<italic>2</italic>
</sup> calculates the linear correlations between observed and modelled data from SWAT. <italic>NSE</italic> evaluates the SWAT model&#x2019;s goodness of fit. <italic>R</italic>
<sup>
<italic>2</italic>
</sup> and <italic>NSE</italic> are defined as follows:<disp-formula id="e2">
<mml:math id="m6">
<mml:mrow>
<mml:mi>N</mml:mi>
<mml:mi>S</mml:mi>
<mml:mi>E</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mn>1</mml:mn>
<mml:mo>&#x2212;</mml:mo>
<mml:mfrac>
<mml:mrow>
<mml:msubsup>
<mml:mstyle displaystyle="true">
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<mml:mn>0</mml:mn>
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<mml:mi>S</mml:mi>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:msup>
</mml:mrow>
<mml:mrow>
<mml:msubsup>
<mml:mstyle displaystyle="true">
<mml:mo>&#x2211;</mml:mo>
</mml:mstyle>
<mml:mrow>
<mml:mi>i</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mn>1</mml:mn>
</mml:mrow>
<mml:mi>n</mml:mi>
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<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:msup>
</mml:mrow>
</mml:mfrac>
</mml:mrow>
</mml:math>
<label>(2)</label>
</disp-formula>
<disp-formula id="e3">
<mml:math id="m7">
<mml:mrow>
<mml:msup>
<mml:mi>R</mml:mi>
<mml:mn>2</mml:mn>
</mml:msup>
<mml:mo>&#x3d;</mml:mo>
<mml:mfrac>
<mml:mrow>
<mml:msubsup>
<mml:mo>&#x2211;</mml:mo>
<mml:mrow>
<mml:mi>d</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mn>1</mml:mn>
</mml:mrow>
<mml:mi>n</mml:mi>
</mml:msubsup>
<mml:msup>
<mml:mrow>
<mml:mfenced open="[" close="]" separators="|">
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<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mn>0</mml:mn>
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</mml:msub>
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<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mn>0</mml:mn>
<mml:mo>,</mml:mo>
<mml:mi>d</mml:mi>
</mml:mrow>
</mml:msub>
<mml:mo>&#x2212;</mml:mo>
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<mml:mi>Q</mml:mi>
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<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:msup>
</mml:mrow>
<mml:mrow>
<mml:msubsup>
<mml:mo>&#x2211;</mml:mo>
<mml:mrow>
<mml:mi>d</mml:mi>
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</mml:mrow>
<mml:mi>n</mml:mi>
</mml:msubsup>
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<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mn>0</mml:mn>
<mml:mo>,</mml:mo>
<mml:mi>d</mml:mi>
</mml:mrow>
</mml:msub>
<mml:mo>&#x2212;</mml:mo>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
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<mml:mo>,</mml:mo>
<mml:mi>a</mml:mi>
<mml:mi>v</mml:mi>
<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:msup>
<mml:msubsup>
<mml:mo>&#x2211;</mml:mo>
<mml:mrow>
<mml:mi>i</mml:mi>
<mml:mo>&#x3d;</mml:mo>
<mml:mn>1</mml:mn>
</mml:mrow>
<mml:mi>n</mml:mi>
</mml:msubsup>
<mml:msup>
<mml:mrow>
<mml:mfenced open="(" close=")" separators="|">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mi>s</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>d</mml:mi>
</mml:mrow>
</mml:msub>
<mml:mo>&#x2212;</mml:mo>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mi>s</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>a</mml:mi>
<mml:mi>v</mml:mi>
<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:mfenced>
</mml:mrow>
<mml:mn>2</mml:mn>
</mml:msup>
</mml:mrow>
</mml:mfrac>
</mml:mrow>
</mml:math>
<label>(3)</label>
</disp-formula>Where <inline-formula id="inf5">
<mml:math id="m8">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mn>0</mml:mn>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf6">
<mml:math id="m9">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mi>s</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> are the measured and simulated flow (m<sup>3</sup>/s) of time step <italic>d</italic>, respectively. <inline-formula id="inf7">
<mml:math id="m10">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mn>0</mml:mn>
<mml:mo>,</mml:mo>
<mml:mi>a</mml:mi>
<mml:mi>v</mml:mi>
<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf8">
<mml:math id="m11">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mrow>
<mml:mi>s</mml:mi>
<mml:mo>,</mml:mo>
<mml:mi>a</mml:mi>
<mml:mi>v</mml:mi>
<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> are the measured and simulated average flow (m<sup>3</sup>/s) during simulation, respectively.</p>
<p>
<xref ref-type="fig" rid="F4">Figure 4</xref> shows the observed and simulated monthly streamflow of Jiuzhou, Yunjinghong, Vientiane, and Stung Treng stations during the calibration and validation periods. The NSE values of the calibration period of the four stations were 0.75, 0.80, 0.88, and 0.75, respectively. During the validation period, the NSE values were 0.71,0.75, 0.82, and 0.72, respectively. The <italic>R</italic>
<sup>2</sup> values were all &#x3e;0.84. Based on the recommendations of previous research, a hydrological simulation is satisfactory when NSE&#x3e;0.50 and <italic>R</italic>
<sup>2</sup> &#x3e;0.6.</p>
<fig id="F4" position="float">
<label>FIGURE 4</label>
<caption>
<p>Calibration and validation results of the SWAT model for the monthly average streamflow at Jiuzhou <bold>(A)</bold>, Yunjinghong <bold>(B)</bold>, Vientiane <bold>(C)</bold>, and Stung Treng <bold>(D)</bold> stations based on the flow data measured during 1961&#x2013;2015 in line with the monthly average precipitation (the blue column with the right vertical axis).</p>
</caption>
<graphic xlink:href="feart-10-1024037-g004.tif"/>
</fig>
</sec>
<sec id="s2-3-3">
<title>2.3.3 Assessment of the contributions of climatic variations and human activities to streamflow changes</title>
