Abstract
The activation of plant defense systems in response to herbivory or experimentally applied methyl jasmonate (MeJA) involves the production of chemical defense substances functioning as warning signals to repel herbivores and protect against pathogens. They also serve as signals detectable by undamaged neighboring plants, a phenomenon called plant–plant communication. We studied how altitudinal variation in temperature and timing of snowmelt affected herbivore resistance, growth and reproduction of untreated bilberry (Vaccinium myrtillus L.) 20–500 cm from MeJA-treated ramets. Across 2 years, responses of MeJA-treated and untreated bilberry ramets were recorded twice per season along an elevational gradient in a boreal system. At low and medium altitudes, untreated bilberry showed increased herbivore resistance and reduced growth and reproduction up to 500 cm from MeJA-treated ramets. In the warmer sites at these altitudes, the effects persisted for 2 years for ramets up to 100 cm from the treated ramets. At high altitudes, however, only untreated ramets growing 20–100 cm from the treated ramets showed increased resistance to insect herbivores and reduced reproduction, but these effects did not persist into the second year. Altitudinal variation in climate affected trade-offs between plant defense, growth, and reproduction. Our findings indicate that plant–plant communication is also influenced by the combination of changes in climate and time after induction. Adaptations of plants growing under increasing temperature in high-latitude environments can profoundly impact ecosystem functioning, especially where bilberry, the key plant species in the boreal system, interacts with its herbivores.
Introduction
Global warming is currently occurring at an unprecedented rate (). Mean global temperature is projected to increase by 1.5–2.0°C in coming decades, causing longer drought periods in some areas and more precipitation in others (). Climate change is likely to be a major threat to biodiversity in many regions worldwide (; ). High latitude tundra and boreal areas are particularly vulnerable to climate change-induced degradation and loss, with woody shrubs encroaching onto the tundra (). At northern latitudes, milder winters and warmer summers will prolong the growing season, alter plant growth patterns, and eventually cause a mismatch between, for example, herbivore larvae emergence and bud burst, and leafing in host plants (; ; ). Climate change is also expected to influence plant phenology (differences in growth and maturation), physiology (e.g., stomatal functioning in plants and the physicochemical properties of volatiles compounds), and resource allocation from growth or reproduction, or both, to defense when under herbivore attack. These can have direct and indirect implications for natural ecosystem functioning (; ).
Plant coexistence can influence interactions with organisms such as herbivores, predators, pollinators, and microorganisms. Indeed, a plant’s association with its neighbors can take several forms, with competitive and facilitative interactions playing fundamental roles in shaping natural communities. Plant–plant competition has long been a key component of many classic ecological theories (). In contrast, the importance of positive plant–plant interactions, such as facilitation, has only more recently been recognized as being equally important in regulating community composition and ecosystem functioning (; ; ). Neighboring plants can facilitate each other by promoting their growth (), for example, through fixing nitrogen (), or helping each other in defense against herbivores either directly, through reducing damage by diverting herbivores from their host plant (), or indirectly, by attracting the herbivores’ natural enemies (). These negative and positive plant–plant interactions can be mediated by biotic (i.e., herbivore attack) and abiotic factors (i.e., environmental drivers) (; ). However, little is known about the effects of climate in modulating these interactions.
Plants under attack by herbivores and pathogens activate inducible defense mechanisms such as the emission of volatile organic compounds (VOCs) from aboveground and belowground tissues (; ; ). Once VOCs are emitted, however, they convey ‘public’ information that can be perceived by surrounding plants and organisms. For instance, herbivore-induced VOCs can attract natural enemies of herbivores (), provide protection against pathogens (), and repel herbivores (), potentially defending plants against their enemies (). Moreover, undamaged neighboring plants can recognize VOCs emitted from attacked plants and use them as a cue to mobilize their own defense systems (; ; ). Plant communication can be influenced by climate because VOC production, volatility and, consequently, VOC movement at the leaf surface, are controlled by temperature (). Recent studies highlight possible effects of climate change on the biosynthesis and abundance of VOCs (; ), which could mitigate their ecological functions (). Higher VOC production and volatility under elevated temperatures () are expected to change plant–herbivore interactions by altering the ability of specialist herbivores to locate their hosts, thereby increasing antiherbivore defense responses and modifying plant–plant interactions (Yuan et al., 2009). However, we still do not fully understand how inter-plant communication mechanisms vary under different climate regimes, how these could be affected by changing environmental conditions, and what the consequences could be for ecological interactions and ecosystem functioning.