<p>According to the calculation principle of the hydrological model, the difference in observed streamflow between the reference and change period is <inline-formula id="inf9">
<mml:math id="m12">
<mml:mrow>
<mml:mo>&#x394;</mml:mo>
<mml:mi>Q</mml:mi>
</mml:mrow>
</mml:math>
</inline-formula>. This parameter represents a combination of climate change and human activity. The difference in natural streamflow (simulated streamflow) between the two periods, <inline-formula id="inf10">
<mml:math id="m13">
<mml:mrow>
<mml:mo>&#x394;</mml:mo>
<mml:msub>
<mml:mi>Q</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>, reflects the impact of climate change. Therefore, the contributions of both climatic variations and human activities to streamflow changes were quantified by using the following formulas:<disp-formula id="e4">
<mml:math id="m14">
<mml:mrow>
<mml:mo>&#x2206;</mml:mo>
<mml:mi>Q</mml:mi>
<mml:mo>&#x3d;</mml:mo>
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</mml:msub>
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<label>(4)</label>
</disp-formula>
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<label>(5)</label>
</disp-formula>
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<label>(6)</label>
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<mml:mn>100</mml:mn>
<mml:mo>%</mml:mo>
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<label>(11)</label>
</disp-formula>where <inline-formula id="inf11">
<mml:math id="m22">
<mml:mrow>
<mml:mo>&#x394;</mml:mo>
<mml:mi>Q</mml:mi>
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</mml:mrow>
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</mml:mrow>
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</mml:math>
</inline-formula> represents the change amount of observed streamflow in the variation period relative to the reference period. <inline-formula id="inf12">
<mml:math id="m23">
<mml:mrow>
<mml:msub>
<mml:mi>Q</mml:mi>
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</inline-formula> and <inline-formula id="inf13">
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<mml:mi>e</mml:mi>
</mml:mrow>
</mml:msub>
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</mml:mrow>
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</inline-formula> represent the observed mean streamflow during variation and the reference period, respectively. <inline-formula id="inf14">
<mml:math id="m25">
<mml:mrow>
<mml:mo>&#x394;</mml:mo>
<mml:msub>
<mml:mi>Q</mml:mi>
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</inline-formula> and <inline-formula id="inf15">
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<mml:mi>Q</mml:mi>
<mml:mi>c</mml:mi>
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</mml:mrow>
</mml:math>
</inline-formula> represent the impact amount from human activities and climate change on streamflow changes, respectively. <inline-formula id="inf16">
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</inline-formula> represents the impact amount from land use change, <inline-formula id="inf17">
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</mml:math>
</inline-formula> represents the impact amount from other forms of human activities except land use, such as reservoir regulation, inter-basin water diversion, <italic>etc.</italic> <inline-formula id="inf18">
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</inline-formula>, <inline-formula id="inf19">
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</inline-formula>, and <inline-formula id="inf20">
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</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> are the contributions of climatic variations, human activities, and land use to streamflow changes, respectively.</p>
</sec>
</sec>
</sec>
<sec sec-type="results|discussion" id="s3">
<title>3 Results and discussion</title>
<sec id="s3-1">
<title>3.1 Reference and change periods judgement</title>
<p>The cumulative anomalies of streamflow series at Jiuzhou, Yunjinghong, Vientiane, and Stung Treng hydrological stations are shown in <xref ref-type="fig" rid="F5">Figure 5</xref>, and an obvious decrease in the annual streamflow can be noticed from 1966 to 1980&#xa0;at Jiuzhou (<xref ref-type="fig" rid="F5">Figure 5A</xref>), from 1986 to 2000&#xa0;at Yunjinghong (<xref ref-type="fig" rid="F5">Figure 5B</xref>), and from 1986 to 1999&#xa0;at Vientiane and Stung Treng stations (<xref ref-type="fig" rid="F5">Figures 5C,D</xref>). Hence, the impact of human activities on streamflow from 1961 to 1980 can be regarded as at a low level and so the period is taken as the reference period in this study, while 1986 can be regarded as the sudden changing year in the annual streamflow series at these four stations. This division is consistent with the findings of other studies (e.g., <xref ref-type="bibr" rid="B31">Tang et al., 2014</xref>; <xref ref-type="bibr" rid="B38">Wang et al., 2017</xref>). Therefore, we divided the change periods of annual streamflow at four stations into two periods: at Jiuzhou, periods A<sub>1</sub> (1986&#x2013;1997) and B<sub>1</sub> (1998&#x2013;2010); at Yunjinghong, periods A<sub>2</sub> (1986&#x2013;2000) and B<sub>2</sub> (2001&#x2013;2014); at Vientiane, periods A<sub>3</sub> (1986&#x2013;1999) and B<sub>3</sub> (2000&#x2013;2006); and at Stung Treng, periods A<sub>4</sub> (1986&#x2013;1999) and B<sub>4</sub> (2000&#x2013;2015). Correspondingly, the reference periods were named Base<sub>1</sub>, Base<sub>2</sub>, Base<sub>3</sub>, and Base<sub>4</sub>, respectively.</p>
<fig id="F5" position="float">
<label>FIGURE 5</label>
<caption>
<p>The cumulative anomaly variation of annual streamflow at Jiuzhou <bold>(A)</bold>, Yunjinghong <bold>(B)</bold>, Vientiane <bold>(C)</bold>, and Stung Treng <bold>(D)</bold> station.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g005.tif"/>