Plants incur costs if limiting resources, such as nitrogen and carbon, are invested in defense rather than biomass and fruit production (). Studies have shown that the activation of plant defense systems are energetically costly and reduce plant growth and reproduction (; ; ; ). As plant defense responses induced by the phytohormone methyl jasmonate (MeJA) are generally similar to those induced by insect herbivory (; ), the application of exogenous MeJA is a useful tool to stimulate plant resistance in studies of plant–herbivore interactions. MeJA can trigger the re-allocation of energy from plant primary metabolism to the activation of plant defensive genes (). Plant defense responses to MeJA include the production of various secondary metabolites and anti-digestive proteins such as proteinase inhibitors, which can harm both specialist and generalist herbivores (; ; ; ). MeJA has been also shown to induce the emission of plant volatiles that affect the plants’ interactions with individuals of the same trophic level (i.e., neighboring plants) as well as those from higher trophic levels such as the attraction of natural enemies of herbivores (; ; ). For example, in bilberry (Vaccinium myrtillus L.), a key food source for many herbivore species in boreal forests, treatment with MeJA increases resistance to herbivores and reduces plant growth and reproduction for several years (i.e., prolonged multiannual defense) (). These trade-offs between herbivore resistance and both growth and reproduction have previously been observed on MeJA-treated bilberry plants as well as on untreated neighbors (). Given that ecosystem composition and functioning can affect the ecosystem services on which human society depends (; ), there is a strong practical need to understand those processes that regulate biodiversity, particularly in the light of potential impacts of climate change. It is therefore timely to consider how variations in climate might modulate plant–plant interactions and their effects, especially in high-latitude ecosystems.
In this study, we investigate how altitudinal variation in temperature and the time of snowmelt affect growth, reproduction and the induction of defenses in MeJA-treated and untreated neighboring bilberry ramets. Because VOCs can induce defenses in neighboring plants some distance away (; ; ), we predict similar patterns of defense in MeJA-induced and untreated neighboring bilberry ramets at different distances from the induced plant, for example, reduced growth, reproduction, and herbivory. Such a distance effect would indicate plant–plant communication. Furthermore, because plant-induced defenses appear to decline with increasing elevation () and elevated temperature is known to increase biogenic VOC emissions (; ), we expect to find stronger plant–plant communication effects in the warmer, lower altitude environments.
Materials and Methods
Study System
We conducted this study along an elevational gradient in the boreal pine-bilberry forest ecosystems of Kaupanger, the inner Sognefjord, western Norway (61.2°N, 007.2°E) in 2016 and 2017. Annual precipitation there is 700–900 mm with a mean summer temperature range of 12–16°C (). The most abundant tree is pine (Pinus sylvestris L.), with dwarf shrubs, such as bilberry, lingonberry (Vaccinium vitis-idaea L.), and crowberry (Empetrum nigrum L.) dominating the field layer. Bilberry is a long-lived deciduous clonal dwarf shrub with evergreen stems usually 10–60 cm high (; ). Although we do not have specific information on clone size and distribution in the study area, we have based our study on the assumptions that rhizomes can reach approximately 200 cm in length, depending on age, and that there is considerable genetic variation within the population (; , ). Bilberry is considered a key plant in boreal forests because it is a food source for many vertebrate and invertebrate herbivores (). The main mammalian herbivores feeding on bilberry in this area are red deer (Cervus elaphus L.) and various rodent species, whereas the commonest insect herbivores are Geometridae larvae (S. J. Hegland, personal observation), and the main visitors to bilberry flowers are bumblebees, solitary bees, as well as ants (S. J. Hegland, unpublished data).
Gradient Design
Inner Sognefjord is topographically diverse with marked altitudinal gradients occurring within a relatively small area. These are ideal for climate research, making it practical and convenient to carrying out ecological experiments over short geographical distances. We used a natural altitudinal gradient ranging from just above sea level to the subalpine zone to investigate the effects of variation in climate, mainly temperature and the timing of snowmelt, on plant–herbivore interactions and plant–plant communication. Anorthosite bedrock and moraine material dominate the whole gradient () and there are no latitudinal or longitudinal influences. As such this gradient design reduces the possibility of confounding effects that can often be a challenge in climate-gradient studies.