</fig>
</sec>
<sec id="s3-2">
<title>3.2 Trends of hydro-climatic factors during 1961&#x2013;2015</title>
<p>The yearly and seasonal average streamflow at Jiuzhou, Yunjinghong, Vientiane, and Stung Treng stations in different periods are listed in <xref ref-type="table" rid="T2">Table 2</xref>. At the annual timescale, the streamflow volume at the four stations showed an obvious increase from upstream to downstream. By comparison with the volume of the streamflow in base periods, the volume of the streamflow increased in the changing period B<sub>1</sub> at Jiuzhou station, B<sub>3</sub> at Vientiane station, and B<sub>4</sub> at Stung Treng station, and yet an obvious decrease in the changing period B<sub>2</sub> at Yunjinghong station, A<sub>3</sub> at Vientiane station, and A<sub>4</sub> at Stung Treng station. At the seasonal timescale, the streamflow volume at the four stations showed little change in the dry season, but a much more dramatic change in the wet season.</p>
<table-wrap id="T2" position="float">
<label>TABLE 2</label>
<caption>
<p>The results of Mann-Kendall trend tests for the hydro-climatic data in Lancang-Mekong River.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th rowspan="3" align="left">Station</th>
<th rowspan="3" align="left">Timescale</th>
<th colspan="6" align="center">Precipitation (mm/10a)</th>
<th colspan="6" align="center">Streamflow (10<sup>9</sup>m&#xb3;/10a)</th>
</tr>
<tr>
<th colspan="2" align="center">Basis period</th>
<th colspan="2" align="center">Change period A</th>
<th colspan="2" align="center">Change period B</th>
<th colspan="2" align="center">Basis period</th>
<th colspan="2" align="center">Change period A</th>
<th colspan="2" align="center">Change period B</th>
</tr>
<tr>
<th align="center">Z value</th>
<th align="center">Trend</th>
<th align="center">Z value</th>
<th align="center">Trend</th>
<th align="center">Z value</th>
<th align="center">Trend</th>
<th align="center">Z value</th>
<th align="center">Trend</th>
<th align="center">Z value</th>
<th align="center">Trend</th>
<th align="center">Z value</th>
<th align="center">Trend</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td rowspan="3" align="left">Jiuzhou area</td>
<td align="left">Annual</td>
<td align="center">&#x2212;0.22</td>
<td align="center">&#x2212;8.00</td>
<td align="center">0.12</td>
<td align="center">3.47</td>
<td align="center">&#x2212;2.26&#x2a;&#x2a;</td>
<td align="center">&#x2212;91.39</td>
<td align="center">&#x2212;1.07</td>
<td align="center">&#x2212;3.30</td>
<td align="center">0.37</td>
<td align="center">1.57</td>
<td align="center">&#x2212;1.66&#x2a;</td>
<td align="center">&#x2212;6.96</td>
</tr>
<tr>
<td align="left">Dry season</td>
<td align="center">1.91</td>
<td align="center">14.40</td>
<td align="center">0.70</td>
<td align="center">8.41</td>
<td align="center">0.18</td>
<td align="center">7.80</td>
<td align="center">&#x2212;0.55</td>
<td align="center">&#x2212;0.81</td>
<td align="center">1.36</td>
<td align="center">0.48</td>
<td align="center">&#x2212;1.40</td>
<td align="center">&#x2212;0.67</td>
</tr>
<tr>
<td align="left">Wet season</td>
<td align="center">&#x2212;0.29</td>
<td align="center">&#x2212;21.15</td>
<td align="center">0.04</td>
<td align="center">-4.94</td>
<td align="center">&#x2212;2.87</td>
<td align="center">&#x2212;99.19</td>
<td align="center">&#x2212;0.81</td>
<td align="center">&#x2212;3.34</td>
<td align="center">0.37</td>
<td align="center">1.11</td>
<td align="center">&#x2212;1.65&#x2a;</td>
<td align="center">&#x2212;6.41</td>
</tr>
<tr>
<td rowspan="3" align="left">Jiu-Yun area</td>
<td align="left">Annual</td>
<td align="center">&#x2212;1.20</td>
<td align="center">&#x2212;35.88</td>
<td align="center">1.98&#x2a;&#x2a;</td>
<td align="center">78.27</td>
<td align="center">&#x2212;2.41</td>
<td align="center">&#x2212;133.81</td>
<td align="center">&#x2212;1.46</td>
<td align="center">&#x2212;4.64</td>
<td align="center">0.99</td>
<td align="center">5.73</td>
<td align="center">&#x2212;2.52&#x2a;&#x2a;</td>
<td align="center">&#x2212;13.56</td>
</tr>
<tr>
<td align="left">Dry season</td>
<td align="center">&#x2212;0.29</td>
<td align="center">&#x2212;7.29</td>
<td align="center">0.00</td>
<td align="center">3.74</td>
<td align="center">&#x2212;0.88</td>
<td align="center">&#x2212;19.33</td>
<td align="center">&#x2212;0.98</td>
<td align="center">&#x2212;0.50</td>
<td align="center">0.69</td>
<td align="center">0.93</td>
<td align="center">&#x2212;0.99</td>
<td align="center">&#x2212;1.35</td>
</tr>
<tr>
<td align="left">Wet season</td>
<td align="center">&#x2212;0.94</td>
<td align="center">&#x2212;28.59</td>
<td align="center">1.68&#x2a;</td>
<td align="center">74.53</td>
<td align="center">&#x2212;2.08&#x2a;&#x2a;</td>
<td align="center">&#x2212;114.48</td>
<td align="center">&#x2212;1.20</td>
<td align="center">&#x2212;4.17</td>
<td align="center">0.89</td>
<td align="center">4.90</td>
<td align="center">&#x2212;0.88</td>
<td align="center">&#x2212;3.27</td>
</tr>
<tr>
<td rowspan="3" align="left">Yun-V area</td>
<td align="left">Annual</td>
<td align="center">0.55</td>
<td align="center">50.45</td>
<td align="center">0.99</td>
<td align="center">143.61</td>
<td align="center">&#x2212;0.30</td>
<td align="center">&#x2212;92.65</td>
<td align="center">&#x2212;0.49</td>
<td align="center">&#x2212;3.23</td>
<td align="center">1.64</td>
<td align="center">20.95</td>
<td align="center">&#x2212;0.90</td>
<td align="center">&#x2212;80.51</td>
</tr>
<tr>
<td align="left">Dry season</td>
<td align="center">0.36</td>
<td align="center">1.93</td>
<td align="center">0.33</td>
<td align="center">26.09</td>
<td align="center">0.00</td>
<td align="center">38.57</td>
<td align="center">&#x2212;1.65&#x2a;</td>
<td align="center">&#x2212;2.87</td>
<td align="center">0.66</td>
<td align="center">1.73</td>
<td align="center">&#x2212;1.80&#x2a;</td>
<td align="center">&#x2212;14.87</td>
</tr>
<tr>
<td align="left">Wet season</td>
<td align="center">0.49</td>
<td align="center">48.55</td>
<td align="center">0.99</td>
<td align="center">117.52</td>
<td align="center">&#x2212;0.60</td>
<td align="center">&#x2212;131.22</td>
<td align="center">&#x2212;0.42</td>
<td align="center">&#x2212;0.36</td>
<td align="center">1.86&#x2a;</td>
<td align="center">19.22</td>
<td align="center">&#x2212;1.20</td>
<td align="center">&#x2212;65.64</td>
</tr>
<tr>
<td rowspan="3" align="left">V-S area</td>
<td align="left">Annual</td>
<td align="center">1.01</td>
<td align="center">18.60</td>
<td align="center">1.31</td>
<td align="center">130.97</td>
<td align="center">&#x2212;0.77</td>
<td align="center">&#x2212;108.89</td>
<td align="center">&#x2212;1.72&#x2a;</td>
<td align="center">&#x2212;31.87</td>
<td align="center">1.09</td>
<td align="center">54.66</td>
<td align="center">&#x2212;0.86</td>
<td align="center">&#x2212;32.95</td>
</tr>
<tr>
<td align="left">Dry season</td>
<td align="center">0.03</td>
<td align="center">4.11</td>
<td align="center">2.19&#x2a;&#x2a;</td>
<td align="center">117.17</td>
<td align="center">0.50</td>
<td align="center">10.07</td>
<td align="center">&#x2212;1.33</td>
<td align="center">&#x2212;4.69</td>
<td align="center">1.65&#x2a;</td>
<td align="center">17.18</td>
<td align="center">&#x2212;0.41</td>
<td align="center">&#x2212;2.04</td>
</tr>
<tr>
<td align="left">Wet season</td>
<td align="center">0.23</td>
<td align="center">14.49</td>
<td align="center">0.00</td>
<td align="center">13.80</td>
<td align="center">&#x2212;1.13</td>
<td align="center">&#x2212;118.95</td>
<td align="center">&#x2212;1.52</td>
<td align="center">&#x2212;27.19</td>
<td align="center">0.66</td>
<td align="center">37.48</td>
<td align="center">&#x2212;0.99</td>
<td align="center">&#x2212;30.91</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn>
<p>Note: &#x2a;, &#x2a;&#x2a; indicated that the data series passed significance test at &#x3b1;&#x3d;0.10 and 0.05 levels, respectively.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>The Mann-Kendall (M-K) non-parametric trend test method was used to conduct trend detection of streamflow at these four stations and precipitation in Jiuzhou, Jiu-Yun, Yun-V, and V-S areas on yearly and seasonal time scales from 1961 to 2015 (<xref ref-type="bibr" rid="B15">Kendall, 1975</xref>; <xref ref-type="bibr" rid="B40">Yanming et al., 2012</xref>; <xref ref-type="bibr" rid="B23">Noszczyk et al., 2017</xref>; <xref ref-type="bibr" rid="B22">Ning et al., 2021</xref>). The Z value, which is the result of M-K test, obeyed the standard normal distribution. A positive Z value indicates an increasing trend, while a negative Z value indicates a decreasing trend. The bilateral significance level test was used to test the monotonic trend of increase or decrease. If &#x7c;Z&#x7c; &#x3e; 1.645, 1.96, and 2.576, it shows that the time series have passed the confidence test of &#x3b1;&#x3d;0.01, 0.05, and 0.1 respectively. At the yearly time scale, the streamflow at Jiuzhou station and that in the basis period and A<sub>2</sub> of Yunjinghong station did not show any obvious trends. However, the streamflow in change period B<sub>2</sub> showed a significant decreasing trend (0.05 significance level). The total streamflow at Vientiane and Stung Treng stations showed decreasing and increasing trends, respectively. However, both showed an increasing trend first and then decreased in the changing periods. At the seasonal time scale, the variation trends of streamflow in the dry and wet seasons were basically consistent with that at the yearly time scale in each area. However, the streamflow had a significant downward trend at Vientiane station and a significant upward trend at Stung Treng station in the dry season, and a significant downward trend at Jiuzhou station and a significant upward trend at Vientiane station in the wet season, while other stations had little change.</p>
<p>According to the correlation calculation results, the correlated degree of annual average precipitation and streamflow reached 0.91 in Jiuzhou, 0.74 in Jiu-Yun, 0.65 in Yun-V, but only 0.17 in V-S. The streamflow trends were consistent with precipitation in the dry season (Nov-next Apr) throughout the four areas with more than 0.5 correlation values and less precipitation and streamflow variation than in the wet season. The streamflow trends were consistent with the annual precipitation variation in the wet season of the four areas and the correlation degree between regional average precipitation and streamflow gradually weakened from upstream to downstream.</p>
</sec>
<sec id="s3-3">
<title>3.3 Contributions of climate and human activities to streamflow changes</title>
<p>In terms of Formulas (<xref ref-type="disp-formula" rid="e4">4</xref>&#x2013;<xref ref-type="disp-formula" rid="e9">9</xref>) and the different scenarios setting for quantitative contribution analysis of the human activities and climate change in LMRB (<xref ref-type="table" rid="T3">Table 3</xref>), the contributions of climate change and human activities to streamflow change were quantified by comparing the observed and simulated streamflow during basic and change periods. The contributions of the two factors were analyzed at the yearly and seasonal time scales, respectively. The results are illustrated in <xref ref-type="fig" rid="F6">Figure 6</xref>.</p>