We conducted an experiment at three different elevations representing an altitudinal gradient along which temperature, timing of snowmelt and length of the growth season varied (Figure 1): ‘Low’ elevation, ca. 100 m a.s.l. (submontane zone); ‘Medium’ elevation, ca. 500 m a.s.l. (mid-montane zone); and ‘High’ elevation, ca. 900 m a.s.l. (subalpine zone). We placed four data loggers (TRIX 8 LogTag, Auckland, New Zealand) at each site to record data on average temperature and relative humidity throughout the study season in both years. We recorded the timing of snowmelt as the Julian day on which each site became largely snow free. All experimental sites faced south west, thereby reducing incidental environmental variation, such as light conditions. Low and Medium sites consisted of more than 10-year-old clear-cuts with small pines, birches and alders that produced negligible shadow. The High site was a naturally open subalpine area just below the tree line. Unpublished data from 2016 showed that there was relatively little variation in pH (mean, 4.19) and slope (16.4 degrees) among sites, although soil organic matter (mean, 60.4%) was slightly lower at the low altitude (Knut Rydgren, personal communication 2016). Vegetation structure was similar among the sites, with a field layer and scattered tree layer up to ca. 5 m. In total, the sites represent similar habitat characteristics in regard to factors that are not under direct control of climate – such as shading, slope, and soil parameters.
FIGURE 1
Experimental Design and Data Collection
In May and June 2016, at each site (Low, Medium, and High), we established 20 150 m2 (10 m × 15 m) blocks, leaving a minimum of 10 m between each to avoid interference from neighboring blocks. Within each block, two transects were established at least 10 m apart with five individually marked bilberry ramets in each, ranging in height from 10 to 25 cm. We located the five ramets at 10–30 cm (called dist. 20 hereafter), 40–60 cm (dist. 50), 80–120 cm (dist. 100), and 400–530 cm (dist. 500) from the first ramet (dist. 0) in each transect (Figure 2). Treated and control transects were randomly assigned within each block. The first ramets (dist. 0) were subjected to one of two treatments: 10 mM MeJA application, termed the MeJA-treated or focal ramet (treated transect), or a water: ethanol application (control transect). To achieve the desired concentration of MeJA, 4.1M MeJA stock solution (Bedoukian Research, Danbury, CT, United States) was diluted 1:10 with 95% (v/v) ethanol and diluted further with water to get a final concentration of 10 mM MeJA (). Ethanol was added to water at the same final concentration as that in the 10 mM MeJA solution (41:1) for the control. To avoid rapid evaporation of MeJA, a cotton wad was attached to the stem at the ground and saturated with 10 mM MeJA (treatment) or water-ethanol (control) to the point of run-off. This was repeated three times at 1-week intervals after the initial application to simulate attack by herbivores. This MeJA concentration (10 mM) and application method and timing were shown in our earlier studies to activate defenses in the treated bilberry plants as well as in conspecific neighboring plants under similar natural settings (; , , ). The ramets were not exposed to further treatments in 2017, to reveal any possible multiannual effects. To evaluate possible plant–plant communication between MeJA-treated and untreated neighboring ramets, only the first ramet (dist. 0) in the transect was treated (). The other ramets along the transects served as test subjects.
FIGURE 2
We used a similar sampling procedure to that adopted in a previous study (). In May/June 2016 (sampling time 1 [ST1]), before the start of treatment at all sites, we measured ramet height from the ground to crown with a ruler, and stem diameter at ground level with a digital caliper, for all the marked plants on the transects. We also counted the number of annual shoots, flowers, leaves, browsed shoots and insect-chewed leaves. We measured the same variables again at the end of the season (sampling time 2 [ST2]), 6 weeks (42 days) after the start of treatment. On this latter occasion, we also counted the number of berries. The same procedure was repeated in 2017 except that the focal plants and controls were not treated then. Plant height (H), stem diameter (DS), and the number of shoots (AS) were used to calculate the dry mass (DM) of each ramet, applying the formula proposed by : log2(DM) = 1.41700 × log2(DS) + 0.97104 × log2(H) + 0.44153 × log2(AS + 1) - 7.52070. This provided a non-destructive estimate of plant size.
Data Analyses
We assessed if MeJA induced anti-herbivore defenses in wild bilberry ramets by analyzing the patterns of herbivory (proportion of chewed leaves by insect herbivores and proportion of mammalian-browsed shoots), growth (changes in biomass, through dry mass calculation), and fruit set (proportion of berries to flowers) of MeJA-treated and untreated neighboring ramets at different distances, across an elevational gradient in two consecutive seasons. The analysis involved comparing control ramets with the corresponding ramets in the experimental MeJA-treated transects (dist. 0), and for untreated neighboring ramets at increasing distance from the control and experimental ramets (dist. 20–500), at each site along the elevational gradient in 2016 and 2017. The response variables were the changes in the proportion of chewed leaves, proportion of browsed shoots, and biomass, calculated by subtracting the values at ST1 (beginning of the season) from those at ST2 (end of the season). These provided measures of seasonal change for each year. Effects on reproduction were analyzed yearly, based on measurements of the number of flowers present in May–June and the number of berries in August–September in each year, from which fruit set was calculated as the proportion of berries to flowers.
For each response variable, we used the lme4 () and mixlm () libraries in to fit generalized linear mixed effect models (GLMM) with Gaussian error distribution with identity link and performed posterior analysis of variance (ANOVA). For all models, in each year (2016 and 2017), we entered treatment (control and MeJA dist. 0–500) and site (Low, Medium, and High), with interaction terms as fixed effects. Blocks within each elevational site were fitted as random effects. To account for variation in plant size, the following covariates were included: total number of leaves at ST1 (for insect herbivory model), total number of shoots at ST1 (for mammalian herbivory model), total biomass at ST1 (for growth model), and total number of flowers at ST2 (for the fruit-set model). The treatment effects on reproduction were analyzed yearly, based on estimates of fruit set as defined above. Visual inspection of residual plots did not reveal any obvious deviation from homoscedasticity or normality for any model. To determine the presence of an interaction effect, we performed likelihood ratio tests of the full model against the model without the effect or interaction in question. We assessed the statistical significance (P-values) of single factors and their respective interactions by performing ANOVA for each model. Differences and mean separations between control and MeJA (dist. 0–500) ramets, within each elevational site, were analyzed by Tukey post hoc comparisons (α = 0.05) (Supplementary Tables S1, S2).
Results
Insect herbivory, measured as the change in the proportion of chewed leaves, was significantly reduced in MeJA-treated and untreated neighboring bilberry ramets at all distances (dist. 20–500) at the Low and Medium sites, and up to dist. 100 at the High site in 2016, compared with that of control ramets. This reduction in insect herbivory was maintained in 2017, but only in MeJA-treated ramets and in untreated neighbors up to dist. 50 at the Low and Medium sites, and not at all at the High site (Figure 3 and Supplementary Table S1).
FIGURE 3
Mammalian herbivory, measured as the change in the proportion of browsed shoots, was similarly significantly reduced in both years in MeJA-treated and neighboring ramets at all distances at both Low and Medium sites, compared with that in control plants. At the High site, however, there was no statistically significant reduction in browsing pressure other than in MeJA-treated ramets (dist. 0), and then only in 2016 (Figure 4 and Supplementary Table S1).
FIGURE 4
In 2016, seasonal growth (change in biomass) was reduced in MeJA-treated and nearby neighboring ramets (dist. 20–50) at both Low and Medium sites compared with the control plants, but no significant difference was observed at the High site. A year after treatment (2017), biomass production continued to be significantly depressed relative to control plants at the Low site at distances up to ca. 50 cm, and at all distances at the Medium site. At the High site, however, which had displayed no significant response in 2016, significant reductions in growth were recorded in 2017 in the 2016 MeJA-treated experimental ramets and their closest neighbors (dist. 20), relative to the control (Figure 5 and Supplementary Table S2).
FIGURE 5
Fruit set, measured as the proportion of berries to flowers, was significantly reduced in MeJA-treated and untreated neighboring ramets at distances from ca. 20 to 100 cm at both Low and High sites in 2016. The effect of MeJA-application was more marked at the Medium site where reduced fruit set was recorded in untreated neighbors up to ca. 500 cm away (Figure 6 and Supplementary Table S2). Reduced fruit set was recorded for MeJA-treated and untreated neighboring ramets in 2017 at the Low and Medium sites at distances up to ca. 100 cm, but not in any of the plants at the High site at any distance (Figure 6 and Supplementary Table S2).