<table-wrap id="T3" position="float">
<label>TABLE 3</label>
<caption>
<p>Scenarios for quantitative analysis the contributions of human activities and climate change in Lancang-Mekong River Basin.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="left">Station</th>
<th align="center">Group</th>
<th align="center">Land use</th>
<th align="center">Station</th>
<th align="center">Group</th>
<th align="center">Land use</th>
<th align="center">Objectiive</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td rowspan="5" align="center">Jiuzhou</td>
<td align="center">Base<sub>1</sub>:1961&#x2013;1980</td>
<td align="center">L1990</td>
<td rowspan="5" align="center">Jiu-Yun</td>
<td align="center">Base<sub>2</sub>:1961&#x2013;1980</td>
<td align="center">L1990</td>
<td rowspan="2" align="center">
<inline-formula id="inf21">
<mml:math id="m32">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf22">
<mml:math id="m33">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">A<sub>1</sub>:1986&#x2013;1999</td>
<td align="center">L2000</td>
<td align="center">A<sub>2</sub>:1986&#x2013;2000</td>
<td align="center">L2000</td>
</tr>
<tr>
<td align="center">B<sub>1</sub>:2000&#x2013;2010</td>
<td align="center">L2010</td>
<td align="center">B<sub>2</sub>:2001&#x2013;2014</td>
<td align="center">L2010</td>
<td align="center">
<inline-formula id="inf23">
<mml:math id="m34">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf24">
<mml:math id="m35">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">A<sub>1</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:1986&#x2013;1999</td>
<td align="center">L1990</td>
<td align="center">A<sub>2</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:1986&#x2013;2000</td>
<td align="center">L1990</td>
<td align="center">
<inline-formula id="inf25">
<mml:math id="m36">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">B<sub>1</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:2000&#x2013;2010</td>
<td align="center">L1990</td>
<td align="center">B<sub>2</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:2001&#x2013;2014</td>
<td align="center">L1990</td>
<td align="center">
<inline-formula id="inf26">
<mml:math id="m37">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td rowspan="5" align="center">Yun-V</td>
<td align="center">Base<sub>3</sub>:1961&#x2013;1980</td>
<td align="center">L1990</td>
<td rowspan="5" align="center">V-S</td>
<td align="center">Base<sub>4</sub>:1961&#x2013;1980</td>
<td align="center">L1990</td>
<td/>
</tr>
<tr>
<td align="center">A<sub>3</sub>:1986&#x2013;1999</td>
<td align="center">L2000</td>
<td align="center">A<sub>4</sub>:1986&#x2013;1999</td>
<td align="center">L2000</td>
<td align="center">
<inline-formula id="inf27">
<mml:math id="m38">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf28">
<mml:math id="m39">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">B<sub>3</sub>:2000&#x2013;2006</td>
<td align="center">L2005</td>
<td align="center">B<sub>4</sub>:2000&#x2013;2015</td>
<td align="center">L2010</td>
<td align="center">
<inline-formula id="inf29">
<mml:math id="m40">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> and <inline-formula id="inf30">
<mml:math id="m41">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">A<sub>3</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:1986&#x2013;1999</td>
<td align="center">L1990</td>
<td align="center">A<sub>4</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:1986&#x2013;1999</td>
<td align="center">L1990</td>
<td align="center">
<inline-formula id="inf31">
<mml:math id="m42">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
<tr>
<td align="center">B<sub>3</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:2000&#x2013;2006</td>
<td align="center">L1990</td>
<td align="center">B<sub>4</sub>
<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>:2000&#x2013;2015</td>
<td align="center">L1990</td>
<td align="center">
<inline-formula id="inf32">
<mml:math id="m43">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>c</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn id="Tfn1">
<label>
<sup>a</sup>
</label>
<p>Base, A, B, A<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>.</p>
</fn>
<fn>
<p>And B<xref ref-type="table-fn" rid="Tfn1">
<sup>a</sup>
</xref>represent the period of different scenarios.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<fig id="F6" position="float">
<label>FIGURE 6</label>
<caption>
<p>Contributions of climatic variations and human activities to annual and seasonal streamflow changes in Jiuzhou <bold>(A)</bold>, Jiu-Yun <bold>(B)</bold>, Yun-V <bold>(C)</bold>, and V-S <bold>(D)</bold> area.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g006.tif"/>
</fig>
<p>At the yearly time scale, the contributions of human activities (<inline-formula id="inf33">
<mml:math id="m44">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>) to the streamflow changes, which maintained a low percentage, were 15.2% (1986&#x2013;1999) and 10.3% (2000&#x2013;2010), respectively, in Jiuzou area (<xref ref-type="fig" rid="F6">Figure 6A</xref>). More farmland and water conservancy projects were found in Jiu-Yun area, so the contributions of human activity accounted for 17.5% in 1986&#x2013;2000 and 17.1% in 2001&#x2013;2014 (<xref ref-type="fig" rid="F6">Figure 6B</xref>), which was slightly more than that in Jiuzhou area. The contributions of human activity in Yun-V (<xref ref-type="fig" rid="F6">Figure 6C</xref>) and V-S (<xref ref-type="fig" rid="F6">Figure 6D</xref>) areas were much greater after 1999 (32.4%, 59.1%) than in 1986&#x2013;1999 (29.2%, 22.6%).</p>
<p>At the seasonal time scale, human activities had a relatively larger contribution to streamflow changes during dry seasons than in wet seasons, such as the value of Jiu-Yun area between 1986 and 2000, where the <inline-formula id="inf34">
<mml:math id="m45">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> accounted for 27.6% during the dry season but 13.1% in the wet season. Additionally, in the V-S area between 1986 and 1999, human activity accounted for 68.3% during the dry season but 62.0% during the wet season.</p>
</sec>
<sec id="s3-4">
<title>3.4 Impact of land use type change on streamflow in different areas</title>
<p>In terms of <xref ref-type="disp-formula" rid="e10">Formulas (10)</xref> and <xref ref-type="disp-formula" rid="e11">11</xref>and the control scenarios in <xref ref-type="table" rid="T3">Table 3</xref>, further quantitative assessment of the impact from land use type changes (<inline-formula id="inf35">
<mml:math id="m46">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>) in Jiuzhou, Jiu-Yun, Yun-V, and V-S areas on the annual and seasonal streamflow could be analyzed. The detailed analysis and contributions of land use type changes during 1990&#x2013;2015 are shown in <xref ref-type="fig" rid="F7">Figure 7</xref> and <xref ref-type="table" rid="T4">Table 4</xref>. Land use change is often caused by climate change and human activities; however, according to previous studies (<xref ref-type="bibr" rid="B32">Tang et al., 2021</xref>; <xref ref-type="bibr" rid="B6">Ep et al., 2021</xref>), human activities played a major role in the LMRB. Therefore, we did not take the impact of climate on land use types into consideration here.</p>
<fig id="F7" position="float">
<label>FIGURE 7</label>
<caption>
<p>The situation of Land use types change in Lancang-Mekong River Basin.</p>
</caption>
<graphic xlink:href="feart-10-1024037-g007.tif"/>
</fig>
<table-wrap id="T4" position="float">
<label>TABLE 4</label>
<caption>
<p>The contributions of land use types change to annual and seasonal streamflow changes in Lancang-Mekong River Basin.</p>
</caption>
<table>
<thead valign="top">
<tr>
<th align="center">Station</th>
<th align="center">Period</th>
<th align="center">
<inline-formula id="inf36">
<mml:math id="m47">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</th>
<th align="center">Station</th>
<th align="center">Period</th>
<th align="center">
<inline-formula id="inf37">
<mml:math id="m48">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</th>
<th align="center">Station</th>
<th align="center">Period</th>
<th align="center">
<inline-formula id="inf38">
<mml:math id="m49">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</th>
<th align="center">Station</th>
<th align="center">Period</th>
<th align="center">
<inline-formula id="inf39">
<mml:math id="m50">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>
</th>
</tr>
</thead>
<tbody valign="top">
<tr>
<td rowspan="6" align="center">Jiuzhou</td>
<td align="center">A<sub>1</sub>
</td>
<td align="char" char=".">5.88%</td>
<td rowspan="6" align="center">Jiu-Yun</td>
<td align="center">A<sub>2</sub>
</td>
<td align="char" char=".">9.68%</td>
<td rowspan="6" align="center">Yun-V</td>
<td align="center">A<sub>3</sub>
</td>
<td align="char" char=".">20.67%</td>
<td rowspan="6" align="center">V-S</td>
<td align="center">A<sub>4</sub>
</td>
<td align="char" char=".">14.99%</td>
</tr>
<tr>
<td align="center">B<sub>1</sub>
</td>
<td align="char" char=".">4.79%</td>
<td align="center">B<sub>2</sub>
</td>
<td align="char" char=".">7.48%</td>
<td align="center">B<sub>3</sub>
</td>
<td align="char" char=".">22.21%</td>
<td align="center">B<sub>4</sub>
</td>
<td align="char" char=".">30.12%</td>
</tr>
<tr>
<td align="center">A<sub>1</sub>-dry</td>
<td align="char" char=".">8.95%</td>
<td align="center">A<sub>2</sub>-dry</td>
<td align="char" char=".">17.67%</td>
<td align="center">A<sub>3</sub>-dry</td>
<td align="char" char=".">16.63%</td>
<td align="center">A<sub>4</sub>-dry</td>
<td align="char" char=".">30.67%</td>
</tr>
<tr>
<td align="center">B<sub>1</sub>-dry</td>
<td align="char" char=".">4.68%</td>
<td align="center">B<sub>2</sub>-dry</td>
<td align="char" char=".">10.29%</td>
<td align="center">B<sub>3</sub>-dry</td>
<td align="char" char=".">20.70%</td>
<td align="center">B<sub>4</sub>-dry</td>
<td align="char" char=".">36.66%</td>
</tr>
<tr>
<td align="center">A<sub>1</sub>-wet</td>
<td align="char" char=".">7.74%</td>
<td align="center">A<sub>2</sub>-wet</td>
<td align="char" char=".">2.75%</td>
<td align="center">A<sub>3</sub>-wet</td>
<td align="char" char=".">14.48%</td>
<td align="center">A<sub>4</sub>-wet</td>
<td align="char" char=".">11.57%</td>
</tr>
<tr>
<td align="center">B<sub>1</sub>-wet</td>
<td align="char" char=".">4.87%</td>
<td align="center">B<sub>2</sub>-wet</td>
<td align="char" char=".">6.87%</td>
<td align="center">B<sub>3</sub>-wet</td>
<td align="char" char=".">27.46%</td>
<td align="center">B<sub>4</sub>-wet</td>
<td align="char" char=".">22.68%</td>
</tr>
</tbody>
</table>
<table-wrap-foot>
<fn id="Tfn2">
<label>
<sup>a</sup>
</label>
<p>A-dry and A-wet are short for the period of dry and wet seasons, respectively.</p>
</fn>
</table-wrap-foot>
</table-wrap>
<p>In Jiuzhou, each area of land use types remained stable except from a small proportion reduce of the grassland from 1990 to 2000, so the <inline-formula id="inf40">
<mml:math id="m51">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
<mml:mi>c</mml:mi>
<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> on long-term streamflow is under 6%. In Jiu-Yun area, because of there were more farmland and urban area than in Jiuzhou, but small land use types change, the <inline-formula id="inf41">
<mml:math id="m52">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mrow>
<mml:mi>l</mml:mi>
<mml:mi>u</mml:mi>