FIGURE 6
Discussion
Our results show that treated plants and untreated neighbors up to 500 cm away appear to have activated their defense systems in response to treatment with MeJA, as shown by reductions in both insect and mammalian herbivory, relative to control plants in the same area. This in turn resulted in reduced growth and reproduction in these MeJA-treated plants and their untreated neighbors, presumably due to the reallocation of resources from growth and reproduction to defense. This effect was observed at all elevations, but was strongest at Low and Medium altitude sites, where the effect persisted into a second year, in line with our predictions. At these sites the effects were more strongly induced and persistent also among untreated conspecific ramets at intermediate to long distances (ca. 100–500 cm). Conversely, at the High site, where temperatures are lower and the growing season is shorter, only MeJA-treated and untreated neighboring ramets at short to intermediate distances (ca. 20–100 cm) showed reduced herbivory and fruit set, and this effect did not persist into the next year. The single instance of reduced growth recorded in 2017 at the High site in the 2016 MeJA-treated experimental plants and close neighbors (dist. 20) may have been coincidental and could have arisen if the plants were responding to an instance of natural herbivory and the release of volatile defense compounds.
We found clear neighborhood/distance effects of MeJA-treatment on bilberry growth, reproduction, and herbivory resistance across all elevations in both MeJA-treated and untreated neighboring ramets. These effects were largely as predicted across elevation and time, and support the idea of plant–plant communication, with both MeJA-treated and untreated neighboring ramets up to 5 m away evidently activating their defense systems, at least in 2016 when the plants were treated. Our results are consistent with other studies showing that above- (; ; ; ) and belowground (; ; ; ) signals emitted by induced plants can activate the defense system of uninduced neighbors (; ; ; ; ). Moreover, these effects were more pronounced and consistent in the sub-montane and mid-montane climatic zones (Low and Medium sites) than in the subalpine zone (High sites), suggesting that plant–plant communication in bilberry, either through airborne or belowground signaling from MeJA-treated ramets, is apparently modulated by climatic conditions.
The reduction in growth, fruit set, and levels of insect and mammalian herbivory, in untreated bilberry ramets growing within about 5 m of MeJA-treated ramets suggests that there is a trade-off between growth and defense. This trade-off apparently persisted, albeit less markedly, for at least the next year in untreated neighboring plants up to ca. 100 cm from the treated ramets at the Low and Medium sites, suggesting that defense activation of induced plants and their neighbors growing in sub-montane to mid-montane zones in the boreal forest is multiannual. In a previous study, in the same ecosystem and area, at ca. 350 m a.s.l., we also found evidence of multiannual plant–plant communication effects between MeJA-treated and untreated neighboring ramets ().
Although MeJA-treated and untreated bilberry ramets up to ca. 100 cm away showed reduced insect herbivory and fruit set initially at the High site, neither growth nor mammalian herbivory, other than on the treated ramets, were significantly reduced. Furthermore, in contrast to plants growing at lower elevations, these effects did not persist through to the next growing season, further evidence that climate impinges the effectiveness of plant–plant communication in an induced defense system. The elevational gradient used in our study parallels a natural climatic gradient and, as such, average temperatures together with relative humidity and consequent timing of snow melt at the Low and Medium sites are higher than at the High site, in what is effectively the subalpine zone. Plants growing at this latter zone experience a more stressful environment with lower average temperatures, later snowmelt, and, consequently, a shorter growing season than plants at lower altitudes in this boreal ecosystem. Overall, climate can be an important abiotic factor in determining the intensity and efficacy of induced plant defenses, for example, by affecting the release of plant volatiles (; ). Such effects can alter plant–herbivore and plant–plant interactions and can result in important changes in natural ecosystem functioning, such as cyclic fluctuations in herbivore populations ().
The relatively warmer environments at the Low and Medium elevation sites, together with their longer growth seasons, boosted the performance of the induced defense system in bilberry and produced more effective plant–plant communication. Although we have not determined the type of signaling, it is probable that both aboveground (airborne VOCs) and belowground (VOCs or ramet contact) processes were involved in activating the defense systems of untreated neighboring ramets. Internal and external factors can effect VOC emissions qualitatively and quantitatively (; , ; ; ; ; ; ; ). For instance, elevated temperature increases biogenic VOC emission by enhancing enzyme activity during synthesis, raising vapor pressure, and decreasing the resistance to diffusion (; ; ). Altered VOC emissions in a warmer environment can affect signaling efficacy directly in various ecological interactions in natural ecosystems such as plant–herbivore, plant–pollinator, and multi-trophic interactions (; ; Yuan et al., 2009), which can enhance the defense role of induced VOCs in warmer climates. Conversely, moderately elevated temperatures enhance feeding by certain herbivores (; ), thereby increasing inducible VOC emissions (; ). Besides temperature and other main environmental factors of climate change, VOC emissions from plants could also be altered by other abiotic factors, such as water, nutrient and resource availability, interacting with these main ones (; ; ), as well as altering phenology such as plant seasonality and leaf duration (). Our high-altitude site, located in the subalpine zone of the boreal forest biome, experienced cooler average temperatures, limited resource availability, and had a shorter growing season. This could result in lower VOCs emissions from MeJA-treated plants or lower response of non-induced plants to these VOCs in plants at higher altitudes, perhaps explaining their reduced plant–plant communication capacity compared with that of bilberry ramets growing at lower altitudes. Future studies are needed to confirm the involvement of VOCs (above and belowground) in plant–plant communication in bilberry and the changes in emissions with increasing elevation.