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<mml:mi>c</mml:mi>
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</mml:msub>
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</inline-formula> accounts for 9.68% and 7.48% in A<sub>2</sub> and B<sub>2</sub>, respectively. In Yun-V area, the areas of forest land decreased by 6% and farmland increased by 4% from 1990 to 2010. Therefore, the contribution from land use type changes showed a relatively high proportion (20.67%) in A<sub>3</sub> and 22.21% in B<sub>3</sub> at the yearly time scale. The same situation occurred in V-S area, where the area of forest land decreased by 7% and farmland increased by 6% from 1990 to 2010. Correspondingly, the yearly contribution from land use type changes remained at a high level, at 14.99% and 30.12% in A<sub>4</sub> and B<sub>4</sub>, respectively. Furthermore, the dry seasons were easier to be affected in the most area of LMRB than in wet seasons.</p>
</sec>
<sec id="s3-5">
<title>3.5 Discussion</title>
<sec id="s3-5-1">
<title>3.5.1 Uncertainties in the simulation</title>
<p>Although this study provides a quantitative evaluation of the impacts of climatic variation and human activities on streamflow changes in the integrated form in LMRB and the SWAT model could well simulate the natural streamflow, there are still some uncertainties in the model simulations (<xref ref-type="bibr" rid="B27">Sangam et al., 2022</xref>). The uncertainty mainly comes from the reference and change periods judgement, the model structure setting, input data and parameter calibration (<xref ref-type="bibr" rid="B12">Han and Zheng, 2016</xref>; <xref ref-type="bibr" rid="B4">Chen et al., 2022</xref>). First, this study judged the mutation year by cumulative anomalies of streamflow series, but different assumptions and methods may lead to different changing points, further affecting the follow-up analysis. Second, SWAT model assumes that each HRU has the same slope, which may affect the flow production and confluence simulation of different land types. Third, multi-source meteorological data such as reanalysis data and interpolated data were used as the input data in the simulation process, which may cause bias to some extent (<xref ref-type="bibr" rid="B17">Li et al., 2022</xref>; <xref ref-type="bibr" rid="B28">Sudesan et al., 2022</xref>). Finally, our model was calibrated and validated based on only observed streamflow data, and thus, the simulation of evaporation and infiltration need to be further validated after the calculation of observed data (<xref ref-type="bibr" rid="B10">Gowda et al., 2022</xref>).</p>
</sec>
<sec id="s3-5-2">
<title>3.5.2 Limitations of this research</title>
<p>Due to the large span of time-space and great differences in geographical conditions in the LMRB, the observed data was difficult to be obtained. The limitation was related to three aspects. The first limitation is that the streamflow data in the most of the stations in LMRB were absent and inconsecutive, which lead to the streamflow simulation of estuary area was not been achieved. The second limitation is that the human activities such as the reservoir discharge and irrigation water were not considered in this study. Reservoirs, such as the Xiao wan and Nuozhadu hydropower stations in LRB may affect the simulation result in certain years (<xref ref-type="bibr" rid="B38">Wang et al., 2017</xref>). The third limitation is that this study used muti-source data in the format like site in LRB &#x2b; grid in MRB instead of all grid data (<xref ref-type="bibr" rid="B13">Han et al., 2012</xref>; <xref ref-type="bibr" rid="B33">Tang et al., 2019</xref>). Through comparing the correlation between these two data forms and the observed streamflow series, using the site data in LRB could ensure the accuracy of streamflow simulation and the process of calibration and validation to the maximum extent (<xref ref-type="bibr" rid="B29">Swain et al., 2018</xref>; <xref ref-type="bibr" rid="B34">Tang et al., 2021</xref>).</p>
</sec>
</sec>
</sec>
<sec sec-type="conclusion" id="s4">
<title>4 Conclusion</title>
<p>As the largest transboundary river in Asia, the Lancang-Mekong River (LMR) not only connects six countries with significant geographical, economic, and cultural differences, but also spans the alpine, temperate, and tropical climatic zones, making the Lancang-Mekong River Basin (LMRB) suffer significant influences from climate change and human activities. This study performed a detailed analysis of the impacts of climate and human activities across the entire LMRB on streamflow process over the recent decades in an integrated form using a large number of multi-source data, trend analysis and cumulative anomaly methods and SWAT model. The main results obtained in this study are:<list list-type="simple">
<list-item>
<p>1) At the yearly timescale, there is no significantly increasing or decreasing trend showing in the streamflow process at Jiuzhou station, but a significant decrease at Yunjinghong station, an insignificant decreasing trend at Vientiane station and an increasing trend at Stung Treng station. At the seasonal timescale, the varying trend of streamflow at each hydrological station was consistent essentially with that at the yearly timescale.</p>
</list-item>
<list-item>
<p>2) At the yearly timescale, the ratios of the contributions of human activities (&#x3b7;<sub>h</sub>) to the streamflow changes before 2000 to the counterparts after 2000 are 15.2% and 10.3%, 17.5% and 17.1%, 29.2% and 32.4%, 22.6% and 59.1% in Jiuzou, Jiu-Yun, Yun-V and V-S areas, respectively. At the seasonal timescale, &#x3b7;<sub>h</sub> in the dry season before to after 2000 is 17.8% and 10.5%, 27.6% and 35.7%, 28.5% and 29.3%, 56.1% and 68.3%, respectively. In the wet season, &#x3b7;<sub>h</sub> is 13.9% and 9.5%, 13.1% and 13.5%, 27.1% and 39.4%, 22.8% and 62%, respectively.</p>