The more pronounced plant–plant communication found in bilberry ramets growing in the submontane (Low site) and mid-montane (Medium site) zones reflects the antiherbivore defense strategy of plants growing under milder environmental conditions. In a previous study using the same elevational gradient, our molecular and ecological data showed that high-altitude bilberry in the boreal forest invest more in constitutive defenses, whereas low-altitude plants rely more on induced defenses (). In that study by we also showed higher herbivory at lower altitudes compared to the subalpine site. Bilberry plants growing at lower altitudes, where herbivore pressure and seasonal temperatures are higher, appeared more responsive to simulated herbivore attack by diverting resources from growth and reproduction to induced antiherbivore defenses in both treated and untreated neighboring ramets. This suggests that bilberry plants, growing under warmer conditions and higher levels of herbivory, are adapted to be more flexible in their responses compared with conspecifics growing in subalpine zones. This flexibility has consequences both for natural ecosystem functioning, by modulating plant–herbivore interactions (e.g., outbreaks of herbivore populations), and for enhanced plant–plant interactions (e.g., detection and response to VOC emissions by adjacent plants).
In summary, our findings provide evidence for climate modulation of plant–plant communication as part of the induced plant defense system in bilberry. Low (submontane zone) to Medium altitude (mid-montane zone) environments in the boreal system, with higher average temperatures and longer growth seasons, favored induced defenses in the ramets of neighboring conspecifics, at least within ca. 5 m of a source plant. The greater prominence of this inducible plant defense system at these lower altitudes may presage the kinds of changes expected in plant–plant communication systems in a warmer world, which will likely feedback to further influence ecological functioning. Although challenging to perform, simulations of climate-change effects on plant–herbivore and plant–plant interactions in natural systems could help us predict the possible ecological consequences of a changing climate on vulnerable ecosystems, especially high-latitude systems in which there are marked dependences among species, such as bilberry and most insect and mammalian herbivores in the boreal forest.
Statements
Data availability statement
All datasets generated for this study are included in the article/Supplementary Material.
Author contributions
TS, SH, SM, and CR-S conceived the initial idea and designed field work. RB carried out the field experiments, collected, analyzed and interpreted the data, and wrote the manuscript. All authors contributed to critically reading and editing the manuscript, and they approved its final form.
Funding
This research was financially supported by the Norwegian Research Council (project no. 204403/E40), the Norwegian Environment Agency.
Acknowledgments
We thank Torbjørn Stokke, Sigurd Daniel Nerhus, and Mark Andrew Gillespie for their help with establishment and execution of the field experiment.
Conflict of interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fevo.2020.00117/full#supplementary-material
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Summary
Keywords
induced defenses, methyl jasmonate, neighborhood effects, plant–plant communication, Vaccinium myrtillus
Citation
Benevenuto RF, Seldal T, Moe SR, Rodriguez-Saona C and Hegland SJ (2020) Neighborhood Effects of Herbivore-Induced Plant Resistance Vary Along an Elevational Gradient. Front. Ecol. Evol. 8:117. doi: 10.3389/fevo.2020.00117
Received
27 January 2020
Accepted
14 April 2020
Published
08 May 2020
Volume
8 - 2020
Edited by
Maria L. Pappas, Democritus University of Thrace, Greece
Reviewed by
Paula Baptista, Polytechnic Institute of Bragança, Portugal; Rocio Escobar Bravo, University of Bern, Switzerland
Updates
Copyright
© 2020 Benevenuto, Seldal, Moe, Rodriguez-Saona and Hegland.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Rafael Fonseca Benevenuto, rfbenevenuto@gmail.com
This article was submitted to Chemical Ecology, a section of the journal Frontiers in Ecology and Evolution
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