</list-item>
<list-item>
<p>3) The impacts of climate change on streamflow in the LMRB gradually decreased from the upper to the lower reaches, while the impacts of human activities gradually increased. Furthermore, the impacts of climate change on streamflow in the dry season were more significant than in the wet season. The contributions of Land use types change (<inline-formula id="inf42">
<mml:math id="m53">
<mml:mrow>
<mml:msub>
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<mml:mrow>
<mml:mi>l</mml:mi>
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<mml:mi>c</mml:mi>
</mml:mrow>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula>) account for about 1/3 of <inline-formula id="inf43">
<mml:math id="m54">
<mml:mrow>
<mml:msub>
<mml:mi>&#x3b7;</mml:mi>
<mml:mi>h</mml:mi>
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</mml:mrow>
</mml:math>
</inline-formula> in LRB but more than 1/2 of <inline-formula id="inf44">
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<mml:mrow>
<mml:msub>
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<mml:mi>h</mml:mi>
</mml:msub>
</mml:mrow>
</mml:math>
</inline-formula> in MRB.</p>
</list-item>
</list>
</p>
<p>Although this study provides a quantitative evaluation of the impacts of climatic variation and human activities on streamflow changes in the integrated form in LMRB, some shortcomings remain. While this study took the effects of climate, topography, soil, vegetation, and other spatial-temporal variability on streamflow into consideration, the parameter values, sensitivity, and scale effects of those factors on streamflow process were not systematically investigated and further detailed studied are required (<xref ref-type="bibr" rid="B1">Ahn and Merwade, 2014</xref>; <xref ref-type="bibr" rid="B8">Gao et al., 2020</xref>; <xref ref-type="bibr" rid="B3">Andaryani et al., 2021</xref>; <xref ref-type="bibr" rid="B37">Wang et al., 2021</xref>).</p>
</sec>
</body>
<back>
<sec sec-type="data-availability" id="s5">
<title>Data availability statement</title>
<p>Publicly available datasets were analyzed in this study. This data can be found here: 1) Meteorological data. Daily precipitation and temperature data of the LMRB were collected from the Chinese meteorological data sharing service system (available from <ext-link ext-link-type="uri" xlink:href="http://data.cma.cn/data/detail/dataCode/A.0012.0001.html">http://data.cma.cn/data/detail/dataCode/A.0012.0001.html</ext-link>) and the Asian Precipitation-Highly-Resolved Observational Data Integration Towards Evaluation (APHRODITE, 0.25&#xb0; &#x2179; 0.25&#xb0;, available from <ext-link ext-link-type="uri" xlink:href="http://aphrodite.st.hirosaki-u.ac.jp/download/">http://aphrodite.st.hirosaki-u.ac.jp/download/</ext-link>), respectively. To compensate for the lack of APHRODITE data at the maximum and minimum temperature, this study used the Global Surface Summary of the Day (GSOD, available from <ext-link ext-link-type="uri" xlink:href="https://data.noaa.gov/dataset/dataset/global-surface-summary-of-the-133 day-gsod">https://data.noaa.gov/dataset/dataset/global-surface-summary-of-the-133 day-gsod</ext-link>) data from NOAA and Global Historical Climatology Network (GHCND, available from <ext-link ext-link-type="uri" xlink:href="https://www.ncei.noaa.gov/products/land-based-station/global-historical-climatology-network-daily">https://www.ncei.noaa.gov/products/land-based-station/global-historical-climatology-network-daily</ext-link>) data. Evapotranspiration was calculated using the Penman-Monteith equation. 2) Hydrological and topographic data. The streamflow data were provided by Yunnan University and the Mekong River Commission (MRC). The geographical data, such as the 1&#xa0;km &#xd7; 1&#xa0;km raster data, was extracted and resampled from 90&#xa0;m resolution digital elevation model (DEM) data provided by the USGS (available from <ext-link ext-link-type="uri" xlink:href="http://www.usgs.gov">http://www.usgs.gov</ext-link>). 3) Land cover and soil data. The land use and land cover data of the LRB (1980&#x2013;2015, per 5&#xa0;years) were produced by the Institute of Geographical Sciences and Resources, Chinese Academy of Sciences, and the similar data of the MRB were downloaded and resampled from the Servir-Mekong dataset from 1987 to 2015 (available from <ext-link ext-link-type="uri" xlink:href="https://rlcms-servir.adpc.net/en/landcover">https://rlcms-servir.adpc.net/en/landcover</ext-link>). Soil data in LMRB was obtained from the Harmonized World Soil Database (HWSD).</p>
</sec>
<sec id="s6">
<title>Author contributions</title>
<p>Conceptualization, RL and HH; methodology, RL and RZ; software, RL and ZW; validation, RL and HH; formal analysis, RL and HH; investigation, RL, HH, and ZW; resources, HH and RZ; data curation, RL; writing&#x2014;original draft preparation, RL; writing&#x2014;review and editing, RL and HH; visualization, RL and HH; supervision, HH and ZW; project administration, HH and ZW; funding acquisition, ZW and HH.</p>
</sec>
<sec id="s7">
<title>Funding</title>
<p>This research was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA19030204) and National Natural Science Foundation of China (Grant No. 41,561,144,012).</p>
</sec>
<sec sec-type="COI-statement" id="s8">
<title>Conflict of interest</title>
<p>The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.</p>
</sec>
<sec sec-type="disclaimer" id="s9">
<title>Publisher&#x2019;s note</title>
<p>All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.</p>
</sec>
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